Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 11 of 11 for “"CRISPR technology"”.
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Development of a novel screening platform for identifying genetic pathways regulating axon regeneration
… disease mutations. Due to its many advantages, CRISPR technology is an attractive tool to irreversibly remove the gene of interest by targeting its DNA. The overall goal is to identify negative regulators in axon regeneration. Here, we performed candidatebased studies to assess novel candidates …
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Regulation of mammalian IRE1α: Co-chaperones and their importance
… UPR. This thesis also utilises the power of Cas9-CRISPR technology to introduce specific mutations into the endogenous IRE1α locus and to screen for derepressing IRE1α mutations. Via this methodology, two predicted unstructured regions of IRE1 are found to be important for IRE1 repression. …
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Computational and experimental methods for CRISPR-based saturation mutagenesis screens
… of the human genome. The emergence of CRISPR technology has enabled the efficient and diverse installation of genetic variation in situ, leading to its widespread use in functional genomics. The application of high-throughput CRISPR saturation mutagenesis screens for the functional …
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Exploiting Chemogenetic and Genetic Interactions In Human Cells As An Avenue For New Therapeutic Opportunities
<p>The advent of CRISPR technology and its adaptation to the mammalian genome made whole-genome knockout screens possible directly in human cells. Gene knockout answers how essential that gene is for cell fitness and proliferation. Genes showing moderate to severe fitness defects are called …
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Identifying regulators of cytotoxic T cell function through molecular and genetic screening
… In this study, I aimed to establish the CRISPR-Cas9 gene editing technology in primary T cells and to optimise screening approaches to identify regulators of CTL killing. The first half of the thesis focuses on primary mouse CTL. The CRISPR technology was successfully optimised in CTL …
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The Role of the Proneural Transcription Factor ASCL1 in Neuroblastoma Cell Division and Differentiation
… ASCL1 deletion on neuroblastoma cell behaviour. CRISPR technology was used to remove ASCL1 from three different neuroblastoma cell lines to study the effect of losing ASCL1 in different cellular contexts. It was found that ASCL1 deletion results in slower cell growth, a phenotype consistent in …
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Studying the Interaction of RACK-1 and GLD-1 in the Germline of Caenorhabditis elegans
… and GLD-1. RACK-1 strains were tagged using CRISPR technology. These strains were shown to behave similarly to a wildtype organism through immunostaining and brood size assay experiments. As a result, the strains were used as reagents for co-immunoprecipitation experiments to detect an …
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A broadened HLA ligandome uncovers new immunotherapy targets for pancreatic cancer + A prime editor mouse to model a broad spectrum of somatic mutations
… challenging or infeasible to achieve with other CRISPR technology. We demonstrate the utility of this system by mediating secondary Kras mutations and common Trp53 hotspot mutations in model-derived pancreatic organoids. Finally, we model lung and pancreatic cancer in vivo using lentiviral …
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Understanding functional mechanisms of genetic susceptibility to mycobacterial infection
… projects. In my first project, I used RNAi and CRISPR-Cas9 technologies to study the role of ASAP1 in dendritic cells and macrophages, cells that play critical roles during mycobacterial infection. I demonstrated that in these cells ASAP1 is essential for migration and phagocytosis of …
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Myxoid Liposarcoma: Models and Mechanisms of Sarcomagenesis and Response to Radiation Therapy
… We also employ in vivo electroporation and CRISPR technology to rapidly generate spatially and temporally restricted mouse models and cell lines of high grade FUS-CHOP-driven sarcomas for preclinical studies.</p>
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Using human genomics to decipher biological mechanisms underlying reproductive ageing and fertility in women
… human genomic evidence with cutting edge CRISPR technology and the In vitro gametogenesis system, in Chapter 7 I investigate the role of PARP-1 in proliferation of primordial germ cells during the establishment of the ovarian reserve. I demonstrate suggestive evidence on the role of PARP-1 …