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Showing 1 to 20 of 29 for “"COPII"”.
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P125, A COPII INTERACTING PROTEIN
Coat protein complex II (COPII) coated vesicles/carriers mediate the export of proteins from the ER via specialized domains known as the ER exit sites (ERES). p125A is a protein of 125 kDa that interacts with Sec23A, a component of the COPII coat. It was found to participate in the organization of …
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Principles of COPII Coat Assembly
Protein secretion in eukaryotic cells begins with COPII-mediated transport from the endoplasmic reticulum (ER). The COPII coat assembles on the cytoplasmic side of the ER membrane in consecutive stages. The inner coat comprises the Sar1-Sec23/Sec24 heterotrimeric complex and is recruited through …
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Proteins that interact with Sec16p during COPII vesicle formation in Saccharomyces cerevisiae
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1996.
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Towards an understanding of the role of COPII subunits in vesicle coat assembly
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1997.
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Regulation of COPII vesicle formation and protein sorting by the BST genes in Saccharomyces cerevisiae
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1998.
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Role of Sec16p in the formation of COPII-coated ER to Goldi transport vesicles in Saccharomyces cerevisiae
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1998.
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From the Endoplasmic Reticulum to the Golgi Apparatus : In Vitro and In Vivo Approaches to Understanding COPII Vesicle Function in Plant Cells
… Reticulum (ER) to Golgi transport is mediated by COPII vesicle in yeast and mammalian cell. COPII coats consist of the small GTPase Sar1p and the heterodimeric protein complexes Sec23/24 and Sec13/31. COPII mediated sorting occur when protein cargoes exit the ER. Although the principles of …
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Lst1p and Exp1p act in parallel pathways to export the plasma membrane H⁺-ATPase from the ER in S. cerevisiae
… and packaged into vesicles coated with the COPII protein complex. To facilitate our study of the mechanisms of protein sorting, we have selected Pma1p, the plasma membrane H+ ATPase of S. cerevisiae, as a model cargo protein. We found that efficient trafficking of Pma1p to the cell surface …
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Role of O-GlcNAc in the Vertebrate Secretory Pathway
… have shown that several coat protein complex II (COPII) components including SEC23A, SEC24C, and SEC31A are O-GlcNAcylated. The COPII coat complex consists of protein coated carriers that mediate secretory trafficking from the endoplasmic reticulum. To determine the effects of O-GlcNAc on COPII we …
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Luminal calcium regulates membrane fusion in the early secretory pathway
… effect on homotypic coat protein complex (COPII) vesicle fusion. In particular, chelation of luminal calcium as well as fast chelation of extra luminal calcium, result in a significant increase of COPII vesicle fusion. Specific inhibition of Sarco/endoplasmic reticulum calcium ATPases using …
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Luminal calcium regulates membrane fusion in the early secretory pathway
… effect on homotypic coat protein complex (COPII) vesicle fusion. In particular, chelation of luminal calcium as well as fast chelation of extra luminal calcium, result in a significant increase of COPII vesicle fusion. Specific inhibition of Sarco/endoplasmic reticulum calcium ATPases using …
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Molecular Mechanisms of SURF4- mediated Protein Secretion
… transport carriers that are generated by the COPII coat. Efficient and accurate cargo incorporation into ER-derived carriers is driven by transmembrane cargo receptors. ER export receptors are especially important for secretion of soluble cargo as they provide a transmembrane bridge to the …
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Structural studies of large architectural nucleoporins and coat proteins
… an a-helical domain structurally related to the COPII coat protein Sec31. This domain is called the ancestral coatomer element 1 (ACE1). Sec13-Sec31 and Nup84-Nup145C-Sec13 complexes form analogous edge elements for the COPII coat and the NPC. A sequence-based search for other ACE1s identified …
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Defining the Cellular and Molecular Basis of Spondyloepiphyseal dysplasia tarda
… proteins reach the Golgi apparatus through COPII carriers. COPII vesicles bud from sub-domains of the ER, named ER exit sites (ERES) and are composed of five rapidly cycling proteins: the small GTPase Sar1, the inner coat (Sec23/Sec24) and the outer layer (Sec13/31). The efficiency of COPII …
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Cornichon Proteins: Unexpected Roles in Plant Pathogen Infection, ER Morphology Maintenance and Pollen Development
… as a cargo receptor of coat protein complex II (COPII) for protein trafficking from the endoplasmic reticulum (ER) to the Golgi apparatus, en route to their final destinations. By interacting with specific cargo proteins, CNI proteins regulate key steps of embryo polarity in Drosophila, budding …
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Trafficking motifs in potassium channels
… ER exit of the KATP and hERG channels in COPII vesicles. Blocking the cargo binding sites on the Sec24 protein of the COPII coat with membrane-permeable synthetic peptides prevented ER exit of both these channels. While the diacidic 280DLE282 sequence on the Kir6.2 subunit of KATP channels …
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Identifizierung und Charakterisierung der Hüllproteine von pflanzlichen COP Vesikeln
… der Proteinhülle pflanzlicher COPI und COPII Vesikel hergestellt. Mit diesen Antikörpern konnten gamma-COP und Sec23p als Bestandteile der pflanzlichen COP Vesikel charakterisiert werden. Außerdem wurden Immunlokalisierungen mit verschiedenen Antikörpern durchgeführt, die zeigten, dass …
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Identifying the Molecular Cause of Extreme Endoplasmic Reticulum Dilation in Pediatric Osteosarcoma and Its Relationship to the Disease
… exhibit reduced expression of four critical COPII vesicle proteins that has resulted in the accumulation of procollagen-I protein within ‘hallmark’ dilated ER. Using CRISPR activation technology, increased expression of only SAR1A and SEC24D to physiologically normal levels was sufficient to …
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Translational tuning pre-emptively modulates protein folding and secretory pathway defects
… of defects associated with mutations in Sec24, a COPII coat protein that generates ER-derived transport vesicles. Loss of Eap1 and other translation regulators exacerbates various Sec24 growth phenotypes. I further show that translation at the ER is repressed in cells where COPII vesicle formation …
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Characterization of Drosophila Scap: Analysis of Mutants and Evidence for a Retention Factor
… components of the coatamer protein complex II (COPII) when lipid levels fall. In the Golgi, SREBP is cleaved sequentially by two proteases, S1P and S2P. By contrast to mammalian cells, which cannot survive without S2P or Scap, flies lacking Scap or S2P can activate SREBP. These mutants survive …
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