Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 4 of 4 for “"CRISPR/Cas9 genetic screen"”.
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CRAMP1 drives linker histone expression to enable Polycomb repression
… mammalian genomes (11 in mice and humans) hamper genetic studies to determine H1 function in vivo. Challenging the prevailing view that linker histones are a general feature of heterochromatin, here I show a selective requirement for linker histones in the function of Polycomb Repressive complex 2 …
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Genome-wide search for regulators of the first cell lineage decision in the mouse: a CRISPR/Cas9 endeavour
… to trophoblast in an unbiased approach, using CRISPR/Cas9 genome-wide loss of function screening. I developed a reporter cell line that constitutively expressed Cas9 from the Rosa26 locus, and was engineered with knock-in of T2A-Venus into the Elf5 locus (Elf5::Venus ESCs). Elf5 is a stringent …
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Characterization of endocytic escape pathway and its role in adaptive immunity
… of proteins, the Kozik lab has established a genetic screening strategy where escape of a ribosome-inactivating protein, called saporin, is being followed. When saporin enters the cytosol, it causes translational arrest quantified using puromycin labelling of newly synthesized polypeptides. …
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Focused CRISPR-Cas9 screens investigating the DNA damage response
… syndromes to developmental disorders. Genetic screens can be used to gain insights into the function of known DDR proteins, identify novel DDR components and highlight potential therapeutic opportunities. We demonstrate that CRISPR-Cas9 gene editing is a useful tool in genetic screens …