Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 36 for “"CPPs"”.
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Peptide conjugation to enhance oligonucleotide delivery
… of interest to a cell-penetrating peptide (CPP). CPPs are widely employed to enhance macromolecule delivery, with hundreds of different peptides and modifications reported to improve cellular uptake. In this thesis, CPPs were systematically investigated and chemically altered to facilitate the …
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Development of a Real-Time Cell Imaging Assay to Characterize the Delivery of a Cell-Penetrating Peptide Adaptor and its Associated Cargo
<p>Cell-penetrating peptides (CPPs) are small peptides capable of transporting molecules across the membrane. In most cases, the membrane is a blockade that prevents molecules from entering the cell, therefore CPPs have attracted much attention. Although CPPs are effective, this system is still …
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Cambios en los parámetros vocales de docentes, durante el curso escolar, medidos con PRAAT e Índice Acústico de Calidad Vocal
… acústico de disfonía (AVQI), la calidad de voz (CPPS) y el ruido vocal (HNR) con el programa de análisis acústico PRAAT. Además, se estudió la percepción que tenían los docentes sobre su salud vocal con el test VHI-10 versión española. La muestra se compuso de 93 docentes de los cuales el 71% …
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From Act to Action: Normalizing Child Protection Policies in an Aotearoa New Zealand School
… 2014 legislated for child protection policies (CPPs) to be implemented in all schools, in place of mandatory reporting. This includes the expectation all personnel—not just teachers, the usual focus of literature on CAN training—be able to identify and respond to both suspected and actual abuse …
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Utilization of the Cell Penetrating Peptide TAT-CaM in New Biological Systems: Fungal and Bacterial Cell Lines
<p>Cell penetrating peptides (CPPs) are biomolecular vehicles or systems that mediate the delivery of proteins into different cell lines. Since their discovery in the late 1980s, the CPP delivery system has primarily been explored in different types of mammalian cells. The technology has …
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Peptide-mediated delivery of antisense oligonucleotides and chemotherapeutics across biological barriers
… of macromolecules. Cell-penetrating peptides (CPPs) that interact with membranes can improve the uptake of macromolecules across biological barriers. Here we explore methods for the peptide-mediated delivery of antisense oligonucleotides (ASOs) and chemotherapeutics. First, we address the issue …
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Bioengineering of a TAT-conjugated Peptide to Modulate the Activity of Glycogen Synthase Kinase-3 in Adult and Embryonic Stem Cells
… or proteins using cell-permeable peptides (CPPs) which have the ability of crossing the plasma membrane and carrying cargos into cells. CPPs can therefore be used to deliver factors to direct stem cells proliferation, survival and differentiation. This thesis describes an approach to control …
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Simultaneous Delivery of CRISPR/Cas Complexes and Donor DNA Using Cell-Penetrating Peptide-Adaptors
… (CPP)-adaptor system. Since the discovery of CPPs in the 1980s, researchers have used CPPs to deliver biomolecules such as proteins and nucleic acids into cells (the delivered biomolecules are called cargos). Researchers encountered the problem of endosomal entrapment: the CPP-cargos remained …
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Design of Nuclear-Targeting Peptides for Macromolecule Delivery via Machine Learning
… For example, cell-penetrating peptides (CPPs) are capable of delivering macromolecular cargo to intracellular targets that are otherwise inaccessible. However, design of novel CPPs with high activity and unique structure remains challenging. In this thesis, methods to design and …
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Improvement of cleaning effectiveness through Statistical Process Control in active pharmaceutical ingredient (API) manufacturing
… steps, identifying critical process parameters (CPPs) and calculation of control limits using Shewhart Control Charts for these CPPs. Significant modifications were done to the automation controls for the recipe to ensure deviations from recipe are captured and appropriate actions are taken by …
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The Discovery and Chemical Synthesis of Peptides and Proteins that Cross Biological Barriers
… a complex task. Cell-penetrating peptides (CPPs) and proteins are used as carrier modules, tissue targeting agents, and probes to better understand disease states. In this work, we first investigate CPPs for peptide-mediated delivery of macromolecules. Despite the existence of thousands of …
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REGULATED UPTAKE OF BIOPOLYMERS Role of cell surface proteoglycans Implications for drug and gene delivery
… field of so called cell penetrating peptides (CPPs). Due to their ability to efficiently deliver macromolecular cargo over the plasma membrane, CPPs have proven to be useful tools in basic research. More importantly, the technology has been shown to enhance delivery of a number of …
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Predictive Disintegration Process Modelling of Immediate Release Tablets Using Terahertz Technology
… (CMAs) and Critical Process Parameters (CPPs) to Critical Quality Attributes (CQAs) like disintegration performance is essential, particularly during scale-up. Predicting disintegration for immediate-release tablets remains challenging due to the process complexity. This research …
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Integration of quality-by-design into control systems design for continuous pharmaceutical manufacturing
… spaces: sets of critical process parameters (CPPs) which guarantee that critical quality attributes (CQAs) of a manufacturing process are within specifications. For continuous pharmaceutical processes, design spaces are usually calculated by assuming steady state operation and approximating …
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Design, Synthesis And Characterization Of Lipidated Pna-Peptide Conjugates As Potentialtherapeutic And Diagnostic Reagents
… After solid-phase automated synthesis, PNA-CPPs: TAT/Arg9) were coupled with lipids or phospholipids before cleavage from the support and then purified by HPLC. The conjugates were characterized by UV-vis and MALDI, and by their ability to form vesicles in water and to partition between …
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Intervención de terapia manual osteopática en hombres con síndrome de dolor pélvico crónico o prostatitis crónica.
… paciente. Las clasificación más aceptada de la CPPS ha sido la propuesta por el NIDDK (National Institute of Diabetes and Digestive and Kidney Diseases) en función de la duración de los síntomas, y la prueba de orina de cuatro vaos de Stamey. En el presente trabajo se incluye la prostatitis de …
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Novel cell-penetrating peptide-based vectors for siRNA delivery
… as a therapeutic. Cell penetrating peptides (CPPs), such as the Antennapedia homeodomain (AntpHD) and its third helix, Penetratin, are well characterised cationic motifs used previously to deliver covalently linked nucleic acid cargo, such as siRNA in vitro and in vivo, may offer a strategy to …
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The California energy crisis and cogeneration investment opportunities for office landlords
… placed on California's centralized power plants (CPPs). Reducing the demand for electricity that is centrally generated and delivered via transmission lines reduces the risk of blackouts and the cost of wholesale and retail electricity. Second, because transmission lines are already operating at …
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Transcending the Cell Fate Stereotype: Cell Penetrating Peptide Technology helps Induce Pluripotency in Multiple Cell Types
… factor proteins via cell penetrating peptides (CPPs) overcomes these issues, however, endosomal entrapment commonly results in inefficient cargo delivery. These reprogramming protocols produce inefficient results that lack the propensity to be translated into direct patient therapy, ultimately …
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Synthesis, Characterization, and Evaluation of Amphiphilic Cell-Penetrating Peptides as siRNA Delivery Tools for Cancer Therapy
… fatty acyl cell-penetrating peptides (CPPs) as siRNA delivery tools. We hypothesized that conjugation of hydrophobic molecules to the (HR)n peptide will enhance hydrophobicity, modulate cytotoxicity, and provide an efficient delivery system for siRNA. Therefore, we synthesized peptide …
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