Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 28 for “"CD81"”.
-
Autophagy and EVs in cancer cells. Effect of ATG8 knockdown on the biogenesis of CD81+ extracellular vesicles.
… conditioned medium were immunocaptured with CD81 Fab-Strep. After isolation, the particles were analyzed with NanoSight for particle concentration and size, immunoblotted for protein markers with SW and imaged with TEM. This study determined that the EV secretion from ATG8 hexa-KO cell lines …
-
Molecular Analysis of Hepatitis C Virus Envelope Glycoprotein E2 Binding and Entry
… to the cluster of differentiation 81 receptor (CD81) and to the scavenger receptor class B type 1 (SR-B1). The critical incorporation of claudin-1 receptor (CLDN-1) and occludin receptor (OCLN) (indirect interaction) has been reported. Then, fusion process is intiated between viral and cellular …
-
The Structural Basis of Flaviviridae Interaction with Antibodies and Receptors
… E2 mediates interaction with host receptors CD81 and scavenger receptor BI: SR-BI) and is the primary target of neutralizing antibodies. To elucidate detailed biochemical roles for these receptors' interactions with E2, we determined that the E2 ectodomain: sE2) interacts with soluble CD81 …
-
The role of scavenger receptor B-I in hepatitis C virus attachment and entry.
… on at least three cellular entry factors: CD81, Scavenger Receptor B-I (SR-BI) and Claudin-1. SR-BI is a receptor for high density lipoprotein (HDL), it is predominantly expressed in the liver and steroidogenic tissue. HCV is believed to interact with SR-BI via the viral envelope protein …
-
Isolation und Charakterisierung extrazellulärer Vesikel aus humanen CD8+- und γδ-T-Lymphozyten
… in den EV-Proben die Tetraspanine CD63 und CD81 als EV-Marker nachgewiesen, ebenso das zytotoxische Effektorprotein Granulysin. Um die Proteinzusammensetzung der EV zu untersuchen erfolgte eine vergleichende Proteomanalyse mittels Tandem-Massenspektrometrie. Dabei konnten über 900 Proteine …
-
Genetic Networks Modulating Retinal Injury
… whereas, glial reactive markers such as Gfap and Cd81 define the sustained response. Second, three specific genomic loci modulate coordinated changes in gene expression in mouse brains: regulatory loci on chromosomes 6, 12, and 14. Of the three only the regulatory locus on chromosome 12 …
-
Caractérisation morphologique et moléculaire des vésicules extracellulaires libérées par des cellules musculaires primaires humaines provenant d'individus sains et les personnes atteintes de diabète de type 2
… pour les marqueurs vésiculaires TSG101, CD63, CD81. Parmi les méthodes d’isolement testées, ExoQuick et EasySep se sont révélés être plus performants en termes de rendement et de pureté avec respectivement 4,2.10¹⁰ particules/mL et 4,3.1013 particules/mL et une absence de calnexine dans les …
-
Developmental Expression of Rat Target of the Antiproliferative Antibody (rTAPA) Protein in the Brain
… study defines the expression pattern of rTAPA (CD81) in the developing rat brain. rTAPA is a member of the tetramembrane spanning family of proteins, and like other members of this family, appears to be associated with the stabilization of cellular contacts. On immunoblots of the brain, rTAPA is …
-
Extracellular matrix protein receptors in Drosophila melanogaster
… to by their vertebrate names, and include CD81, a member of the tetraspanin family, ILK, integrin-linked kinase, and CD98hc, a type II transmembrane glycoprotein which is the heavy chain of a multi-protein complex. In this work, the mutant phenotype of CD98hc is larval lethal and not …
-
Integrated Microfluidic System for Digital Detection of Extracellular Vesicles
… the detection area of the chip coated with anti-CD81 antibodies are used to capture EVs. A cocktail mixture of biotin-labeled anti-CD9 and anti-CD63 antibodies were used as detection antibodies to detect the captured EVs. Enzymatic amplification of the detection signal was achieved with the help …
-
Defining the mechanism(s) of Hepatitis C virus (HCV) entry
… is clathrin- and pH-dependent, and requires CD81, Scavenger receptor BI (SR-BI), and the tight junction (TJ) proteins Claudin-1 and Occludin. Primary HCV strains cannot be efficiently cultured in vitro, making the evaluation of potential antiviral compounds in a biologically relevant context …
-
Characterizing B Cell Phenotype During Chronic HCV Infection
… require viable virions, but was dependent upon CD81 availability and the presence of monocytes. We also determined that upon viral exposure, these B cells replicated the hyper-activation of MLR responses found in ex-vivo derived B cells from chronic HCV individuals. In all, our results suggest a …
-
Dissecting the Entry Pathways of Hepatitis C and Bovine Viral Diarrhea Viruses
… The role of the cellular receptors/coreceptors, CD81, scavenger receptor class B type 1 (SR-BI), and claudin-1 (CLDN1), in mediating HCV entry and their modulation during persistent HCV replication were also investigated. This work highlights common features shared between BVDV and HCV and …
-
Regulation of C-type lectin-like receptors dectin-1 and CLEC-2 by tetraspanins
… knockdown of the major Jurkat cell tetraspanin, CD81, and deletion of the major platelet tetraspanin, CD9, did not affect dectin-1 and CLEC-2 signalling, respectively. In summary, the importance of transient tetraspanin over-expression for dectin-1 and CLEC-2 inhibition, and the fact that any …
-
INVESTIGATING THE ROLE OF EXTRACELLULAR VESICLES AND GLYCOSYLATION IN CANCER METASTASIS
… increased lectin binding of LCA and TL in ‘CD81-positive’ sEVs derived from breast cancer-associated cell lines in comparison to normal cells. Validation through single-vesicle flow cytometry confirmed these observations, prompting investigations into the diagnostic application of these …
-
Investigating the role of extracellular vesicles and glycosylation in cancer metastasis
… increased lectin binding of LCA and TL in ‘CD81-positive’ sEVs derived from breast cancer-associated cell lines in comparison to normal cells. Validation through single-vesicle flow cytometry confirmed these observations, prompting investigations into the diagnostic application of these …
-
Microfluidic-based Fluorescence Detection for Biomarker Analysis
… tetraspanin markers, including CD9, CD63 and CD81 and epidermal growth factor receptor (EGFR)). Based the colocalization of fluorescence signals from protein markers and membrane dye, antibody-labeled exosomes can be clearly differentiated from free antibodies. This platform can characterize …
-
Investigations into the dynamic uptake of extracellular vesicles and their role in breast cancer metastasis after chemotherapy
… a fluorescently tagged EV marker (mEmerald-CD81) were preferential for experiments investigating EV dynamics, as labelling appeared to be more specific than the dyes previously tested. Using this type of labelled EVs, it was shown that EV uptake differs across cell lines and the EV origin …
-
Investigating the role of TRPC3 in the biogenesis of extracellular vesicles
… EVs revealed previously unappreciated roles for CD81, NDPKs and Ca2+-dependent interactions with the syndecan-syntenin-ALIX axis in EV biogenesis. Proteomic analysis also evidenced the endosomal origin and enriched metastatic potential of TRPC3 activator-induced EVs, as well as presenting novel …
-
Investigation of Cancer Stem Cells (CSCs)-derived exosomes and their influence on the tumor microenvironment
… to well known markers, such as Tsg101 and CD81, LCSC-derived exosomes are enriched in CD133 protein. Immunoprecipitation (IP) experiments revealed that by immunoprecipitating the culture SN of CD133 expressing cells with an antibody against CD133 protein, typical exosomal markers, such as …
Page 1 of 2