Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"CDR1"”.
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Novel Determinants That Influence Azole Susceptibility in Candida glabrata and Candida albicans
… encoding the multidrug resistance transporters Cdr1, Pdh1, and Snq2. However, the specific relative contribution of these transporters to resistance is not known. In order to determine this, the <em>SAT</em><em>1 </em>flipper method was used to delete <em>CDR1</em>, <em>PDH1</em>, and <em>SNQ2 …
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Structure, Function and Engineering of Peptide -Mhc Binding Receptors
… TCR variants. In contrast, other CDRs (CDR1, and especially CDR2) of the TCR are typically located over the MHC helices and thus may not be positioned to interact directly with peptide. Mutations in these CDRs might be expected to yield high-affinity but reduced peptide specificity (i.e. …
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Effect of disruption of 3D8 complementarity-determining regions on properties of 3D8 antibody
… affinity to DNA than VH wild type. Therefore, L-CDR1 in 3D8 scFv format is likely to be responsible for DNA-binding activity. Sc-H3i and sc-L3i still retained the heparin-binding activity similar to 3D8 scFv. Moreover, sc-H3i, sc-L3i and H-3i entered the HeLa cells and localized in cytosol of the …
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Transcriptional Regulation of Azole Antifungal Resistance in Candida albicans
… The ATP binding cassette (ABC) transporter genes CDR1 and CDR2 have been shown to be regulated by a zinc cluster transcription factor Tac1p. A gain-of-function mutation in the gene encoding this transcription factor is responsible for the overexpression of CDR1 and CDR2. To identify the Tac1p …
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Nickel-Koordinationsstellen in einem T-Zellrezeptor/Peptid/MHC-Komplex
… sowie in der TCR a-Kette zwei Tyrosine (Tyr30 in CDR1 und Tyr94 in CDR3) als essentiell für die Ni-Reaktivität identifiziert werden. Im Fall von His81 wurde lediglich ein Austausch zu Tyr toleriert, während für beide Tyrosine sowohl Ala wie Phe Mutationen einen kompletten Reaktivitätsverlust …
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Engineering Soluble, High Affinity Receptor Antagonists for Bacterial Exotoxins
… the Vbeta was performed through extension of the CDR1 loop. Soluble forms of the high-affinity Vbeta regions were tested for their ability to inhibit SEB-mediated T cell cytotoxicity in vitro. As the affinity of the Vbeta regions increased, the amount of protein needed to neutralize 50% of the …
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An Investigation into Clinically Relevant Determinants of Azole Resistance in Candida albicans
… expression of the efflux pump encoding genes CDR1, CDR2, and MDR1 as well as increased expression of ERG11, encoding the azole target (14α-lanosterol demethylase, also known as CYP51) are all primary mechanisms of azole resistance that arise in azole-resistant clinical isolates. These changes …
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Cellular and Molecular Biological Investigations Into the Interaction Between Candida Albicans and the Vaginal Epithelium
… were defined by microarray analysis and included CDR1 and CDR2. Analysis of estrogen-exposed strain DSY654, that lacks CDR1 and CDR2 coding sequences, showed a significantly decreased number of germ tube-forming cells in the presence of estrogen. PDR16 was the most highly up-regulated gene in …
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Transcriptional Regulation of Azole Antifungal Resistance and Tolerance in Candida Glabrata
… expression of multidrug transporter genes <em>CgCDR1</em> and <em>CgPDH1</em>. It was previously reported that azole-resistant mutants (MIC ≥ 64 μg/ml) of strain 66032 (MIC = 16 μg/ml) similarly show coordinate <em>CDR1</em>-<em>PDH1</em> upregulation, and in one of these (F15) a putative …
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Expression Pattern of Drug-Resistance Genes in Matched Clinical Isolates of Candida albicans at Variable Fluconazole Exposure Times
… due to overexpression of a) the encoding genes CDR1 and CDR2 for the ABC Transporters, b) the Major Facilitator Transporter encoding gene MDR1, or c) ERG11, the azole target gene. Previous studies have shown gene expression in a matched set of clinical isolates is maximized at the drug …
