Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 21 for “"CD9"”.
-
Die Rolle des CD9 bei der CDV-Infektion
… dieser Fusionsprozesse. Kürzlich konnten wir mit CD9, einem Mitglied der Tetraspann-Transmembran-Proteinfamilie, ein zelluläres Oberflächenmolekül identifizieren, welches bei einer Infektion von Zellen mit CDV an diesen Fusionvorgängen beteiligt ist: Transfektion eines CD9-Expressionsplasmids in …
-
Molecular Mechanism of Tetraspanin CD9 Mediated Cell Motility
<p>CD9, a member of the tetraspanin superfamily of proteins participates in the regulation of cell adhesive functions such as cell migration. The mechanisms underlying CD9 mediated cell migration are not known. In the current study, we investigated the molecular basis for the CD9 promoted cell …
-
The Tetraspanin CD9 Localizes to Platelet-Platelet Contacts and Regulates Thrombus Stability
<p>CD9, a member of the tetraspanin family, is highly expressed on platelets (50,000-80,000 copies per platelet). Tetraspanins have been implicated in modulation of integrin function, and it is hypothesized that CD9 will modulate GPIIb-IIIa, the major platelet integrin.</p> <p> The association …
-
Tetraspanin CD9: Contributions to Cancer Cell and Vascular Cell Phenotypes from the Sixth Man
… signaling. This work examines tetraspanin CD9 contributions in a model of cancer cell invasion and vascular cell contraction. The findings presented here demonstrate that CD9 expression promotes human fibrosarcoma cell invasion by upregulating matrixmetalloproteinase-9 expression. These …
-
Investigations of tetraspanin functions using large extracellular loops
… of a monoclonal antibody against the tetraspanin CD9 serves to inhibit fertilization, consistent with the CD9-null mouse model. The first area of focus in this thesis is the characterization of the murine CD9-LEL domain. We present a methodology to express and purify the mCD9-LEL to homogeneity. …
-
Expression of Tetraspanin Markers in Benign Prostate Hyperplasia and Prostate Cancer // Експресия на тетраспанинови маркери при доброкачествена хиперплазия и карцином на простатната жлеза
… тумори. Изследването на тетраспанините CD9 и CD151 би довело до разкриване ва зависимостта между тези протеини и някои от най-неблагоприятните клиникоморфологични параметри при простатния карцином като периневрална инвазия, ниска туморна диференциация и крибриформен модел на растеж. …
-
Use of artificial neural network techniques to model proteomics of cellular stress
… used to predict the levels of surface markers CD9 CD147 and HLA A-C based on experimental parameters of data obtained from ultra-scaled down experiments. These models where used to predict how changing the parameters would affect the density and abundance of the surface markers on the cell …
-
Digital Profiling of Circulating Extracellular Vesicles at Single-Upconversion Nanoparticle Sensitivity and Resolution
… we apply UCNPs for direct enumeration of single CD9 and EpCAM positive EVs (CD9+EpCAM+EVs). The achieved single-molecule sensitivity results in a femtomolar detection limit (1.8 × 106 EVs mL-1), which was nearly 3 orders of magnitude lower than the standard enzyme-linked immunosorbent assay …
-
Regulation of Hematopoietic Stem Cells
… we have identified the cell surface antigen CD9 as a positive marker for HSCs that provides a simple alternative for stem cell isolation at high purity. Using CD9 as a tool we have dissected heterogeneity within the HSC pool as defined by CD9 expression (Article III). Taken together, we have …
-
Obesity-Induced Alterations in Sperm Quality, Testicular Morphology, and Extracellular Vesicle Localisation in Male Mice
… EV-specific biomarkers such as CD63, CD9 and ANXA1 were targeted. Frozen testes were utilised for EV extraction and further western blotting (WB) analyses. Spermatogenesis was investigated via WB for spermatogenesis-related proteins (IGF1, INSL6, and PRDX2). For EV extraction, frozen …
