Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"CDR2"”.
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Characterization of the Paraneoplastic Cerebellar Degeneration Antigen Cdr2
… using PCD antisera. We have demonstrated that cdr2, which encodes a cytoplasmic coiled-coil leucine zipper protein, is the only cdr gene expressed in PCD-associated tumors, and is therefore the true PCD tumor antigen. We have isolated the mouse cdr2 homologue and examined expression of the mRNA …
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From Naturally Occurring Tumor Immunity to Supernatural T Cells: Isolation and Characterization of a Murine T Cell Receptor Specific for Human Breast and Ovarian Tumor Antigen Cdr2
… natural immune response against a protein called cdr2 in their breast and ovarian carcinomas, and thus provide an innovative starting point for understanding how to harness the immune system to fight cancer. We previously demonstrated cdr2-specific cytotoxic T lymphocytes (CTL) in the peripheral …
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Structure, Function and Engineering of Peptide -Mhc Binding Receptors
… In contrast, other CDRs (CDR1, and especially CDR2) of the TCR are typically located over the MHC helices and thus may not be positioned to interact directly with peptide. Mutations in these CDRs might be expected to yield high-affinity but reduced peptide specificity (i.e. interactions with …
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Transcriptional Regulation of Azole Antifungal Resistance in Candida albicans
… cassette (ABC) transporter genes CDR1 and CDR2 have been shown to be regulated by a zinc cluster transcription factor Tac1p. A gain-of-function mutation in the gene encoding this transcription factor is responsible for the overexpression of CDR1 and CDR2. To identify the Tac1p regulon, we …
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Engineering the T Cell Receptor to Protect Host Cells in Superantigen-Induced Septic Shock
… revealing a binding hotspot involving Vbeta TCR CDR2. Additionally, the Valpha CDR2 region of the TCR was shown to be critical for the stabilization of the proposed TCR:SAg:pMHC ternary complex. Guided by these results, TCR mutants were engineered by directed mutation and yeast surface display to …
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Cellular and Molecular Biological Investigations Into the Interaction Between Candida Albicans and the Vaginal Epithelium
… by microarray analysis and included CDR1 and CDR2. Analysis of estrogen-exposed strain DSY654, that lacks CDR1 and CDR2 coding sequences, showed a significantly decreased number of germ tube-forming cells in the presence of estrogen. PDR16 was the most highly up-regulated gene in strain DSY654 …
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Expression Pattern of Drug-Resistance Genes in Matched Clinical Isolates of Candida albicans at Variable Fluconazole Exposure Times
… overexpression of a) the encoding genes CDR1 and CDR2 for the ABC Transporters, b) the Major Facilitator Transporter encoding gene MDR1, or c) ERG11, the azole target gene. Previous studies have shown gene expression in a matched set of clinical isolates is maximized at the drug concentration that …
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Characterization of anti-DNA antibody with Tat peptide sequence in CDR
… Tat 펩티드 (GRKKRRQRRRPPQ)를 3D8 scFv의 VH-CDR1, VH-CDR2 및 VH-CDR3부위에 교체, 삽입된 scFv 변이체를 클로닝한 후, 단백질을 박테리아세포에서 발현, 정제하였다. 세 종류의 변이체 중에서 수용성 단백질로서의 발현률이 가장 높은 VH-CDR3 부위가 Tat으로 교체된3D8 scFv 변이체 (3D8s-HC3/Tat)의 DNA 결합력, DNA 가수분해능력, 세포내 침투능, 및 핵내로의 이동여부를 조사하였다. ELISA 및 SPR (surface plasmom resonance) …
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Structural studies of the human polymeric immunoglobulin receptor
… IgA binding, contains a single helical turn. CDR2, the main determinant in binding to pIgM is very short and contains a potentially critical glutamic acid involved in pIgM binding. CDR3 points away from the other CDRs, preventing dimerization of D1 analogous to the variable heavy and light …
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ERG11-Mediated Azole Resistance in Candida albicans
… the efflux transporter genes <em>CDR1</em>, <em>CDR2</em>, and <em>MDR1</em> is a common mechanism of drug resistance in <em>C. albicans</em> and the majority of previous investigations pertained to defining mechanisms of transcriptional regulation of efflux transporters. Alternatively to efflux …
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Klonierung und Charakterisierung eines polymorphen MHC Kl. I-Moleküls der LEW.1F-Ratte, das präferentiell Va8.2-exprimierende CD8-T-Zellen expandiert
… ist die Komplementarität-bestimmende Region 2 (CDR2) der alpha-Kette. Die charakteristischen Sequenzen von CDR1alpha und CDR3alpha dagegen sind für die präferentielle Expansion von AV8S2-positiven CD8-T-Zellen durch Af essentiell.
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Investigation of Clinically Relevant Fluconazole Resistance Mechanisms in the Fungal Pathogen Candida parapsilosis
… shown to elevate the expression of MDR1 and CDR1/CDR2 respectively and directly contribute to fluconazole resistance in Candida albicans. Our lab previously identified three resistant isolates with upregulated CpMDR1 expression that contained CpMRR1 mutations, while CpTAC1 mutations were found in …
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Engineering and characterizing human T cell receptors for cancer immunotherapies
… complementarity-determining regions (CDR1, CDR2, and CDR3), which interact with the peptide (pep)MHC antigen. The conserved docking angle of the six CDRs over the pepMHC antigens is thought to confer maximal signaling capabilities and it places the two most hypervariable loops (CDR3s) over …
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Engineering and characterization of human single-chain T cell receptors
… encoded regions of the TCR (i.e. CDR1 and CDR2) are positioned primarily over the MHC helices whereas the diverse regions derived from the junctions of somatically rearranged gene segments (i.e. CDR3) are positioned primarily over the peptide. Over the last 15 years, the Kranz lab has …
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Identification of rat NKT cells and molecular analysis of their surface receptor mediated activation
… daß Keimbahn kodierte der b-Kettenbereiche (CDR2 oder CDR4) speziesspezifische Bereiche des CD1d erkennen. Weiterhin wurde gefunden, das die Zytokinsekretion der Zellinien durch CD80 spezifische monoklonale Antikörper inhibiert wurde, was eine wichtige Rolle der CD80-CD28 Interaktion bei der …
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Engineering soluble, high affinity T cell receptor domains for detection of staphylococcal and streptococcal exotoxins
… within the complementarity determining region 2 (CDR2) loop of the Vβ. The engineered, high-affinity Vβ22 protein was specific for SEA, in that it did not bind to two other closely related enterotoxins SEE or SED, providing information on the SEA residues possibly involved in the interaction. …
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An Investigation into Clinically Relevant Determinants of Azole Resistance in Candida albicans
… of the efflux pump encoding genes CDR1, CDR2, and MDR1 as well as increased expression of ERG11, encoding the azole target (14α-lanosterol demethylase, also known as CYP51) are all primary mechanisms of azole resistance that arise in azole-resistant clinical isolates. These changes are …
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Novel Determinants That Influence Azole Susceptibility in Candida glabrata and Candida albicans
… the ABC transporter genes <em>CDR1 </em>and <em>CDR2 </em>in azole-resistant clinical isolates of <em>C. albicans </em>that carry activating mutations in the transcription factor Tac1p. We show here that deleting <em>RTA3 </em>in an azole-resistant clinical isolate carrying a Tac1p activating …