Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"CHO-K1"”.
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The Signaling Pathway of Oxysterol Induced Apoptosis in Chinese Hamster Ovary (CHO)-K1 Cells.
… by oxLDL, we have used Chinese hamster ovary (CHO)-K1 cells treated with a potent oxysterol, 25-hydroxycholesterol (25-OHC). In the present study, we find that oxLDL can induce apoptosis in any cell types if cells present the specific receptors on their surface to take up oxLDL, and that …
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Effect of continuous irradiation on proliferation of CHO-K1 and xrs-6 cells under low and high oxygen conditions
… irradiation on Chinese Hamster Ovary cells, CHO-K1 (wild type) and xrs-6 cells (mutant), and 2) the effect of continuous irradiation under low (5%) and high (20%) ambient oxygen conditions. Other authors have found continuous irradiation at low dose rates to have beneficial effects on cells. …
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Pharmacological Studies of the Signal Transduction Pathways of the a 1a-Adrenbrgic Receptor Subtype
… that mediate the actions of the endogenous catecholamines, norepinephrine and epinephrine. Three 1a-AR subtypes (a1a, a1b, a1d) have been cloned and classified. cpa-ARs couple to phosphoinositide hydrolysis, adenylyl cyclase, and mitogen activated protein kinase (MAPR) pathways. In some systems, …
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Spatial proteomic investigation of monoclonal antibody expression in the Chinese hamster ovary (CHO) cell
… last three decades, the Chinese hamster ovary (CHO) cell has progressively established itself as a stable, high yielding mammalian expression system for the production of recombinant proteins – in particular monoclonal antibody (mAb) biotherapeutics. Despite the availability of other platforms, …
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Development and operation of a perfusion bioreactor for the cultivation of mammalian cells inside a sponge-like ceramic matrix
… und 5 mm Höhe konnte das Wachstum von CHO-K1, A549, MDCK und primären humanen Fibroblasten gezeigt werden, wobei Wachstum über die metabolische Reduktion von Resazurin und Masse elementaren Kohlenstoffs, Zellverteilung und -vitalität über Schnittfärbungen nachgewiesen wurden. Überdies …
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Amino Acid Residues Implicated in the Interaction of Melanocortin Ligands and Their Receptors: A Study of MC2R Selectivity
… is now feasible. Using a general cell line like CHO-K1 cells, which do not express endogenous MCRs, we were able to create a heterologous expression system and test the selectivity of MC2R using analog variants of ACTH(1-24). Our results indicate an amino acid requirement in the C-terminal …
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Molecular and Pharmacological Studies of Alpha1-AR Subtypes and their Signaling Pathways
… activated protein (MAP) kinase pathways. Using CHO-K1 cells, we investigated the coupling of mouse ccia-AR’s to ERK1/2 and JNK pathway activation. characterization of the expressed a t-AR in CHO-K1 cells by competition binding experiments with [3H]-prazosin, 5-methyl-urapidil and BMY-7378 (see …
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El papel de los lípidos en la estructura y función del receptor de acetilcolina nicotínico
… línea celular con metabolismo lipídico normal, CHO-K1 (Forsayeth et al., 1990c), establecimos el sistema de referencia. Posteriormente, generamos dos modelos experimentales con líneas celulares mutantes que eran deficientes en el metabolismo lipídico: una línea mutante de CHO-K1, denominada …
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REGULATION OF GLYCOSYLATION IN MAMMALIAN CELL LINES: INSIGHTS FROM STRUCTURAL GLYCOMICS, ANALYSIS OF GLYCOSYLATION-RELATED GENES AND FUNCTIONAL RNAI SCREENS
… five commonly used mammalian cell lines, namely CHO-K1, BHK-21, HEK293, COS-7 and 3T3, were characterized by a combination of mass spectrometric and chromatographic techniques. Such glycomic profiling revealed several immunogenic non-human N-glycan structures in BHK-21 and 3T3 cells. The cell …
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Caracterización de la interacción entre el anticonvulsivo Lamotrigina y el receptor de acetilcolina nicotínico
… con el AChR. Tal objetivo se asienta en el hecho que tanto el epifenómeno de la activación del AChR (luego de la unión con su ligando) es la permeación del Na+, al igual que el blanco farmacológico de la Lamotrigina (LTG), explotado en terapéutica. En una primera etapa, estudiamos mediante la …
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Functional analyses of Plasmodium Falciparum primary metabolic genes
… probes was developed and used to locate mitochondrial-, nuclear-, and apicoplast-targeting signal peptides in CHO-K1 cells. Taken together, this study has validated a plausible interactive network of primary metabolic plasmodial genes.
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Flow-Through Electroporation in Asymmetric Curving Microfluidic Channels
… EGFP, plasmid DNA into Chinese Hamster Ovary, CHO-K1, cells in our microfluidic chips. We show that the particles/cells undergo Dean flow in our asymmetric curving channels. We demonstrate that there are three main regimes for particle motion in our channels. At low flow rates (from 0 to …
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The mode of action of a lipid mobilising factor in cancer cachexia
… also shown to stimulate cyclic AMP production in CHO-K1 cells transfected with human -3 adrenergic receptors and inhibited by the -β3 antagonist SR59230A. LMF binding was also inhibited by SR59230A in isolated receptors. This suggests that LMF mediates its effects through a β3 adrenergic receptor. …
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Analyzing the Interactions Between Xenopus tropicalis MC2R, MC5R, and the MRAPs: Modeling the Regulation of Frog Interrenal Cells
… interrenal was verified through RT-PCR. Then CHO-K1 cells were transfected with plasmid constructs containing <em>X. tropicalis</em> MC2R, MC5R, MRAP2, and a mouse MRAP, and stimulated with ACTH or alpha-MSH. Levels of receptor activation were measured using a CRE-luciferase reporter gene. The …
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Efecto de las variaciones en los niveles de lípidos sobre el tráfico y propiedades del receptor de acetilcolina nicotínico en células CHO
… como modelo experimental a las células CHO-K1/A5, una línea celular desarrollada en nuestro laboratorio que expresa en forma heteróloga estable el AChR de tipo muscular adulto (α2βδε). En primer lugar, evaluamos los efectos de la depleción metabólica crónica del colesterol por la droga …
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Applications of a novel assay to investigate variation in DNA repair in human populations.
… of the in vitro DNA repair assay. Cultured CHO K1 cells, used as a substrate, were damaged oxidatively under controlled conditions. A human lymphocyte extract was incubated with the cultured cells and the first stage of DNA repair-incision was carried out, producing increased strand breaks …
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Analysis, Optimization and Application of AAV Capsid Assembly using Escherichia coli
… sequences (ITRs) mediated protein expression in CHO-K1 cells. The ARM_VP3 fusion protein was successfully obtained from E. coli and the in-vitro capsid assembly and DNA encapsidation resulted in VLPs and in protection of DNA from DNase I. However, these VLPs did not mediate protein expression in …
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