Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 101 for “"CHO cells"”.
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Protein expression and glycosylation in CHO cells
… production. Using Chinese Hamster Ovary (CHO) cells expressing recombinant human interferon-gamma (IFN-y) as the model system, this thesis investigated strategies that can potentially enhance heterologous protein expression or control the extent of protein glycosylation. There has been …
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Hyperosmolality effects on growth and morphology of CHO cells
… through which the hyperosmolality influences the CHO cells, we conceptualized a set-up where an industrial over-supplemented feed was used as an active osmotic agent, controlled for its specific effects by including a mannitol-supplemented feed to reach the same osmolalities and compare these two …
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Increasing IFN-[gamma] productivity in CHO cells through CDK inhibition
… are produced in Chinese Hamster Ovary (CHO) cells. Production in CHO cells, however, is often plagued by low productivity when compared with other host cell lines, including bacteria and yeast. For this reason, investigating ways of improving the productivity of CHO cells producing …
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MOLECULAR ROLES OF HSP27 IN IMPROVING MONOCLONAL ANTIBODY PRODUCTION OF CHO CELLS
CHO CELLS ARE THE MAMMALIAN CELL LINES OF CHOICE FOR THE PRODUCTION OF RECOMBINANT THERAPEUTIC BIOLOGICS INCLUDING THE RAPIDLY EXPANDING MONOCLONAL ANTIBODIES (MAB) AND BIOSIMILARS. THERE ARE OPPORTUNITIES TO EXPLORE THE USE OF ENGINEERED CHO CELL LINES WITH IMPROVED RECOMBINANT BIOLOGICS …
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Active hypothermic growth : a novel means for increasing total recombinant protein production by CHO cells
… glycoproteins produced by Chinese Hamster Ovary (CHO) cells are an important class of therapeutic molecules and investigating means of improving the production rate and product quality of these glycoproteins is therefore of great interest. Culturing CHO cells under mild hypothermia (30-33 ⁰C) …
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Human Estrogen Receptor(alpha) Activity on Estrogen Responsive, Integrated Chloramphenicol Acetyltransferase (Cat) Reporter Constructs in Chinese Hamster Ovary (Cho) Cells
The estrogen receptors endogenous to CHO cells did not induce transcription of chromosomal copies of the more complex 2ERETKCAT promoter. In transient transfections hER$\alpha$ regulated transcription of the three 2ERETKCAT cell lines. We found that an ER mutant containing only AF1 elicited a dose …
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Early selection of Chinese Hamster Ovary (CHO) cells using simultaneous label-free autofluorescence multi-harmonic (slam) microscopy and Fluorescence Lifetime Imaging Microscopy (FLIM) for biopharmaceutical cell line development
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-05-01
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Molecular Mechanism of Tetraspanin CD9 Mediated Cell Motility
… promoted cell motility in Chinese hamster ovary (CHO) cells. In contrast, inhibitors targeting protein kinase C or mitogen-activated protein kinase had no effect on CD9 driven CHO cell motility. Furthermore, inhibition of PI-3K activity in CHO cells by dominant/negative PI-3K cDNA transfection …
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Micro-bioreactor design for Chinese hamster ovary cells
… for the culture of Chinese Hamster Ovary (CHO) cells. There is an increasing demand for upstream development in high-throughput micro-bioreactors specifically for the recombinant CHO cell line, an important cell line for producing recombinant protein therapeutics. In order to translate a …
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Investigating the role of the UDP-galactose transporter SLC35A2 in the regulation of HIF signalling
… group as a novel regulator of HIF-α, and CHO cells that had lost SLC35A2 exhibited elevated HIF-α protein in normoxia. It is known that the absence of SLC35A2 in the Golgi leads to wide-ranging glycosylation defects. The underlying mechanisms linking SLC35A2 to HIF-α protein regulation …
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Binding of Mycobacterium tuberculosis to complement receptor type 3 expressed in mammalian cells : dependence on serum opsonins
… expressed in Chinese hamster ovary (CHO) cells, thereby circumventing the problems of competing receptors and endogenously synthesised complement, which are inherent in studies with mononuclear phagocytes. The surface expression and functional activity of CR3 were confirmed by …
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Genetic control of death in Chinese Hamster Ovary cultures
… systems, including Chinese Hamster Ovary (CHO) cultures, is the inability to maintain viability of these cultures over extended periods of time. This inability translates into lower final protein titers and higher protein production and recovery costs. This thesis was undertaken to better …
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The Engineering of Chinese Hamster Ovary Cells to Achieve More Efficient Gene Amplification for Improving Biopharmaceutical Development
… times of obtaining Chinese hamster ovary (CHO) cells capable of producing high quantities of therapeutic proteins. It addresses the specific time bottlenecks associated with developing high producing CHO cell lines and reviews various methods that are employed to alleviate these …
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Redox Regulation of Cell Fate
… redox status of Chinese hamster ovary (CHO) cells treated with electrical potentials indicate that electrical potentials may be a useful technology for controlling the intracellular redox environment. A rationally-designed FRET biosensor expressed in CHO cells was used for reporting …
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Analyzing the Interaction Between Melanocortin 5 Receptor, Melanocortin Receptor Accessory Protein 1, and Melanocortin 2 Receptor in Rainbow Trout
<p>Using CHO cells we sought to explore and characterize the functional relationship of rainbow trout MC5 receptor (rtMC5R) with zebrafish MRAP1 (zfMRAP1) and rainbow trout MC2 receptor (rtMC2R), as well as how the effect of such relationships may play a significant role in …
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Identification of the receptor for cellular internalization of an anti-nucleic acid antibody, 3D8 scFv
… antibodies (Ab) can internalize in a variety of cells. An anti-nucleic acid antibody, murine 3D8 single chain variable fragment (scFv) Ab, has been known to be an internalizing antibody that enters the cells by caveolae/lipid-raft-mediated endocytosis. However, cell surface receptor for 3D8 scFv …
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New experimental approaches to the population balance equation:Eulerian and Lagrangian viewpoints
… variability in an asynchronous population of cells can be generally related to genetic differences, to different positions in the cell cycle, to the exposure of a heterogeneous environment, or to stochastic variations due to the low number of molecules in individual cells. To experimentally …
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Dielectrophoresis study of electroporation effects on dielectric properties of biological cells
… affects the dielectric properties of cells. Dielectric measurement techniques can provide a label-free and non-invasive modality to study this phenomenon. In this thesis we introduce a dielectrophoresis (DEP) based technique to study changes in the cytoplasm conductivity of single …
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Toxic effects on model mammalian cells by monohalogenated disinfection byproducts: haloacetic acids and haloacetonitriles
… cycle alteration using Chinese hamster ovary (CHO) cells as a model mammalian cell system. Both HAAs and HANs are alkylating agents with a halogen atom attached on their carbon atom next to the functional group. The potency of cytotoxicity and genotoxicity induction by monoHAAs and monoHANs is …
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The synthesis and characterization of new sulfoxide complexes of ruthenium and their potential as anti-cancer agents
… are tested in vitro using Chinese hamster ovary (CHO) cells. The biological data indicate that the complexes accumulate in CHO cells, without hypoxic selectivity. No toxicity is observed at the complex concentrations used (1.0 mM for 1 - 4, 7; 0.50 mM for 5 and 6) despite evidence that all seven …
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