Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"CENP-A"”.
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Centromere Identity and the Nature of the Cenp-A-Containing Nucleosome
… The histone H3 variant centromere protein A (CENP-A) replaces H3 in nucleosomes at the centromere and is the best candidate to provide this epigenetic mark. This thesis aims to understand the features of the CENP-A nucleosome that impart its ability to mark and stabilize functional …
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Structural and Mechanistic insights into Centromere Specification and Kinetochore Regulation
… chromosome segregation: 1) centromere protein A (CENP-A), the histone H3 variant which epigenetically specifies the centromere which forms the platform onto which the kinetochore assembles and 2) the chromosomal passenger complex (CPC) which regulates kinetochore-microtubule interactions to ensure …
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Investigating the Role of Inner Kinetochore Higher-Order Assembly in Kinetochore Function
… that is designated by the histone H3 variant CENP-A. CENP-A nucleates kinetochore assembly at centromeres but also easily mislocalizes to non-centromeric DNA. Interestingly, non-centromeric CENP-A typically does not recruit kinetochores. Kinetochores only assemble at loci where there is a very …
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Mechanisms for the propagation and recognition of human centromeres
… mark that specifies the centromere, the CENP-A nucleosome. I defined the molecular basis for this control, as well as an additional level of control downstream of the cyclin-dependent kinases. By identifying and dissecting the molecular features of this two-step regulatory paradigm, I …
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Centromere identification and inactivation on a neo-Y chromosome fusion in threespine stickleback fish (Gasterosteus aculeatus)
… histone, also called centromere protein A (CENP-A), or a genetic mechanism involving deletion or mutation of centromeric DNA. However, little is known about this process in naturally occurring dicentric chromosomes. For my dissertation, I characterized the mechanism of centromere …
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Determining Molecular Mechanisms of Cell Division in Fission Yeast by Testing Major Assumptions of the Search, Capture, Pull, and Release Model of Contractile-Ring Assembly
… A popular standard is the histone H3 variant CENP-A, Cse4 in budding yeast. Anaphase centromere clusters contained about 4-fold more Cse4 in <i>S. cerevisiae</i> and about 40-fold more CENP-A (Cnp1) in <i>S. pombe</i> than assumed. These results suggest CENP-A molecules exceed the number of …
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Functional consequences of rapid evolution at Drosophila centromeres
… function of the centromeric histone variant CENP-A/Cid. Furthermore, I found rapid evolution had not only affected the primary sequence of kinetochore proteins in Drosophila, but also the composition of the kinetochore itself, since the young gene Umbrea gained essential centromere function …
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Lokalisationsmikroskopie mit mehreren Farben und ihre Anwendung in biologischen Präparaten
… structures like centromere protein clusters (CENP- A, CENP-B and CENP-C) within the human kinetochore. In addition, the axial position of the localization data within the structure can be determined with a precision of about 50nm. A model for the distribution of the fluorophores in tobacco …
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Funktionelle Charakterisierung und Positionierung von Drosophila Cenp-C im Zentromer/Kinetochor-Komplex
… des gesamten Zellzyklus am CKC lokalisieren. Cenp-A, eine für das Zentromer spezifische Variante des Histon H3, war bis vor Kurzem die einzige bekannte ubiquitäre konstitutive Komponente des CKC. Mit der Identifizierung des stark abgeleiteten Cenp-C von Drosophila wurde eine weitere …
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Mitotic slippage by ATP depletion and its mechanism
… co-treatment with 2-DG and NaN3. Decrease of p-CENP-A and p-histone H3 (T3) on kinetochore during the mitotic slippage also supported that kinase activity of Aurora B is inhibited by co-treatment with 2-DG and NaN3. Moreover, phosphorylation of Histone H3 at Thr 3 by Haspin was especially …