Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"CDK9"”.
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Targeting CDK9 Reactivates Epigenetically Silenced Genes in Cancer
Cyclin-Dependent Kinase 9 (CDK9) as part of the PTEFb complex promotes transcriptional elongation by promoting RNAPII pause release. We now report that, paradoxically, CDK9 is also essential for maintaining gene silencing at heterochromatic loci. Through a live cell screen, we discovered that CDK9 …
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Investigating CDK9 inhibitor treatment during the innate inflammatory and regenerative response in a zebrafish model of cardiac injury
… influence downstream reparative mechanisms. CDK9 inhibitor compounds enhance the resolution of neutrophilic inflammation, however, their effects on cardiac repair/regeneration are unknown. Unlike adult mammalian hearts, zebrafish hearts regenerate following injury via cardiomyocyte …
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Epigenetic Regulation of Replication-Dependent Histone mRNA 3 End Processing
CDK9, eine cyclin-abhängige Ser/Thr-Kinase, phosphoryliert die C-terminale Domäne der größten RNA Polymerase II (RNAPII)-Untereinheit an Serin 2. Diese posttranslationale Modifikation reguliert die Aktivität der RNAPII im Anschluss an die Transkriptionsinitiation. Darüber hinaus wird CDK9 ebenfalls …
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STAT3-protein interactions in IL-6/gp130 signaling
… complex of STAT3 with cyclin-dependent kinase 9 (CDK9) was examined by using gamma-Fibrinogen (ã-FBG), an acute phase protein, as a model. IL-6 induces a strong nuclear association of STAT3 with CDK9, which is mediated via both STAT’s NH2-terminal and COOH-terminal domains. The induction of ã-FBG …
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Regulation and function of interphase histone H1 phosphorylation in pluripotent cell differentiation
… Selective inhibition of CDK7 and CDK9, or siRNA depletion of CDK9 rapidly diminishes both the global levels and the enrichment of pS187-H1.4 at housekeeping genes. Moreover, inhibiting transcription with actinomycin D induces the accumulation of pS187-H1.4 at promoters and gene …
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IDENTIFICATION OF CDC7 AS A NEW REGULATOR OF T CELL ACTIVATION
… assay, a dual inhibitor of the kinases Cdc7 and Cdk9, PHA-767491, was identified to inhibit various markers of T cell activation. PHA-767491 has an inhibitory effect on the TCR signalling pathway as Erk phosphorylation was suppressed. Furthermore, the data indicate that Cdc7 activity is increased …
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Transcriptional Elongation is Important for Neural Crest Development
… transcriptional elongation regulatory machinery, Cdk9 and CyclinT1 of the P-TEFb complex are also able to regulate neural crest specification. In particular the expression of the protooncogene c-Myc and c-Myc responsive genes are affected. c-Myc has been previously implicated in embryonic stem …
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Mechanisms of human papillomavirus and host gene transcriptional deregulation in cervical carcinogenesis
… enzyme (RNAPII-Ser2P), together with CDK9, the component of positive transcription elongation factor-b (P-TEFb) responsible for the Ser2 phosphorylation. The changes observed were functionally significant, as cells with higher HPV16 expression per template showed greater sensitivity to …
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Modulation of Host BRD4 to Repress HIV Replication in Myeloid Cells: Major HIV Reservoirs in Central Nervous System
… in microglia by disrupting binding of Tat to CDK9, a process key to HIV transcription elongation. High-resolution MNase mapping identified that ZL0580 induces repressive chromatin structure at the HIV LTR. Taken together, our data suggest that ZL0580 represents a potential approach that could …
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AN INVESTIGATION OF THE MECHANISMS OF GROUCHO MEDIATED TRANSCRIPTIONAL REPRESSION
… in Pol II pausing (including GAF, Nelf-E, Cdk9, and Myc), as well as Pol II peaks. It was also explored the co-localization between Gro and Psq, which together with GAF is known to bind to GAGA motifs, as well as with factors that associate with the two different Psq isoforms, containing …
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ATM REGULATES THE NF-kB PATHWAY VIA RELA SER 276 PHOSPHORYLATION
… complex containing cyclin dependent kinase-9 (CDK9; a kinase necessary for triggering transcriptional elongation) to promoters of NF-kB-dependent immediate early cytokine genes, in ATM knockdown cells. We conclude that ATM is a nuclear damage-response signal modulator of TNF-induced NF-kB …
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Site-specific phosphorylation of histone H1 associated with cell cycle progression and transcription
… of transcribed genes. Our data suggest CDK8 and CDK9, kinases with known association with the transcriptional machinery, as candidates that could account for this distribution of interphase H1 phosphorylation. Comparative analyses of H1 variant expression and phosphorylation in different human …
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Development of less toxic analogues of fascaplysin as novel therapeutic agents for the treatment of cancer
… Cdk2-cyclin A, Cdk2-cyclin E, Cdkl-cyclin B1 and Cdk9-cyclin Tl. The results from these assays led to the identification of compounds that specifically inhibit Cdk4 enzyme activity in vitro (i.e. compounds that are, at least, 10-fold more potent in inhibiting Cdk4 than Cdk2, Cdkl or Cdk9). …
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HETEROGENEITY OF LATENCY ESTABLISHMENT IN DIFFERENT HUMAN CD4+ T CELL SUBSETS STIMULATED WITH INTERLEUKIN-15
… T1 and phospho/Cyclin-dependent kinase 9 (P/CDK9). When we analyzed the cellular distribution of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) transcription factor, which is important for HIV expression, we observed that nuclear levels of NF-κB were lower in …
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Anti-cancer Functions and Mechanisms of a pRb2/p130 Peptide Fragment
… activities of Cdk1/Cyclin B, Cdk4/Cyclin D and Cdk9/Cyclin T/K. Result of a binding assay using GST-Spa310 and in vitro transcribed/translated Cdk2 did not support a direct binding between Spa310 and Cdk2. Additionally, GST-Spa310 was unable to bind to the in vitro transcribed/translated Cyclin …
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Fibroblasts: Key Cells in Inflammation and Fibrosis
… and ET domain containing protein 4) and CDK9 (cyclin dependent kinase 9) are needed for myofibroblast transformation. I further identify a small molecule inhibitor of BRD4, JQ1, which can not only prevent but also reverse the myofibroblast phenotype in cells taken from hypertrophic scars …
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The functions of the RNA polymerase II CTD in transcription and RNA processing
… and human cells, and cyclin-dependent kinase (CDK9) was likely the kinase to carry out this phosphorylation. We further provide evidence that Thr 4 functions in histone mRNA 3' end formation (presented mostly in chapter 2 of this thesis). Chapter 3 mainly describes the studies regarding Ser 2, …
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Significance of Protein Interactions in Mediating AF9 Function
… recruitment of cyclin dependent kinase 9 (CDK9), a component of P-TEFb and phosphorylation of Ser2 of C-terminal domain (CTD) of RNA PolII. Hence, our data suggest that peptide mediated disruption of AF9-AF4 interaction interferes with the stable complex formation involving P-TEFb, which …
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