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ERG11-Mediated Azole Resistance in Candida albicans
… of the efflux transporter genes <em>CDR1</em>, <em>CDR2</em>, and <em>MDR1</em> is a common mechanism of drug resistance in <em>C. albicans</em> and the majority of previous investigations pertained to defining mechanisms of transcriptional regulation of efflux transporters. …
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Characterization of anti-DNA antibody with Tat peptide sequence in CDR
… 아미노산으로 구성된 Tat 펩티드 (GRKKRRQRRRPPQ)를 3D8 scFv의 VH-CDR1, VH-CDR2 및 VH-CDR3부위에 교체, 삽입된 scFv 변이체를 클로닝한 후, 단백질을 박테리아세포에서 발현, 정제하였다. 세 종류의 변이체 중에서 수용성 단백질로서의 발현률이 가장 높은 VH-CDR3 부위가 Tat으로 교체된3D8 scFv 변이체 (3D8s-HC3/Tat)의 DNA 결합력, DNA 가수분해능력, 세포내 침투능, 및 핵내로의 이동여부를 조사하였다. ELISA 및 SPR (surface plasmom …
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Structural studies of the human polymeric immunoglobulin receptor
… the complementarity determining regions (CDRs). CDR1, the primary determinant in dimeric IgA binding, contains a single helical turn. CDR2, the main determinant in binding to pIgM is very short and contains a potentially critical glutamic acid involved in pIgM binding. CDR3 points away from the …
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Molecular Analysis of Phenotypic Diversity in Human Fungal Pathogens
… but in part mediated by the ABC transporter CDR1. Further, I showed that C. auris is capable of filamentous growth in response to Hsp90 depletion or inhibition. Transcriptomic analysis upon HSP90 depletion showed that filamentous growth is associated with upregulation of putative cell wall …
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Challenging the Paradigm of Clinical Triazole Resistance in Aspergillus fumigatus
… albicans triazole efflux pump-encoding gene, CDR1. However, deletion of either abcA or atrI in triazole-resistant clinical isolates which overexpress these genes, did not result in a significant change in triazole susceptibility. Finally, upon demonstrating that the canonical mechanisms of …
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Design and Characterization of RFIC Voltage Controlled Oscillators in Silicon Germanium HBT and Submicron MOS Technologies
… were fabricated using the Motorola 0.4 μm CDR1 SiGe BiCMOS process, which consists of four interconnected metal layers and a thick copper (10 μm) metal bump layer for improved inductive components. The VCO implementations are targeted to meet the stringent phase noise specifications for the …
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Investigation of Clinically Relevant Fluconazole Resistance Mechanisms in the Fungal Pathogen Candida parapsilosis
… been shown to elevate the expression of MDR1 and CDR1/CDR2 respectively and directly contribute to fluconazole resistance in Candida albicans. Our lab previously identified three resistant isolates with upregulated CpMDR1 expression that contained CpMRR1 mutations, while CpTAC1 mutations were …
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Engineering and characterizing human T cell receptors for cancer immunotherapies
… called complementarity-determining regions (CDR1, CDR2, and CDR3), which interact with the peptide (pep)MHC antigen. The conserved docking angle of the six CDRs over the pepMHC antigens is thought to confer maximal signaling capabilities and it places the two most hypervariable loops (CDR3s) …
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Engineering and characterization of human single-chain T cell receptors
… the germline encoded regions of the TCR (i.e. CDR1 and CDR2) are positioned primarily over the MHC helices whereas the diverse regions derived from the junctions of somatically rearranged gene segments (i.e. CDR3) are positioned primarily over the peptide. Over the last 15 years, the Kranz lab …
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A 5-6 Ghz Silicon-Germanium Vco With Tunable Polyphase Outputs
… and fabricated using the Motorola 0.4 ìm CDR1 SiGe BiCMOS process, which has four interconnect metal layers and a thick copper uppermost bump layer for high-quality radio frequency (RF) passives. The VCO design includes full-wave electromagnetic characterization of an electrically …