Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"CLIP-seq"”.
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Assembling The Building Blocks For A Unified Splicing Code
… eukaryotes, DNA is transcribed to pre-mRNA. Subsequently, a single pre-mRNA can encode for different proteins by selectively including or excluding protein-coding and non-coding RNA fragments from the final mRNA product through a process called alternative splicing. Alternative splicing is a …
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Understanding The Role of Arglu1 In Interferon Signaling Activation In Breast Cancer
… was expressed in MCF7 cells for conducting CLIP-seq. These results elucidate unappreciated regulators of type I IFN signaling in breast cancer, offering potentially new targets to overcome cancer resistance to immune checkpoint blockade therapy.</p>
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Understanding regulation of mRNA by RNA binding proteins
… proteins' inherent affinities for different RNA sequences as well as other features such as translation and RNA structure which affect the accessibility of mRNA. The stabilities of mRNA transcripts are regulated by nonsense-mediated mRNA decay (NMD), a quality control degradation pathway. In this …
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Integrative analysis of transcriptomic data to elucidate regulators of pre-mRNA processing
… data across diverse high-throughput sequencing modalities, including RNA-seq, targeted 3' end sequencing, and CLIP-seq. Chapters 2 and 3 focus on the development and application of MAJIQ v2, a co-first author contribution that advances splicing analysis by enabling more accurate …
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Non-Coding RNA Features Critical to the Replication of HIV-1
<p>The HIV-1 genome contains RNA sequences and structures that control many aspects of viral replication including, but not limited to transcription, splicing, nuclear export, translation, packaging and reverse transcription. Despite this extensive existing catalogue of RNA sequences that are …
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Structure and Function of KH Domain in DDX43 and DDX53 Cancer Antigen Helicases
… to bind single-stranded (ss) RNA or ssDNA in a sequence specific manner. The helicase core domain contains two conserved RecA-like domains and unwinds double-strand nucleic acids in a sequence-unspecific manner; therefore, we hypothesize that the KH domain may play a critical role in …
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Dicer loss induces an oncogenic epigenetic switch in mesenchymal stem cells
… stem cell model. Upon exploring the consequences of Dicer deletion, we identify a specific let-7 regulated mid-embryonic program within the global de-repression of miRNA targets accompanying Dicer loss. We further observe within the landscape of let-7 regulated targets, an activation of …
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Discovery of miRNA-regulated processes in mammalian development
… "microRNAs" (miRNAs). These RNAs guide the sequence-specific inhibition of translation and destabilization of mRNA targets through short (6-7 nucleotide) stretches of complementarity between the 5' end of the miRNA (seed region) and the 3' untranslated region (UTR) of the targeted mRNA. …
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SR proteins in microRNA/mRNA biogenesis
… involved in such negative feedbacks, small RNA sequencing was used to identify differentially expressed miRNAs after SF2/ASF overexpression in an inducible stable cell line system. Among the 40 differentially expressed miRNAs, miR-7 is particularly interesting, because it is also predicted to …
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Functional study of miRNA-mRNA interactions in malaria mosquito An. gambiae
… of miRNAs in reproduction, I used small RNAs sequencing to monitor miRNAs expression and their association with Argonaute 1 (Ago1) and Argonaute 2 (Ago2) in the malaria mosquito Anopheles gambiae (An. gambiae) during the 72-h period immediately after blood feeding. I found the abundance and …