Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 654 for “"CHO"”.
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A short & sweet story of CHO
<p>The solid state nuclear magnetic resonance (SSNMR) study of nearly intact plant cell walls along with the computational study of glucose and malonic acid are presented. Previously unassigned <sup>13</sup>C chemical shifts are presented for the various carbohydrates in the primary plant cell wall …
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Protein expression and glycosylation in CHO cells
… production. Using Chinese Hamster Ovary (CHO) cells expressing recombinant human interferon-gamma (IFN-y) as the model system, this thesis investigated strategies that can potentially enhance heterologous protein expression or control the extent of protein glycosylation. There has been …
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Hyperosmolality effects on growth and morphology of CHO cells
… through which the hyperosmolality influences the CHO cells, we conceptualized a set-up where an industrial over-supplemented feed was used as an active osmotic agent, controlled for its specific effects by including a mannitol-supplemented feed to reach the same osmolalities and compare these two …
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Gentechnische Verfahren zur Erzeugung und Selektion von hochproduzierenden CHO-Zellen
… als Selektionsmarker in CHO-DG44-Zellen für die Anreicherung von Hochproduzenten geeignet ist. Eine weitere Möglichkeit, die Expressionsrate eines stabil integrierten Produktgens zu erhöhen, ist der Einsatz von cis- und transwirkenden genetischen Elementen. In der …
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Increasing IFN-[gamma] productivity in CHO cells through CDK inhibition
… are produced in Chinese Hamster Ovary (CHO) cells. Production in CHO cells, however, is often plagued by low productivity when compared with other host cell lines, including bacteria and yeast. For this reason, investigating ways of improving the productivity of CHO cells producing …
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A computational framework for predicting CHO cell culture performance in bioreactors
Breaking the trade-off between speed and productivity is a key milestone across industries. In particular, in the biopharmaceutical industry this trade-off is exacerbated by a highly regulated environment, which hinders continuous improvement and fixes future manufacturing costs. Given the …
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Developing a robust harvest for high cell density CHO cell culture
Thesis: S.M., Massachusetts Institute of Technology, Department of Biological Engineering, May, 2020
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MOLECULAR ROLES OF HSP27 IN IMPROVING MONOCLONAL ANTIBODY PRODUCTION OF CHO CELLS
CHO CELLS ARE THE MAMMALIAN CELL LINES OF CHOICE FOR THE PRODUCTION OF RECOMBINANT THERAPEUTIC BIOLOGICS INCLUDING THE RAPIDLY EXPANDING MONOCLONAL ANTIBODIES (MAB) AND BIOSIMILARS. THERE ARE OPPORTUNITIES TO EXPLORE THE USE OF ENGINEERED CHO CELL LINES WITH IMPROVED RECOMBINANT BIOLOGICS …
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Optimisation of CHO Cell Line Development Using Hybrid Modelling for Antibody Therapeutics
… and autoimmune disorders. Chinese Hamster Ovary (CHO) cells are the primary host for mAb production due to their flexible karyotype and human-like post-translational modifications. However, their flexibility poses challenges due to their genetic instability and expression variability upon longer …
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Untersuchungen zum enzymatischen und immunchemischen Nachweis der Phytanoyl-CoA-Hydroxylase in CHO-Zellen
… kultivierter Zellen, insbesondere von CHO-Zellen, erlaubt. Dieses war deswegen gefordert, weil der Test zur Charakterisierung induzierter CHO-Zell-Mutanten eingesetzt werden sollte, die die Merkmale eines PTS 2-Import-Defektes aufweisen. Dieser Nachweis sollte durch eine Messung der …
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The Signaling Pathway of Oxysterol Induced Apoptosis in Chinese Hamster Ovary (CHO)-K1 Cells.
… by oxLDL, we have used Chinese hamster ovary (CHO)-K1 cells treated with a potent oxysterol, 25-hydroxycholesterol (25-OHC). In the present study, we find that oxLDL can induce apoptosis in any cell types if cells present the specific receptors on their surface to take up oxLDL, and that …
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Spatial proteomic investigation of monoclonal antibody expression in the Chinese hamster ovary (CHO) cell
… last three decades, the Chinese hamster ovary (CHO) cell has progressively established itself as a stable, high yielding mammalian expression system for the production of recombinant proteins – in particular monoclonal antibody (mAb) biotherapeutics. Despite the availability of other platforms, …
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Data-driven Dynamic Control Scheme for Antibody Producing CHO Cell Cultures in Fed-batch
… in the context of antibody producing CHO cell cultures in fed-batch bioreactors. Multivariate monitoring techniques in biopharmaceuticals has resulted in the generation of large amounts of data comprising real-time measurements of critical quality and performance attributes, and if …
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The post-translational methylation of arginine in the glycine arginine rich region of CHO nucleoin
… to the nucleoplasm and not to the nucleoli of CHO cells. This version of nucleolin was subsequently used to probe the in vivo interactions of nucleolin with other non-ribosomal nucleolar proteins. In support of previous work, DGAR caused endogenous B23 to redistribute to the nucleoplasm, …
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Active hypothermic growth : a novel means for increasing total recombinant protein production by CHO cells
… glycoproteins produced by Chinese Hamster Ovary (CHO) cells are an important class of therapeutic molecules and investigating means of improving the production rate and product quality of these glycoproteins is therefore of great interest. Culturing CHO cells under mild hypothermia (30-33 ⁰C) …
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Rationale Entwicklung rekombinanter CHO-Zellen basierend auf Histonanalyse und RNA-Seq: Identifizierung und Verwendung stabiler Integrationsorte
Chinese hamster ovary (CHO) cells represent the most prevalent platform for biopharmaceutical protein production. Random integration of a transgene into the genome during cell line development often leads to a loss of productivity during upstream processing. This is due to loss of gene copies or …
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Development and application of a microfluidic platform for single-cell cultivation of CHO suspension cell lines
… enzymes. Predominantly, Chinese hamster ovary (CHO) cells are utilized in these bioproduction processes, as they can easily be genetically modified and thereby are able to express biopharmaceuticals that are human-compatible, are well-established in industrial-scale cultivation, and achieve high …
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