Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"CRISPR-Cas system"”.
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Uncovering New Mechanisms of Type III CRISPR-Cas System Inhibition and Effector Function
… numerous defense mechanisms. One such defense system is the bacterial adaptive immune system called CRISPR-Cas, which consists of clustered, regularly interspaced short palindromic repeats (CRISPRs) and a set of genes that encode CRISPR-associated (Cas) proteins. While there are seven distinct …
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Anti-CRISPRs and CRISPR-Cas: Characterization and Biotechnology
… phage adsorption, restriction modification systems, and the more recently discovered CRISPR-Cas immune systems. Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) loci together with their accompanying CRISPR-associated (Cas) genes form the only known bacterial adaptive defense …
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Two strategies to deepen knowledge the antibiotic resistance related traits in Italian MDR Staphylococci: comparative transcriptomic of two DAP-R/DAP-S MRSA isogenic couples and CRISPR system characterization of pathogenic S. epidermidis
… of diseases, ranging from mild skin forms to systemic forms, whilst S. epidermidis has been frequently implicated in endocarditis and infections of surgical implants and it seems to be the source for antibiotic resistance related gene acquisition by S. aureus. A comparative transcriptomic …
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Investigating Genetic (IN)Compatibility Between Temperate Phages and CRISPR-CAS Systems in Staphylococcus Aureus
… form of defense comprises the adaptive immune systems derived from clustered, regularly interspaced, short palindromic repeat (CRISPR) loci and CRISPR-associated (cas) genes. CRISPR-Cas immune systems enable the acquisition of heritable resistance to specific mobile genetic elements on the …
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Decipher in situ signaling and complex genetics with cellular recording and combinatorial perturbations
… Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated (Cas) system from prokaryotic adaptive immune system demonstrated unprecedented genome editing efficiency and programmability in sequence specific genome editing of mammalian cells. In this thesis, I utilized the CRISPR-Cas system …
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A novel CRISPR-Cas9 platform with divergent targeting capabilities
RNA-guided DNA endonucleases of the CRISPR-Cas system are widely used for genome engineering and thus have numerous applications in a wide variety of fields. The range of sequences that CRISPR endonucleases can recognize, however, is constrained by the need for a specific protospacer adjacent motif …
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A tripartite survey of CRISPR-Cas diversity: Assessing the contours and limits of natural variation in CRISPR-Cas systems by associated genes and spacer acquisition biases
Explorations of anti-phage defence systems, with a focus on CRISPR-Cas system diversity, in the past decade has enabled many significant advances in biotechnology especially in programmable gene editing platforms. Many previous investigations have employed guilt by association based data mining …
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A tripartite survey of CRISPR-Cas diversity: Assessing the contours and limits of natural variation in CRISPR-Cas systems by associated genes and spacer acquisition biases
Explorations of anti-phage defence systems, with a focus on CRISPR-Cas system diversity, in the past decade has enabled many significant advances in biotechnology especially in programmable gene editing platforms. Many previous investigations have employed guilt by association based data mining …
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CPISPR-CAS: From a Prokaryotic Immune System to a Gene Editing Tool
… Regularly Interspaced Short Palindromic Repeats (CRISPR) and their associated genes (cas) encode an adaptive, small-RNA-based immune system that protects prokaryotes from infectious phages and plasmids. CRISPR-Cas systems can be classified into three types based on their cas gene content. My …
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Recovering from Dormancy: Fixation of CSM6-Inducing Spacers in the Bacterial Population
… prokaryotes have evolved a myriad of defense systems to restrict the propagation of phages as well as other parasitic mobile genetic elements such as plasmids. Genetic loci known as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and their associated cas genes serve as one …
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Bacterial defense islands: CRISPR-Cas and toxin-antitoxin systems /
… developed a set of DNA and RNA-targeting systems to combat their invaders – bacteriophages. Fundamental research on these resistance mechanisms led to the discovery of molecular tools for genetic engineering, such as restriction enzymes, bacterial argonautes and CRISPR-Cas systems. …
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Prevention of Duchenne Muscular Dystrophy by CRISPR/Cas Therapeutic Genome Editing
… caused by genetic mutations have a profound and systemic impact on human health. Duchenne muscular dystrophy (DMD) is a lethal neuromuscular disorder, caused by mutations in the DMD gene on the X chromosome, which consists of 79 exons encoding dystrophin protein. Patients with DMD develop …
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Genome analysis of Campylobacter concisus strains from patients with inflammatory bowel disease and gastroenteritis
… 9 GS1-specfic genes and 14 GS2-specific genes. CRISPR-Cas system-associated proteins were only found in some GS2 strains but completely absent from GS1 strains. These results suggest that the two C. concisus genomospecies may differ in their virulence potential and the ability to adapt to …
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Discovery and biochemical characterization of Casl3b, a Type VI-B CRISPR-Associated RNA-Guided RNase
… function or dysfunction in disease. Particular CRISPR-associated (Cas) proteins are used for targeted DNA editing because they are easy to use, precise, and specific. CRISPR-Cas systems function in microbes as adaptive immune systems and are composed of endonuclease protein(s) with targeting …
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Nanopore-based Protein Sensing with DNA Nanostructures
… Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-Associated (Cas) protein system is sequence-specific RNA-guided protein duo that enables binding to a specific nucleic-acid sequence and consequent binding followed by cleavage. The CRISPR-Cas system has recently emerged as a …
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Nuclear Localization of Proteins and Genome Editing in the Oomycete Phytophthora sojae
… losses in aquaculture and fisheries, and occasionally causing dangerous infections of humans. Phytophthora species, represented by the Irish Potato Famine pathogen P. infestans and the soybean pathogen P. sojae, are arguably the most destructive pathogens of dicotyledonous plants among the …
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Investigation of Diverse CARF Effectors in Type III CRISPR - Cas Immunity
… One such example is an adaptive immune system encoded in Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) loci. DNA repeats are separated by short sequences, called spacers, which are acquired from invading genetic elements and incorporated into the CRISPR array by …
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