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Showing 1 to 13 of 13 for “"COS7"”.
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Identifizierung regulatorischer Proteindomänen der zytolytischen Eigenschaften des humanen P2X7-Rezeptors
… valdidate the developed method, I investigated COS7-cells expressing the wildtype-P2X7-cDNA and 264.7-RAW-macrophages which expresses an endogenous P2X7-receptor. Furthermore I investigated the previous published E496A-Polymorphismem. According to the literature the activation of transfected …
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Identifikation und Charakterisierung von Interaktionspartnern des Zystennierenproteins DZIP1L
… studies in transiently transfected COS7-, HEK293- and mIMCD-3- cells have been performed for validation of the interaction of DZIP1L with EEF1G, NAGK and PSAP. All three identified binding partners are involved in SMAD-/TGF-ß- signaling. NAGK and PSAP are known to directly interact …
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QUANTIFYING OLIGOMERIC STATES OF PROTEINS IN-CELL VIA SINGLE-MOLECULE SUPER-RESOLUTION MICROSCOPY
… PA-JF549, and acceptor fluorophore, JF646, in COS7 cells. Analyzing the FRET contribution on donor's intensity distributions allows us to estimate the dimerization of SOD1 proteins in cells. To apply our methods to a system that can provide physiologically relevant information, we further used …
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Charakterisierung fluoreszierender Varianten des Zytokinrezeptors gp130 hinsichtlich biologischer Aktivität und Dimerisierung mit molekularbiologischen und fluoreszenzspektroskopischen Methoden
… has been constructed. After expressing in COS7-cells its behaviour has been analysed during Laser-scanning Microscopy and Fluorescence Spectroscopy.
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On the neuroendocrine regulation of reproduction: Functional characterization and kinetic studies of the lamprey gonadotropin -releasing hormone receptor and cloning and analysis of the cDNA encoding lamprey gonadotropin-releasing hormone-III
… expression system was developed using COS7 cells transiently transfected with the lamprey GnRH receptor. The lamprey GnRH receptor was shown to be functional as well as lamprey GnRH-III selective based on a series of efficacy and kinetic studies. Ligand dependant internalization was …
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Lamin A and lamin C are differentially dysfunctional in autosomal dominant Emery-Dreifuss muscular dystrophy
… polymorphism (SNP) in transfection studies in COS7 fibroblasts and, partially, in C2C12 myoblasts. The EGFP or DsRed2 tagged lamins were exogenously expressed either individually or both A-types together and examined by light and electron microscopy. The protein mobility of lamin A mutants was …
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An Alternative View of the Templates and Functions of RNA Polymerase II
… RNA also modulates HDV replication in vivo, in COS7 and HeLa cells. The correlation between the effects of secondary structure alterations on the efficiency of pol II transcription in vitro and HDV replication in vivo suggests that the observed RNA-templated pol II transcription in vitro …
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Pathologie und Pathogenität eines viralen Strukturproteins : Faltung und pathogene Effekte von mutierten TMV -Hüllproteinen in pflanzlichen und tierischen Zellen
… transient in Säugerzellen (Neuro2a und Cos7) transfiziert und die Zustandsformen der TMV-HPs und die Reaktion der Zelle darauf untersucht. Die Bedeutung der Prolinreste (P) im TMV-HP für seine Stabilität wurde durch den Austausch von P gegen Leucin (PnL) an den acht möglichen Positionen …
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Morphological Remodeling of the Endoplasmic Reticulum During Mitosis and Its Influence on the Integrity of the Spindle Apparatus
… subclasses we overexpressed each fly Reep in Cos7 cells, resulting in varied phenotypes with ReepB appearing to remodel ER tubules more readily. We then turn back to fly embryos to elucidate the mechanism behind <em>ReepB</em> spindle disruption. Together these data suggest that ReepA and …
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Microelectrode Array Modeling of Genetic Neurological Disorders in the Era of Next Generation Sequencing
… We first investigate ATP1A3 mutations in COS7 cells and observe no clear differences. We next evaluate the effect of two mutations that cause the most severe ATP1A3-associated disorder, Alternating Hemiplegia of Childhood (AHC), on network dynamics. We show that mutant cultures demonstrate …
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Untersuchung zu (epi)genetischen Veränderungen auf Chromosom 11p15 und ihre funktionelle Relevanz bei Patienten mit Silver-Russell-Syndrom
… out expression analyses in cultured HEK293- and Cos7-cells after transfection with constructs containing the different variants. An altered splicing of the H19 mRNA in comparison to wild-type construct could be found in two of the three patients (SR81K; SR93K), therefore indicating a relevance of …
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Molecular and Biochemical Investigations into VMD2, the gene associated with Best Disease
… These included the establishment of an in vitro COS7 heterologous expression assay, the generation of numerous VMD2 mutations by site-directed mutagenesis as well as the development of bestrophin-specific antibodies. Surprisingly, membrane fractionation/Western blot experiments revealed no …
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Funktion des Wind-Proteins in Drosophila melanogaster bei Faltung und/ oder Transport des sekretorischen Proteins Pipe
Wind ist ein ER luminales Protein der Protein Disulfid Isomerase-Familie, welches seine Bedeutung in der Dorsoventral-Entwicklung von Drosophila hat. Es ist für die korrekte Lokalisation eines weiteren Proteins Pipe, einem Golgi-Transmembranprotein, verantwortlich. In Zusammenarbeit mit der Abt. f. …