Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"CRISPR/Cas12a"”.
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Methods for CRISPR Cas12a Multiplexing in Mammalian Systems
… regularly interspaced short palindromic repeat (CRISPR) proteins have been found in many bacterial species, serving as a defense mechanism to defend the cell from bacteriophage invasion. These proteins are guided to cleave a specific nucleic acid sequence through the binding of a crRNA hairpin. …
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The IN4MER CRISPR/Cas12a Multiplex Knockout Platform and Its Applications
… In this study, we conducted a meta-analysis of CRISPR genetic interaction screens. We identified a candidate set of synthetic lethals that are independent of background, and showed that the Cas12a platform exhibited enhanced sensitivity and consistency. Building on this knowledge, we developed a …
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Development of Universal Biosensing Platforms Based on CRISPR/Cas12a systems
CRISPR/Cas technologies possess the promising potential to affect biosensing field by providing a sensitive, precise, rapid, versatile and cost-effective method for diverse target detections. This thesis focusses on the development of CRISP/Cas12a based biosensing platforms for nucleic acid and …
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Utilizing the IN4MER CRISPR/CAS12A Multiplex Knockout Platform To Investigate Synthetic Lethality in the Human Genome
… candidate synthetic lethal paralogs; 2) our Cas12a multiplex platform “IN4MER” that provides superior sensitivity and replicability to support genome- wide and genetic interaction screening; 3) observations and analyses to account for varied guide efficiency in multiplex CRISPR screen …
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Development of a Multi-Component Biosensor for Recombinase Polymerase Amplification-CRISPR/Cas12a-Based Detection of Fusarium oxysporum f. sp. cubense tropical race 4
… Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas12a-mediated detection, and electrochemical square wave voltammetry (SWV) with targeted integration into a microfluidic system for amplification and detection within a compact heating unit. The research was organized into several key …
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Towards Accessible Diagnostics: Developing CRISPR-Based Colorimetric Tools for Low- and Middle-Income Countries
… disease burdens. This project aims to adapt CRISPR-Cas12 technology into a robust, easily manufacturable, and virtually equipment-independent diagnostic test suitable for low- and middle-income countries. Enteric fever detection is explored given its highly contagious nature and prevalence of …
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CRISPR-integrated Biosensors for Human Health: Novel strategies for pathogen detection and cancer screening
… clinical decisions, and ensuring food safety. CRISPR-based biosensors offer a promising solution due to their programmability, sensitivity, and versatility. This dissertation focuses on the development of CRISPR-integrated biosensing platforms for assessing antimicrobial resistance, detecting …
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Signal Processing Techniques Applied to Biomedical Diagnostics
… that pair this technology with isothermal CRISPR-Cas12a-based DNA/RNA amplification to detect clinically relevant targets with high specificity. Finally, we evaluate the clinical performance of our systems in detecting target genes for (1) SARS-CoV-2, the virus responsible for the COVID-19 …
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NANOGP1 as a Model to Study the Consequences of Gene Duplications on Human Pluripotency and Development
… to distinguish between the two genes. Using CRISPR/Cas12a-mediated gene editing in naïve PSCs, I introduced an epitope tag at the start of the predicted protein sequence, and this enabled me to demonstrate for the first time that endogenous NANOGP1 encodes an expressed protein. The ability to …
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Engineering programmable nucleases for in vivo and in vitro applications
… regularly interspaced short palindromic repeats (CRISPR) associated nucleases, and Argonaute nucleases (Ago) have revolutionized genome engineering by allowing precise and easy manipulation of genetic material. These reprogrammable nucleases hold immense potential not only for genome editing but …
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Multiplexed Crispr Libraries For Cancer Functional Genomics
… with disease contexts. The adaptation of CRISPR/Cas9 has improved the sensitivity and specificity of functional screenings. Despite this advance, there remains a long-standing need to improve functional screenings with smaller and more versatile pooled libraries. Capitalizing on the …