Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 102 for “"CRISPR-Cas"”.
-
Quantitative modelling of CRISPR-Cas editing outcomes
Development of the CRISPR-Cas toolkit over the last decade has enabled unprecedented control over the genome and unlocked the capacity for new experiments and gene-therapies. However, use of these technologies is made more difficult due to variance in the rate and type of individual genetic …
-
Anti-CRISPRs and CRISPR-Cas: Characterization and Biotechnology
… systems, and the more recently discovered CRISPR-Cas immune systems. Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) loci together with their accompanying CRISPR-associated (Cas) genes form the only known bacterial adaptive defense mechanism that effectively protects …
-
Bacterial defense islands: CRISPR-Cas and toxin-antitoxin systems /
… as restriction enzymes, bacterial argonautes and CRISPR-Cas systems. CRISPR-Cas systems are located in the genome part called “defense islands” where genes encoding different antiviral systems are clustered together. The goal of this thesis is to explore the mechanisms of action of type I …
-
Identification and Characterization of Novel CRISPR-Cas Immune Effectors
… systems bacteria have evolved to combat this are CRISPR-Cas systems, which have been studied extensively due to their fascinating biology and usefulness in biotechnology and therapeutics. My thesis focuses on the discovery and characterization of three new CRISPR-Cas effector proteins that provide …
-
Cas9-Primed Adaptive Immunity During the CRISPR-Cas Response
… regularly interspaced short palindromic repeats (CRISPR) loci provide archaea and bacteria with adaptive immune systems that allow them to counteract these rapidly evolving genetic parasites. These diverse systems all generally contain two components: a set of CRISPR-associated (cas) genes and a …
-
An integrated CRISPR-Cas toolkit for engineering human cells
… Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated (Cas) system is a prokaryotic adaptive immune system that confers resistance to foreign genetic elements including plasmids and phages. Very recently, a two-component CRISPR-Cas technology from Streptococcus Pyogenes comprising of …
-
Interrogation of CRISPR-Cas targeting specificity for mammalian genome engineering
Class II CRISPR-Cas RNA programmable DNA endonucleases enable high efficiency genome editing across the biological diversity for research, industrial, and biomedical applications. Human genome editing with CRISPR-Cas just recently made its debut in human clinical trials and has immense therapeutic …
-
A tripartite survey of CRISPR-Cas diversity: Assessing the contours and limits of natural variation in CRISPR-Cas systems by associated genes and spacer acquisition biases
… of anti-phage defence systems, with a focus on CRISPR-Cas system diversity, in the past decade has enabled many significant advances in biotechnology especially in programmable gene editing platforms. Many previous investigations have employed guilt by association based data mining approaches to …
-
A tripartite survey of CRISPR-Cas diversity: Assessing the contours and limits of natural variation in CRISPR-Cas systems by associated genes and spacer acquisition biases
… of anti-phage defence systems, with a focus on CRISPR-Cas system diversity, in the past decade has enabled many significant advances in biotechnology especially in programmable gene editing platforms. Many previous investigations have employed guilt by association based data mining approaches to …
-
Prevention of Duchenne Muscular Dystrophy by CRISPR/Cas Therapeutic Genome Editing
… treatment for this debilitating disease. The CRISPR/Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) was first discovered as an adaptive immune system in bacteria and archaea for defending against phage infection. Recently, the CRISPR/Cas system …
-
Investigation of Diverse CARF Effectors in Type III CRISPR - Cas Immunity
… Regularly Interspaced Short Palindromic Repeats (CRISPR) loci. DNA repeats are separated by short sequences, called spacers, which are acquired from invading genetic elements and incorporated into the CRISPR array by CRISPR-associated (Cas) proteins Cas1 and Cas2.The CRISPR locus also encodes …
-
Functional Organization of Molecular Memories in the CRISPR-Cas Immune System
<p>CRISPR-Cas systems endow bacteria and archaea with adaptive immunity against foreign genetic threats, like phages and plasmids. These immune systems are comprised of CRISPR-associated (Cas) protein effectors and DNA-based storage of immunological memories in the CRISPR array. The CRISPR array is …
-
Advancing plant transformation and CRISPR/Cas editing: Strategies for recalcitrant maize
… In addition, the selection of an efficient CRISPR/Cas based gene editing system and target sequence becomes imperative prior to stable genetic transformation, particularly when dealing with recalcitrant plant species. Comprehending the factors that impact genetic transformation and …
-
Accessory Nucleases Provide Robust Antiparasite Immunity for Type III CRISPR-Cas Systems
… regularly interspaced short palindromic repeats (CRISPR)-Cas systems are adaptive immune systems that can integrate short "spacers" from a parasite into its CRISPR locus as a form of immunological memory. Upon reinfection, short RNAs transcribed from the CRISPR locus can guide Cas proteins to the …
-
Roles of non-Cas nucleases in Type III-A CRISPR-Cas immunity
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-08-01
-
DEVELOPMENT OF CRISPR-CAS IMMOBILIZATION SRATEGIES FOR THE FABRICATION OF A BIOSENSING PLATFORM
… strategies for the immobilization of the CRISPR-Cas enzyme, to create an integrated platform for the detection of DNA samples. In recent years, the CRISPR-Cas based diagnostic is emerging as a promising technology for the detection of infectious disease in point of care (POC) environments …
-
Uncovering New Mechanisms of Type III CRISPR-Cas System Inhibition and Effector Function
… is the bacterial adaptive immune system called CRISPR-Cas, which consists of clustered, regularly interspaced short palindromic repeats (CRISPRs) and a set of genes that encode CRISPR-associated (Cas) proteins. While there are seven distinct types of CRISPR-Cas systems identified thus far, we …
-
Detección de un polimorfismo marcador de sexo mediante CRISPR-CAS 12A en salmón coho
… requieren de equipos especializados y en algunos casos son muy laboriosas de implementar en la práctica. El sistema CRISPR-Cas permite la detección de nucleótidos utilizando el mecanismo de acción de la enzima Cas 12a, que reconoce una secuencia de ADN específica dirigido por un ARN guía, la cual …
Page 1 of 6