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Showing 1 to 9 of 9 for “"CIPA"”.
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Mechanism of active substrate delivery by the AAA+ protease adaptor CIpS
… The CIpS adaptor collaborates with the AAA+ CIpAP protease to recognize and degrade N-end rule substrates. CIpS binds the substrate's N-degron and assembles into a high-affinity CIpS-substrate-CIpA complex, but how the N-degron is transferred from CIpS to the axial pore of the AAA+ CIpA …
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Mechanistic studies of the ClpAP protease/
CIpA, a member of the Hspl00/Clp subset of the AAA+ superfamily, is an energy-dependent chaperone, disassembling and remodeling its protein substrates. CIpA also serves as the ATPase component of the ClpAP protease, where it is resonsible for binding specific substrates and unfolding and …
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Regulatory framework for adjudicators in the Malaysian construction industry
… Industry Payment and Adjudication Act 2012 (CIPA 2012) in 2014 is expected to improve the cash flow problems in the construction industry in Malaysia. Parallel with statutory needs to resolve disputes via adjudication, the statutory adjudicator exists to conduct the adjudication process. …
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The N-end rule degradation pathway : substrate recognition and staged delivery to the CIpAP protease by the CIpS adaptor protein
… delivers these substrates to the AAA+ protease CIpAP. Here, we present the first crystal structure of a bacterial N-end rule adaptor, CIpS, bound to a peptide mimic of an N-end rule substrate. This structure pioneered the understanding behind the basis of N-end rule recognition by CIpS. The CIpS …
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Engineering scFv Antibodies for Point-of-Care Diagnostics
… (scFv) antibodies with a cellulose binding tag (CipA) to tether it to paper, and a fluorescent protein (mCherry) to provide a detectable signal, the aim is to develop a paper-based sandwich immunoassay that can be used at bedside for faster results. Using phage-display screening, 14 scFv …
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Substrate selection by the ClpXP protease : a tail of destruction
(cont.) sites for CIpA and SspB; this spatial arrangement of signals allows for efficient modulation of proteolysis of ssrA-tagged proteins. Finally, additional substrates whose degradation may be regulated by the adaptor protein SspB were determined by identifying substrates captured in …
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Charakterisierung der Auswirkungen einer pathogenen Mutation im Neurotrophinrezeptorgen trkA im Zellkulturmodell
… and autonomous neuropathy type IV (HSAN IV or CIPA = congenital insensitivity to pain with anhidrosis). This autosomal recessive disorder manifests in loss of pain and temperature sensitivity, the inability to sweat and often also mental retardation. Due to the human pathogenic mutation R780P …
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SAXS and X-ray Crystallography Studies of the Cellulosome from Clostridium thermocellum
… and a trimodular C-terminal truncation of the CipA scaffoldin protein containing the ninth type-I cohesin module (CohI9), a linker, the X-module (X), and the type-II dockerin module (DocII). This structure revealed novel intertwining of scaffoldin molecules and extensive reciprocal contacts …
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Taming reactive phosphorus intermediates with organic and inorganic carriers
… from Me₂NPA, through isolated intermediates CIPA and [P₂A₂Cl][AlCl₄]. P₂A₂ was found to transfer P₂ efficiently to N-₃ CHD, 1,3-butadiene and (C₂H₄)Pt(PPh₃)₂ to form 1,2-cyclo-[P₂N₃]-, P₂(CHD)₂, P₂(BD)₂ and (P₂)[Pt(PPh₃)₂]₂ . A complete kinetic and computational study of the P₂(CHD)₂ …