Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"CRISPR-Cas9 editing"”.
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DESIGN AND INVESTIGATION OF DUMBBELL-SHAPED DNA MINIMAL VECTORS FOR CRISPR/CAS9 EDITING
… DNA vectors for the delivery of the CRISPR/Cas9 system. Using three targets, i.e. glucose-6-phosphate dehydrogenase (G6PD) Mahidol, G6PD Mediterranean and Southeast Asian Ovalocytosis, and a dual reporter assay, we demonstrated that dumbbell vectors offer a viable alternative to …
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Cas9-induced on-target genomic damage
CRISPR/Cas9 is the gene editing tool of choice in basic research and poised to become one in clinical context. However, current studies on the topic suffer from a number of shortcomings. Mutagenesis is often assessed using bulk methods, which means rare events go undetected, unresolved or are …
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Development of high-throughput nanoparticle screening technologies to facilitate the delivery of genetic therapies to non-liver cell types
Genetic drugs (such as siRNAs, mRNAs, and CRISPR/Cas9) have the potential to be curative therapies for countless diseases. However, gene therapies will only work if the genetic drug is delivered to the diseased cell type. One promising delivery method is through the use of Lipid Nanoparticles …
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Investigating the role of chromatin modifications in CRISPR/Cas9 gene editing
… and heterochromatin have been shown to impede CRISPR/Cas9 editing efficiency. Conversely, Cas9 can open previously inaccessible regions of DNA, and transcriptionally silent targets can usually be edited without difficulty. In order to address this paradox, we have developed a method that …
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The Interrogation of Cas9 Aptamers and sgRNA Structures Through SELEX
<p>While much of the current focus on advancing CRISPR-Cas9 editing revolves around the engineering of Cas9, the interrogation and evolution of sgRNA scaffold, in addition to novel Cas9 binding RNAs, represent another echelon of development and therapeutic potential. Currently, the majority of …
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Investigating the Molecular Mechanisms Underlying Ku Essentiality in Human Cells
… knockout TREx-293 cell line was created using CRISPR/Cas9 editing. I observed a loss of cell viability during Ku depletion but did not detect a decrease in telomere length using telomere restriction fragment assays. I used mass spectrometry to investigate proteomic changes caused by a Ku70 …
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Characterising the Role of the Calcium-dependent Citrullinating Enzyme Peptidyl Arginine Deiminase 2 in Ovarian Cancer
… used to examine the overexpression and knockout (CRISPR/Cas9 editing) of PADI2 expression, respectively, in the human-derived high-grade serous OVCAR-4 and mouse-derived EOC ID8-Luc2 cell lines. In OVCAR-4 cells, PADI2 overexpression reduced proliferation (22%) and cellular aggregation (94%), …
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Developing a new efficient CRISPR-Cas9 base Homology Directed Repair oriented gene editing plasmid for Streptomyces
The CRISPR-Cas9 editing mechanism revolutionized genetic engineering, providing a precise and versatile tool for editing the genomes of virtually any organism. Streptomyces are soil dwelling bacteria with a complex life cycle and a reputation for producing a wide array of bioactive compounds, …
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Developing molecular genetic tools for gene discovery in soybean (Glycine max)
… along with fast neutron mutagenesis, a dual gRNA CRISPR/Cas9 genome editing system was developed as a molecular genetic tool for gene discovery and crop improvement in soybean. In order to demonstrate the efficiency and ability to simultaneous by edit homeologous genes using a CRISPR/Cas9 system …
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Colouration evo-devo: the developmental basis of intra-specific variation in cichlid pigmentation
… egg-spot variation, I review the application of CRISPR/Cas9 technology to cichlid fish, defining the state-of-the-art of cichlid genome editing and synthesising resources. Next I establish CRISPR/Cas9 editing in A. calliptera: I report efficient editing and germline transmission of mutations, and …
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New approaches to antimalarial target deconvolution and measuring fitness effects of Plasmodium falciparum mutations implicated in drug resistance.
… problem, I set out to use BarSeq technology and CRISPR-Cas9 editing to create a pool of barcoded drug resistant mutants that covered a diverse representation of the currently known malaria resistome. These mutants would include three main groups: 1. Lines with clinical significance: mutations …
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A novel route to oncogenic activation of cell cycle kinase Aurora A
… AURKA expression levels. Accordingly, I used CRISPR/Cas9 editing to manipulate expression of endogenous AURKA mRNA for production of the short isoform only. This experiment revealed that impaired APA of AURKA mRNA is sufficient to cause AURKA overexpression and promote cancer-like cellular …