Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"CHO-K1 cells"”.
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The Signaling Pathway of Oxysterol Induced Apoptosis in Chinese Hamster Ovary (CHO)-K1 Cells.
… It has been well established that vascular cells undergo apoptosis after uptake of oxidized low-density lipoprotein (oxLDL). Thus, an analysis of the signaling pathway of apoptotic induction by oxLDL is of value in understanding the development of atherosclerotic plaque. In order to …
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Pharmacological Studies of the Signal Transduction Pathways of the a 1a-Adrenbrgic Receptor Subtype
… that mediate the actions of the endogenous catecholamines, norepinephrine and epinephrine. Three 1a-AR subtypes (a1a, a1b, a1d) have been cloned and classified. cpa-ARs couple to phosphoinositide hydrolysis, adenylyl cyclase, and mitogen activated protein kinase (MAPR) pathways. In some systems, …
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Amino Acid Residues Implicated in the Interaction of Melanocortin Ligands and Their Receptors: A Study of MC2R Selectivity
… is now feasible. Using a general cell line like CHO-K1 cells, which do not express endogenous MCRs, we were able to create a heterologous expression system and test the selectivity of MC2R using analog variants of ACTH(1-24). Our results indicate an amino acid requirement in the C-terminal …
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Effect of continuous irradiation on proliferation of CHO-K1 and xrs-6 cells under low and high oxygen conditions
… continuous irradiation on Chinese Hamster Ovary cells, CHO-K1 (wild type) and xrs-6 cells (mutant), and 2) the effect of continuous irradiation under low (5%) and high (20%) ambient oxygen conditions. Other authors have found continuous irradiation at low dose rates to have beneficial effects on …
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Molecular and Pharmacological Studies of Alpha1-AR Subtypes and their Signaling Pathways
… two cell culture models were used: 1) DDTi-MF2 cells expressing aiB-AR’s were used to test the potency and maximum effects of aie-AR antisense oligomers and 2) transfected HEK293 cells expressing aio-AR’s were used to test a^-AR antisense oligomers. Separate oligomers containing mismatches …
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Functional analyses of Plasmodium Falciparum primary metabolic genes
… probes was developed and used to locate mitochondrial-, nuclear-, and apicoplast-targeting signal peptides in CHO-K1 cells. Taken together, this study has validated a plausible interactive network of primary metabolic plasmodial genes.
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Flow-Through Electroporation in Asymmetric Curving Microfluidic Channels
… macromolecules such as genes and drugs into cells. Genetic modification of the cell is critical for many cell and gene therapy techniques. Common electroporation protocols can only handle small volumes of cell samples. Also, most of the conventional electroporation methods require expensive …
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The mode of action of a lipid mobilising factor in cancer cachexia
… also shown to stimulate cyclic AMP production in CHO-K1 cells transfected with human -3 adrenergic receptors and inhibited by the -β3 antagonist SR59230A. LMF binding was also inhibited by SR59230A in isolated receptors. This suggests that LMF mediates its effects through a β3 adrenergic receptor. …
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Analyzing the Interactions Between Xenopus tropicalis MC2R, MC5R, and the MRAPs: Modeling the Regulation of Frog Interrenal Cells
… interrenal was verified through RT-PCR. Then CHO-K1 cells were transfected with plasmid constructs containing <em>X. tropicalis</em> MC2R, MC5R, MRAP2, and a mouse MRAP, and stimulated with ACTH or alpha-MSH. Levels of receptor activation were measured using a CRE-luciferase reporter gene. The …
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Applications of a novel assay to investigate variation in DNA repair in human populations.
… of the in vitro DNA repair assay. Cultured CHO K1 cells, used as a substrate, were damaged oxidatively under controlled conditions. A human lymphocyte extract was incubated with the cultured cells and the first stage of DNA repair-incision was carried out, producing increased strand breaks …
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Analysis, Optimization and Application of AAV Capsid Assembly using Escherichia coli
… and other therapies. Mammalian and insect cells have been utilized for rAAV production, but scaling to for the rapidly growing demand for rAAV in clinical trials remains challenging. Thus, a new AAV production technology is urgently needed. Meanwhile, AAV empty capsids as virus-like …
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Spatial proteomic investigation of monoclonal antibody expression in the Chinese hamster ovary (CHO) cell
… last three decades, the Chinese hamster ovary (CHO) cell has progressively established itself as a stable, high yielding mammalian expression system for the production of recombinant proteins – in particular monoclonal antibody (mAb) biotherapeutics. Despite the availability of other platforms, …
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Isoxazole Linked siRNA Cholesterol Conjugates by Catalyst Free Nitrile Oxide/Alkyne Cycloadditions
… presents the synthesis of isoxazole linked cholesterol conjugates by nitrile oxide/alkyne cycloadditions. Novel alkyne and oxime modified cholesterol derivatives were prepared and demonstrated to be efficient dipolarophiles and dipole precursors. Chapter 5 embraces the synthetic success …
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REGULATION OF GLYCOSYLATION IN MAMMALIAN CELL LINES: INSIGHTS FROM STRUCTURAL GLYCOMICS, ANALYSIS OF GLYCOSYLATION-RELATED GENES AND FUNCTIONAL RNAI SCREENS
… five commonly used mammalian cell lines, namely CHO-K1, BHK-21, HEK293, COS-7 and 3T3, were characterized by a combination of mass spectrometric and chromatographic techniques. Such glycomic profiling revealed several immunogenic non-human N-glycan structures in BHK-21 and 3T3 cells. The cell …
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Dual-Responsive Polymer and Hybrid Systems: Applications for Gene Delivery and Hydrogels
… utilizing dopamine as physically binding anchor group for the polymer chains. Here, a dopamine-based ATRP initiator was applied for the surface modification of the nanoparticles, which enabled a controlled polymerization technique via the “grafting-from” approach. Gene transfection …
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Polyelectrolyte Complexes and their Therapeutic Potential
… First, the delivery of genes into eukaryotic cells in vitro by means of new star-shaped polycations was explored. Second, the structure of ionic multicompartment micelles (MCMs) when complexed with polyions was studied and the performance of these nanostructures as delivery agents of …