Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 32 for “"CRISPR-Cas Systems"”.
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Accessory Nucleases Provide Robust Antiparasite Immunity for Type III CRISPR-Cas Systems
… employ diverse and sophisticated defence systems. Clustered, regularly interspaced short palindromic repeats (CRISPR)-Cas systems are adaptive immune systems that can integrate short "spacers" from a parasite into its CRISPR locus as a form of immunological memory. Upon reinfection, short …
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Investigating Genetic (IN)Compatibility Between Temperate Phages and CRISPR-CAS Systems in Staphylococcus Aureus
… form of defense comprises the adaptive immune systems derived from clustered, regularly interspaced, short palindromic repeat (CRISPR) loci and CRISPR-associated (cas) genes. CRISPR-Cas immune systems enable the acquisition of heritable resistance to specific mobile genetic elements on the …
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Restriction-Modification and CRISPR-Cas Systems: Cooperation Between Innate and Adaptive Immunity in Prokaryotes
… and kill them. Restriction-modification (RM) systems represent one such strategy. Generally, these systems provide defense by coordinating the activities of two distinct enzymes: a restriction endonuclease and a methyltransferase. Both enzymes recognize the same short DNA sequences. The …
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PART I CRYSTAL STRUCTURE OF A DIMERIZATION DOMAIN OF DROSOPHILA CAPRIN. PART II CHARACTERIZATION OF TWO CAS13B CRISPR-CAS SYSTEMS FROM PORPHYROMONAS GINGIVALIS
… II: CHARACTERIZATION OF TWO TYPE VI-B CRESPR SYSTEMS: PGI5CAS1B AND PGI8CAS13B WHICH EFFECTOR PROTEINS ARE CAPABLE OF PROCESSING PRE-CRRNA INTO MATURE CRRNA CRISPR-Cas adaptive immune system protects microorganism from foreign nucleic acids invasion through endonucleases activity guided by …
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A tripartite survey of CRISPR-Cas diversity: Assessing the contours and limits of natural variation in CRISPR-Cas systems by associated genes and spacer acquisition biases
Explorations of anti-phage defence systems, with a focus on CRISPR-Cas system diversity, in the past decade has enabled many significant advances in biotechnology especially in programmable gene editing platforms. Many previous investigations have employed guilt by association based data mining …
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A tripartite survey of CRISPR-Cas diversity: Assessing the contours and limits of natural variation in CRISPR-Cas systems by associated genes and spacer acquisition biases
Explorations of anti-phage defence systems, with a focus on CRISPR-Cas system diversity, in the past decade has enabled many significant advances in biotechnology especially in programmable gene editing platforms. Many previous investigations have employed guilt by association based data mining …
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Modulation of the CRISPR-Cas9 Immune Response
… bacteria have developed numerous immune systems to protect themselves, including CRISPR-Cas. CRISPR-Cas systems provide adaptive immunity through the acquisition of short DNA sequences from invading phages, known as spacers. Spacers are inserted into the CRISPR locus and serve as …
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Anti-CRISPRs and CRISPR-Cas: Characterization and Biotechnology
… phage adsorption, restriction modification systems, and the more recently discovered CRISPR-Cas immune systems. Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) loci together with their accompanying CRISPR-associated (Cas) genes form the only known bacterial adaptive defense …
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Discovery and biochemical characterization of Casl3b, a Type VI-B CRISPR-Associated RNA-Guided RNase
… function or dysfunction in disease. Particular CRISPR-associated (Cas) proteins are used for targeted DNA editing because they are easy to use, precise, and specific. CRISPR-Cas systems function in microbes as adaptive immune systems and are composed of endonuclease protein(s) with targeting …
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Bacterial defense islands: CRISPR-Cas and toxin-antitoxin systems /
… developed a set of DNA and RNA-targeting systems to combat their invaders – bacteriophages. Fundamental research on these resistance mechanisms led to the discovery of molecular tools for genetic engineering, such as restriction enzymes, bacterial argonautes and CRISPR-Cas systems. …
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Mechanistic studies of Fusobacterium genetic and defense systems
… exploits innate and adaptive defense systems present in Fusobacterium for their use as molecular tools for genome editing. Accordingly, we first investigated restriction-modification systems with a focus on the role of DNA methyltransferases and endonucleases in host defense and …
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Uncovering New Mechanisms of Type III CRISPR-Cas System Inhibition and Effector Function
… is the bacterial adaptive immune system called CRISPR-Cas, which consists of clustered, regularly interspaced short palindromic repeats (CRISPRs) and a set of genes that encode CRISPR-associated (Cas) proteins. While there are seven distinct types of CRISPR-Cas systems identified thus far, we …
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CPISPR-CAS: From a Prokaryotic Immune System to a Gene Editing Tool
… Regularly Interspaced Short Palindromic Repeats (CRISPR) and their associated genes (cas) encode an adaptive, small-RNA-based immune system that protects prokaryotes from infectious phages and plasmids. CRISPR-Cas systems can be classified into three types based on their cas gene content. My …
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Genetic and Computational Tools for Engineering Complex Prokaryotic Systems
Prokaryotic systems are promising platforms for bioproduction, but the complexity of their gene regulatory and metabolic networks presents significant challenges for engineering efficient biosynthetic pathways. While CRISPR-Cas technologies have revolutionized genome editing in eukaryotes, the …
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Discovery and characterization of Cas13b, a differentially regulated RNA-targeting CRISPR system
… to target RNA in a simple, efficient manner. CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) systems, which confer adaptive immunity to prokaryotes, have revolutionized DNA targeting through the engineering of RNA-programmable Cas9-based …
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Identification and Characterization of Novel CRISPR-Cas Immune Effectors
… this threat. One of the most abundant immune systems bacteria have evolved to combat this are CRISPR-Cas systems, which have been studied extensively due to their fascinating biology and usefulness in biotechnology and therapeutics. My thesis focuses on the discovery and characterization of …
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Discovery of novel systems for genome editing
… questions. The experimental validation of Cas9-based programmable DNA cleavage and its early capacity for targeted genome modification in living eukaryotic cells sparked rapid advancements in CRISPR–Cas genome editing tools. Cas9, when used on its own for genome editing, primarily induces …
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A eukaryotic platform for the enhancement of Cas nucleases
A large repertoire of CRISPR-Cas systems from bacteria, archaea and phages is available for a large variety of genome editing applications. Nevertheless, a limited number of orthologs have the same editing efficiency as SpCas9 in mammalian cells. Moreover, Cas with reduced molecular size that have …
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Novel Cas orthologs from natural reservoirs and design of a directed evolution approach in mammalian cells to enhance their editing potential
CRISPR-Cas systems include several Cas proteins, each with their own enzymatic features and targeting abilities, holding great potential for genome engineering and gene therapy applications. Screenings of metagenomic datasets are continuously providing new additions to the ever-growing CRISPR …
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The role of a toxin-antitoxin system in the arms race between bacteria and phage
… elements, including restriction modification and CRISPR-Cas systems. These defense mechanisms, in turn, have influenced the emergence of phage anti-defense systems, highlighting the intense, ongoing evolutionary arms race between phages and their bacterial hosts. Toxin-antitoxin (TA) systems are …
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