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Showing 1 to 20 of 28 for “"CDC6"”.

  1. Effect of the cell-division cycle mutation cdc6-1 on chromosome structure and recombination in the yeast Saccharomyces cerevisiae

    McMillan, John N.East Carolina University2023

    ecu Repository record

  2. Characterization of the Protein Phosphatase 2A Regulatory Subunit PR70

    Davis, Anthony John2010

    … hybrid screen with the DNA replication protein Cdc6 as bait. PR70 interacts with the PP2A core dimer and Cdc6 in vivo and in vitro. Biochemical approaches were used to identify regions and residues within PR70 that are important for mediating protein-protein interactions with the PP2A core dimer …

    utswmed Repository record

  3. Lokalisation und Dynamik der Replikationsproteine des murinen prä-replikativen Komplexes

    Faul, ThomasUniversität Würzburg

    … konfokalen Laserscanning-Mikroskop untersucht. CDC6-EGFP war in der G1-Phase diffus in Zellkern und Cytoplasma verteilt, am G1/S-Übergang ausschließlich im Zellkern lokalisiert und während der S-Phase in zahlreichen Foci im Kern akkumuliert. CDC6-EGFP war mit Replikationsfoci colokalisiert. …

    wurz-thes Repository record

  4. Protein-Protein Interactions in Cell Cycle Proteins: An <i>In silico</i> Investigation of Two Important Players

    Eichner, AndrieleThe Graduate School and University Center of The City University of New York2024

    … cycle proteins, centering on two key players: Cdc6 in budding yeast and NEK10 in humans. In the first part of our study, we investigated Cdc6, an essential replication factor that binds origin recognition complex (ORC) and together these proteins work as a helicase loader. Regulation of Cdc6 is …

    cuny-grad Repository record

  5. Keep the ORCs at bay : how eukaryotic cells ensure one round of DNA replication per cell cycle

    Amasino, Audra Leigh.Massachusetts Institute of Technology2020

    … of the four helicase-loading proteins: Mcm2-7, Cdc6, and the Origin Recognition Complex (ORC). Phosphorylation of free Mcm2-7 and Cdc6 leads to their removal from the nucleus (Mcm2-7 by nuclear export and Cdc6 by protein degradation). However, phosphorylated ORC remains in the nucleus bound to …

    mit Repository record

  6. Single-molecule studies of eukaryotic helicase loading

    Ticau, SiminaMassachusetts Institute of Technology2016

    … involved in helicase loading are known (ORC, Cdc6, and Cdt 1), the mechanism by which they load two oppositely-oriented helicases and ensure their proper architecture remains under intense investigation. In this thesis I describe a novel single-molecule helicase-loading assay that allows …

    mit Repository record

  7. Analysis of cyclin-CDK mediated regulation of the G1/S transition in post quiescent murine fibroblasts

    Thacker, Urvi, Copeland, NikkiLancaster University2017

    … cells. CDK inhibition reduced levels of CDC6, MCM2 and PCNA in all contexts. Evaluation of the role of individual cyclin-CDK complexes was performed by siRNA mediated depletion. Cyclin E1/E2 depletion showed that Cyclin E regulates CDC6, MCM2 and PCNA at the transcriptional level; whereas …

    lancaster Repository record

  8. Interaktionen der Replikationsproteine der Maus

    Jehle, MargotUniversität Würzburg

    … Origins ausgelöst wird. Anschließend lagern sich CDC6- und RLF-B/CDT1-Protein an den ORC an, die beide schließlich für die Rekrutierung des heterohexameren MCM-Komplexes verantwortlich sind. Durch die Aktivität der CDC7/DBF4-Kinase wird der Origin lizensiert, nachdem der letzte Initiationsfaktor …

    wurz-thes Repository record

  9. Sequenz-spezifische Interaktion des murinen präreplikativen Komplexes mit Origin-DNA

    Herrmann, ThomasUniversität Würzburg

    … und Geminin von Fragment B ablösen, während sich CDC6 und -45 an den ORC-Komplex anlagern. Mit Erreichen der S-Phase konnte gezeigt werden, dass sich CDC6 und -45 sowie ORC1 wieder ablösen und ein Core-Komplex von ORC2-5 sowie MCM3 und -6 gebunden bleibt. Außerdem konnte an Fragment B eine …

    wurz-thes Repository record

  10. Role of Chk1 and Chk2 in mitotic checkpoint control in vertebrate cells

    Oehler, VerenaUniversity of Glasgow2008

    … Furthermore, this study revealed that endogenous Cdc6 accumulates in a Chk1-dependent manner during replication arrest. To test whether Cdc6 might function upstream or downstream of Chk1 in the replication checkpoint pathway, Cdc6 was ectopically expressed in both checkpoint-proficient and …

    glasgow Repository record

  11. An ORC Flip Enables Bidirectional Helicase Loading

    Gupta, ShaliniMassachusetts Institute of Technology2022

    … recognizes and binds origin DNA and recruits Cdc6. Mcm2-7 in complex with a third protein Cdt1 associates with ORC/Cdc6/DNA. Cdc6 and Cdt1 are sequentially released to allow loading of an oppositely-oriented second Cdt1-bound-Mcm2-7 hexamer that forms the Mcm2 7 double hexamer. Multiple …

    mit Repository record

  12. Funktionelle Analyse der Interaktion und Lokalisation von Replikationsfaktoren und replikationsrelevanten Proteinen in Mauszellen

