Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"CHK2"”.
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Novel Role of CHK2 in the Intrinsic and Extrinsic Apoptosis Pathway
… malignant transformation. Checkpoint kinase 2 (CHK2) is an evolutionarily conserved serine/threonine protein kinase that is activated in response to DNA double strand breaks. Upon activation by the DNA damage transducer kinase, ATM, CHK2 phosphorylates a wide variety of down stream effectors to …
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Role of Chk1 and Chk2 in mitotic checkpoint control in vertebrate cells
Two conserved protein kinases, Chk1 and Chk2, are activated in response to genotoxic stress and mediate multiple cell cycle checkpoint mechanisms that ensure genomic integrity. The establishment of mitotic checkpoint delay in response to DNA damage or incompletely replicated DNA is conventionally …
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The Role of Chk2 and Wee1 Protein Kinases during the Early Embryonic Development of Xenopus laevis
… understand the roles of two protein kinases, Chk2/Cds1 and Wee1, during checkpoint signaling in the developing embryo. The DNA damage checkpoint is mediated by the Chk2/Cds1 kinase. Conflicting evidence implicates Chk2 as an inhibitor or promoter of apoptosis. To better understand the …
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The functional and molecular characterisation of the novel DNA damage repair protein Bod1L
… evidence that Bod1L acts as a regulator of Chk2 activation, acting to release Chk2 from the well-characterised inhibitory Chk2/PP2A-B56 phosphatase complex upon DNA damage, thus allowing the kinase to phosphorylate downstream substrates. <br/><br/>Overall, Bod1L makes three critical …
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The effect of UVA irradiation on the DNA damage response in a human keratinocyte cell line
… 4h following irradiation with 100 kJ m-2 UVA. Chk2 phosphorylation peaked immediately following UVA irradiation. Inhibition of ATM prior to UVA exposure resulted in a temporal decoupling of H2AX and Chk2 phosphorylation with peak H2AX activation occurring at a later timepoint whilst p-Chk2 …
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Circumventing Cisplatin Resistance In Ovarian Cancers Through Reactivation of P53 By Non-Cross-Resistant Platinum Analogs
… resistant cell lines expressed low levels of Chk2, a kinase responsible to phosphorylate p53 at Ser20, as a common feature. It was confirmed, through the generation of Chk2 knock-out clones from A2780 cells using the CRISPR/Cas9 system, that Chk2 is essential for cis-Pt to mediate …
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Biochemical and Molecular Characterisation of the FADDosome
… II, our group discovered that the ATR, Chk1 and Chk2 kinases are activated in 5-FU-treated HCT116 cells and play a key role in the 5-FU-induced DNA damage signal transduction towards caspase-10 upregulation and FADDosome formation. It was found that activation of Chk1 and Chk2 as well as …
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Effect of TIS21 on DNA Damage Repair Induced by Etoposide
… was performed. The phosphorylation level of Chk2T68 and p53S20 in the present of etoposide in TIS21 infected Huh7 cells (harboring mutant p53) was significantly decreased as compared to LacZ infected cells. In addition to wild type p53 harboring cells such as MCF7, HeLa, and H9C2 cell lines …
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Tyrosine 370 Phosphorylation of Atm Positively Regulates Dna Damage Response
… blocks EGFR and ATM association, hinders CHK2 activation and subsequent foci formation, and increases radio-sensitivity. Thus, we reveal a critical mechanism by which EGFR directly regulates ATM activation in DNA damage response and ATM Y370 phosphorylation may serve as a biomarker for …
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Roles for C-ABL and P53 in bone homeostasis and DNA damage response
… proteins such as TopBP1, Brca1, activation of Chk2 and Chk1, phosphorylation of p53 at Ser15 and induction of p53 target genes, cell cycle checkpoints and DNA repair of ssDNA breaks. These findings were confirmed by c-Abl reconstitution. Further studies indicate that c-Abl functions upstream of …