-
Development of a multi-stage purification process for serum-derived exosomes and evaluation of their regenerative capacity
… suitably sized vesicles and exosomal markers (CD9, CD81 and TSG101). These isolates allowed for cellular uptake of a range of fluorescent labels and enhanced cellular fibroblast proliferation and endothelial sprout formation in a 3D spheroid-based angiogenesis assay. Further to this, …
-
Analysis of the Role of Piwil2 gene in Tumorigenesis and Germline Stem Cell Metabolisms
… spermatogonialen Zelloberflächenmarker Thy-1 (CD90), Itga6 (Integrin alpha 6), CD9 und die spermatogonialen Marker Hsp90α (Heat shock protein 90 alpha) und Stra8 (Stimulated by retinoic acid gene 8). Diese Moleküle spielen eine essentielle Rolle bei der Stammzellproliferation (Pdgfrb), dem …
-
Integrated Microfluidic System for Digital Detection of Extracellular Vesicles
… EVs. A cocktail mixture of biotin-labeled anti-CD9 and anti-CD63 antibodies were used as detection antibodies to detect the captured EVs. Enzymatic amplification of the detection signal was achieved with the help of Streptavidin Beta Galactosidase (SßG) as a reporter enzyme and …
-
Isolation und Charakterisierung extrazellulärer Vesikel aus humanen CD8+- und γδ-T-Lymphozyten
… EV-spezifischer Marker, u.a. der Tetraspanine CD9 und CD81 sowie HSP70, Syntenin-1, TSG101 und ALIX. Insgesamt ergab sich ein weitgehend homogenes Spektrum an Proteinen in EV beider T-Zellpopulationen. Die Gen-Ontologie-Bewertung ergab eine deutliche Anreicherung von Proteinen, die mit …
-
Regulation of C-type lectin-like receptors dectin-1 and CLEC-2 by tetraspanins
… and deletion of the major platelet tetraspanin, CD9, did not affect dectin-1 and CLEC-2 signalling, respectively. In summary, the importance of transient tetraspanin over-expression for dectin-1 and CLEC-2 inhibition, and the fact that any tetraspanin can inhibit, suggests that tetraspanin …
-
Microfluidic-based Fluorescence Detection for Biomarker Analysis
… (3 common tetraspanin markers, including CD9, CD63 and CD81 and epidermal growth factor receptor (EGFR)). Based the colocalization of fluorescence signals from protein markers and membrane dye, antibody-labeled exosomes can be clearly differentiated from free antibodies. This platform can …
-
Impact of chemotherapy on the release and function of extracellular vesicles in the pre-pubertal testis
… EVs were assessed for common EV surface markers CD9, CD63 and CD81 using dSTORM imaging and transmission electron microscopy (TEM) while bulk assessment of EVs for another common EV marker, TSG-101, was undertaken using Western blotting. Assessment of EV release and impact of cisplatin-induced …
-
Isolation und molekulare Charakterisierung des Apoptose-induzierenden Metastasensuppressors C33/CD82
… angehört, Apoptose induzieren: hierzu zählen CD9, CD37, CD81 und CD151 und in geringerem Umfang auch CD53 und CD63. Mit Hilfe trunkierter Mutanten wurde der für die Apoptoseinduktion verantwortliche Bereich auf die ersten 119 Aminosäuren von C33/CD82 eingegrenzt. Nach den Ergebnissen dieser …
-
Identification of exosomal proteins in primary human bronchial tracheal epithelial cell HBTE and the H358, THP1 and MCF7 cell lines
… by the presence of exosomal markers (CD63, CD81, CD9 and Hsp70). The number of exosomes released/cell was shown to increase with time ranging from 14500 on day 1 to 18000 at day 15. Interestingly, MS analysis revealed that, alpha-2-macroglobulin (A2M) and pregnancy zone protein was present only in …
-
THE ROLE OF EXTRACELLULAR VESICLES IN HEART DISEASES
… and immunophenotyping for surface markers (e.g. CD9, CD63 for tetraspanins; CD14 for monocytes; CD206 for macrophages; CD202b for endothelial cells; CD172a for cardiomyocytes; CD66b for neutrophils; CD41a for total platelets). For targeted (Olink) and untargeted (HPLC-MS/MS) proteomic profiling, …
Page 1 of 2