    Auth, TanjaUniversität Würzburg

    … 1a mit den Replikationsfaktoren ORC1, ORC2 und CDC6 sowohl in Two Hybrid-Studien als auch in Immunpräzipitationen gezeigt werden. Darüberhinaus konnten signifikante Kolokalisationen dieser Proteine mit HP1a an heterochromatischen Regionen in murinen NIH3T3-Zellen nachgewiesen werden. Ebenfalls …

    wurz-thes Repository record

  13. Wechselwirkungen zwischen Replikationsproteinen und Origin-DNA während Proliferation und terminaler Differenzierung

    Zellner, ElisabethUniversität Würzburg

    … gegen Replikationsproteine untersucht ORC1, CDC6, MCM6 und Geminin konnten nach 132 h nicht mehr detektiert werden, während ORC2, ORC3, MCM3, CDT1 und CDC45 zwar noch vorhanden waren, jedoch in geringerer Menge als in proliferierenden Zellen. Weiterhin wurde die Menge an Replikationsproteinen …

    wurz-thes Repository record

  14. Inhibition of Hox function by the cell cycle regulator geminin

    Luo, Lingfei2012

    … während des Zellzyklusses, durch ORC, Cdc6, Cdt1 und den MCM-Komplex kontrolliert. Die Beteiligung von Geminin an diesen beiden Prozessen ist kompetetiv. Durch die Etablierung dieser kompetetiven Regulation fungiert Geminin als Koodinator zwischen Entwichlungs- und …

    goettingen Repository record

  15. Temporal organization of the budding yeast cell cycle: general principles and detailed simulations

    Calzone, LaurenceVirginia Tech2003

    … with stoichiometric inhibitors (Sic1, Cdc6); their degradation by SCF and adaptors of the APC (Cdc20, Cdh1). The emphasis was put on mechanisms responsible for the release of Cdc14 from the RENT complex, Cdc14 being a major player in exit from mitosis. Simulations of the wild type …

    vt Repository record

  16. Lokalisation, Regulation und Interaktionen muriner DNA-Replikationsproteine

    Brand, NormenUniversität Würzburg

    … Mcm2 7 komplettiert in Abhängigkeit von Cdc6 und Cdt1 den prä-replikativen Komplex (pre-RC) und wird vermutlich nach der Initiation vom Origin entfernt, um als DNA-Helikase für die Entwindung der DNA-Doppelhelix zu sorgen. Dies ermöglicht den Proteinen der Elongations-Maschinerie DNA an …

    wurz-thes Repository record

  17. Origins of non-coding Y RNA family and its role in DNA replication

    Pavlickova, MilenaUniversity of Cambridge2021

    … I showed that replication factors such as ORC, Cdc6 and Cdt1 are unlikely to interact with the Y RNA upper stem domain, contrary to previous findings. Functional homologs of Y RNAs called sbRNAs, have been identified in the nematode genus *Caenorhabditis*. However, no additional Y RNAs were …

    cambridge Repository record

  18. Mechanisms preventing DNA replication between Meiosis I and Meiosis II

    Phizicky, David V. (David Vincent)Massachusetts Institute of Technology2018

    … to transcriptionally and proteolytically repress Cdc6, an essential helicase-loading protein. In addition, I found that meiotic cells use CDK and the polo-like kinase Cdc5 to promote degradation of Sld2, an essential helicase-activation protein. Together, these data demonstrate that multiple …

    mit Repository record

  19. Impact of DNA accessibility and sequence on eukaryotic origin licensing

    Warner, Megan D. (Megan Dobbins)Massachusetts Institute of Technology2017

    … proteins, the origin-recognition complex (ORC), Cdc6, and Cdtl, as well as the DNA sequence present at potential origins. Although in S. cerevisiae, ORC binds to a known DNA sequence, where the other proteins, particularly the Mcm2-7 helicase, interact with DNA is not known. To gain additional …

    mit Repository record

  20. Molecular Characterization of Mth203 protein

    Bochiwal, CUniversity of York2011

    … It’s replication initiation proteins MthCdc6-1 and MthCdc6-2 are homologues of the eukaryotic Cdc6 and ORC proteins. It also contains a single minichromosome maintenance protein (MthMCM) homologue which forms a hexameric complex and acts as a DNA helicase. What loads the archaeal MCM …

    whiterose Repository record

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