Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"CLOCK Proteins"”.
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Screening for kinases regulating subcellular localization of clock proteins using human kinome library
국문 요약 ⅰ 차례 ⅳ 그림 차례 ⅶ 표 차례 ⅸ Ⅰ. 서론 1 A. 생체시계의 분자 생물학적 기전 1 B. 인산화에 의한 생체시계 조절 2 C. 핵 이동 5 D. 연구의 필요성 5 Ⅱ. 재료 및 방법 6 A. Plasmids and cloning 6 B. Establishment of human kinome expression library 9 C. Cell culture and transfection 9 D. Fluorescence microscopy 11 E. Quantitative real-time RT-PCR 12 F. …
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Investigating the relationship between clock gene stability and the pace of the vertebrate segmentation clock
… with waves of cycling mRNA expression of ‘clock’ genes sweeping across the PSM that are regulated by a molecular oscillator. Mathematical models suggest that sustained rapid oscillations of Notch-regulated gene transcription in the PSM depend on transient negative feedback loops that rely …
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The Gsk-3Β-Fbxl21 Axis Regulates Tcap Via Ubiquitin-Mediated Proteasomal Pathway In The Cytoplasm
… E3 ligase activities targeting the core clock proteins CRYPTOCHROME(CRY)1/2. Here we identified a new sarcomeric target substrate, Telethonin(TCAP), which also shows circadian oscillation in its mRNA transcript and protein expression and, importantly, interaction with FBXL21 in an …
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Genetic noise in the cyanobacterial circadian oscillator
Clocks are generally considered as quintessential examples of accurate and precise devices. Biological clocks however, are continually subjected to intracellular and extracellular fluctuations that might reduce the fidelity of this periodic timer. One fundamental limitation that might set an upper …
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Non-Photic Mechanisms of Entrainment In Bmal1 Deficient Conditions
… our internal circadian (i.e. 24 -hour) clock is imperative to our daily biological and mental well-being. Large epidemiological studies have shown that disruptions of our circadian rhythms can lead to poor mental health, metabolic diseases, and various types of cancer. Various external …
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Splicing The Clock Is Timeless: From Fruit Fly Behavior To Mammalian Retinal Degeneration
… in which the rhythmic expression of core clock proteins negatively regulates their own expression, are a central mechanism of all eukaryotic circadian clocks. How TTFLs maintain separate phases of transcription and negative feedback and generate a ~24 hour period is a key unresolved …
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Circadian desynchrony in the mouse: how does chronic exposure to rotating shift work-like patterns of light/dark affect circadian rhythms and neurobehavioural outcomes
… changes as a result of a round-the-clock activity regime might be. The present study examines male CD-1 mice treated with three different paradigms of rapidly rotating shift work-like light/dark manipulation compared to two control groups maintained on a standard 12:12 h light/dark …
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Circadian Orchestration of the Cellular Proteome
… transcription and translation of a core set of ‘clock proteins’. This cellular timekeeping system is remarkably precise and robust to perturbations. In the first chapter of this thesis I explored the mechanism of action of picrotoxin, a drug that shortens circadian period through unknown means. I …
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The Role of CLOCK in Regulation of Dopamine Neurotransmission in the CLOCKdelta19 Mutant Mouse Model
Mice with a mutation in the circadian gene Clock (Clockdelta19) display a behavioral profile which parallels a euphoric manic-like state including hyperactivity, disrupted activity rhythms, increased substance abuse vulnerability, and decreases in anxiety and depression-related behavior. The …
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Chronic Circadian Misalignment Disrupts the Circadian Clock and Promotes Metabolic Syndrome
… misalignment of an organism’s internal circadian clock with diurnal, cyclic changes in the external environment, prevalent in professions that require shift work, contributes significantly to Type 2 Diabetes development. Experimentally, only short-term models of circadian disruption have been …
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Architecture of the Mammalian Circadian Repressive Complex
… physiology to the solar day. Mammalian circadian clocks are driven by a transcription/translation feedback loop composed of positive regulators (CLOCK/BMAL1) and repressors (CRY1/2 and PER1/2). To understand what drives periodicity within this clock, I took structural approaches with the hope of …
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The Role of Codon Usage in Regulating Protein Expression, Structure and Function
… (FRQ) protein are essential for circadian clock function. However, unlike most genes inNeurospora, frq exhibits non-optimal codon usage across its entire open reading frame (ORF). Optimization of frq codon usage results in the abolition of both overt and molecular circadian rhythms. Codon …