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Interlocking mechanisms regulating the circadian clock response to DNA damage
… Mutated), ATR (ATM- and Rad3-related) and CHK2 (Checkpoint Kinase 2) regulate radiation-induced phase shifts. Lastly, we found that CLOCK (Circadian Locomotor Output Cycles Kaput) and CRY1 (CRYPTOCHROME 1) were phosphorylated in response to radiation. Taken together, these results indicate …
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Investigation of The Roles of Asf1 and Caf-1-Mediated Chromatin Assembly In The Human Dna Damage Response
… and CAF-1 render the rapid inactivation of ATM Chk2 pathway via facilitating histone acetylation to allow the recruitment of ATM to DSBs for efficient repair by non homologous end joining. Furthermore, in response to DSB damage, ASF1A is specifically phosphorylated by DNA-PKcs and potentially …
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ATM-Dependent ERK Signaling in Response to DNA Double Strand Breaks
… BrdU photolysis increased phosphorylation of Chk2 (at thr-68) and p53 (at ser-15). p53 phosphorylation was reduced by the ATM inhibitor caffeine, and H2AX phosphorylation was greatly reduced in AT cells, confirming that phosphorylation was primarily ATM-dependent. We also examined the effects …
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Integration of the phosphorylation-dependent signaling in the DNA damage response network : implications for cancer
… family that functions in parallel with CHK1 and CHK2 to integrate DNA damage signaling responses and cell cycle arrest in mammalian cells.
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PIKK-dependent activation of the DNA damage response by long wavelength ultraviolet radiation
… of UVA-induced γH2AX following. UVA-induced CHK2 and p53 activation followed similar patterns in both ATM inhibited and uninhibited samples, and suggested that both components are linked to an ATM independent pathway. UVA-induced PNKP activation was delayed with the addition of an ATM …
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Identification and Characterization of the DNA Damage Response Function of Human Rif1 and a Search For Human Rap1 Interacting Factors
… was not affected by reduced function of BRCA1, Chk2, Nbsl, and Mrell. RNAimediated Rifl inhibition resulted in increased radiosensitivity, indicating that Rifl contributes to ATM-mediated protection against exposure to ionizing radiation. In order to more fully understand telomere length …
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Cell Cycle Regulation by Post-Translational and Post-Transcriptional Mechanisms in an Anaerobic Extremist- the Anoxia Tolerant Turtle, Trachemys Scripta Elegans
… by signaling cascades (Akt/GSK-3β and ATR/Chk2) that are known to regulate G1/G0 phases of the cell cycle. Importantly, there is extensive regulation of Cyclin D1 protein by (1) Akt/GSK-3β signaling, (2) post-translational modification, (3) an AU-rich region, and (4) microRNA-induced …
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TARGETING THE DNA DAMAGE RESPONSE PATHWAY FOR THE TREATMENT OF AGGRESSIVE THYROID CANCERS, IN VITRO STUDIES
… of the DDR kinase proteins ATR, Chk1 and ATM, Chk2 as opposed to the p53-proficient and LENV- sensitive cell lines (TPC-1, IHH-4). Notably, DX treatment, generally inducing ATM/Chk2 kinases activation, caused in p53- defective TC cells also the peculiar activation of ATR/Chk1 kinases as an …
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Modulation of Clr-b Ligand for the Inhibitory NKR-P1B NK Cell Receptor in Response to Transformation and Genotoxic Stress
… response effectors including ATM, ATR, Chk1, Chk2 and p53. Furthermore, the loss of transcript can by uncoupled from the loss of surface protein following administration of proteasomal inhibitors. Lastly, inhibition of the autocatalytic hammerhead ribozyme harboured within the 3’UTR of the …
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Replicative Senescence is ATM-Driven, Reversible, and Delayed by Reduced ATM Activity at Low Oxygen
… tested. Using specific inhibitors of ATM, ATR, Chk2, and Chk1, we show that replicative senescence is solely due to ATM signaling, and that deficiency in TRF2 is the primary reason for ATM activation at critically short telomeres. In further support of this, we find that ataxia-telangiectasia …
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