Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"CD64"”.
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Entwicklung, Optimierung und Charakterisierung anti-CD64-basierter Immuntherapeutika
… often characterized by overexpression of FcγR I (CD64), a receptor with high affinity for the Fc portion of immunoglobulins G1 and G3, which is normally expressed on monocytes and macrophages. These AML cells are targeted efficiently by the humanized anti-CD64 single chain antibody fragment (scFv) …
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Herstellung und Charakterisierung von rekombinanten, ETA-basierten CD64-Immuntoxinen zur Bekämpfung von dysregulierten Makrophagen bei chronischen Hautentzündungen
… fact that a selective elimination of the high CD64 expressing macrophages, through CD64 immunotoxin (full length antibody with a toxin), results in a resolution of chronic inflammations, confirms this. The immunotoxins of the first generation cannot be produced (however) in homogeneous quality …
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Generation and therapeutic evaluation of recombinant immunotherapeutics on CD64+ monocytic cell lines and polarized ex vivo differentiated human macrophages
… have identified Fc gamma receptor I (FcγRI)/CD64 as a significant therapeutic target. CD64 is a transmembrane surface receptor found exclusively in myeloid lineage cells such as monocytes and different macrophage subtypes. Hence, protein engineering and biotechnology techniques were used to …
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Augmenting Natural Killer cell antibody binding to enhance ovarian and prostate tumor cell killing via ADCC
… recombinant FcγR, referred to here as CD64/16A. CD64/16A consists of the extracellular region of CD64, the highest affinity IgG FcγR, and the transmembrane and intracellular regions of CD16A. We expressed CD64/16A in human NK-92 cells, a cell line that lacks expression of endogenous …
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Point-of-care microfluidic assays for measuring expression level of antigen on blood cells
Elevated expression of a membrane protein CD64 on neutrophils is linked with the onset of sepsis, a life-threatening syndrome that contributes to millions of deaths annually worldwide. The survival rate of septic patients falls rapidly every hour the appropriate medication is delayed. Therefore, …
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A microfluidic biosensor to electrically enumerate blood cells at point-of-care for infectious disease diagnosis and management
… application. Increased expression of the CD64 antigen on the neutrophils is a biomarker for early diagnosis of sepsis. However, to use our technology to count CD64 neutrophils, as a first step, using blood samples collected from patients admitted to ICU we have shown a high correlation of …
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Construction and characterisation of anti-tumour antibody-based proteins for use in diagnostics and therapeutics
… specificity towards the immune cell receptor CD64 and NIP. Successful DuoBody formation revealed the capacity of these biMabs to bring CD64-expressing immune cells and target cells (in this case derivatised with the NIP antigen) into close proximity for immune-mediated cell destruction. …
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Regulation of Human Monocyte Function by Cross-Linking of Immunoglobulin E
… of an important mediator of phagocytosis, CD64. Indeed, IgE cross-linking specifically impairs monocyte phagocytic function without disrupting the capacity of monocytes to kill intracellular bacteria. IgE cross-linking also reduces expression of several surface molecules associated with …
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Expression and characterisation of biopharmaceuticals in heterologous expression systems
… receptor for human IgG, Fc-gamma-RI (CD64), were successfully established and optimised for heterologous expression in plants, bacteria and mammalian. The first aim of this thesis was the establishment of a protocol for the expression and purification of the anti-HIV antibody, 2F5, in …
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Molekulare Mechanismen der antiinflammatorischen und immunsuppressiven Effekte von Interleukin-10
… Wirkung von IL-10 auf den Fc?-Rezeptor CD64, der eine Rolle bei der Phagozytose spielt, konnte gezeigt werden, dass HDAC1 essentiell ist. Die Ergebnisse tragen zum besseren Verständnis der IL-10-Signaltransduktion bei. Neben der Untersuchung der IL-10-Signaltransduktion wurde den …
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Targeting the Tumor Stroma Using a Monoclonal Antibody Platform Technology
… agent in combination with an engineered NK-92MI CD64 cell therapy. The immunotherapy demonstrated selective cytotoxicity in vitro and treatment effectively controlled tumor growth in an animal model. Furthermore, we engineered B12 mAb as an antibody-drug conjugate (ADC) and showed cytotoxic …
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Role of monocyte subsets in chronic liver injury and liver fibrosis
… monocyte CD14++CD16- subset expresses CCR2, CD64, CD62L and low levels of the fractalkine receptor CX3CR1, whereas the non-classical CD14lowCD16+ monocyte subset expresses high levels of CX3CR1 and lack CCR2. Their counterparts in mice are CCR2+Gr-1hi and CCR2-Gr-1low monocytes, respectively. …
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Erweiterung eines diagnostischen Verfahrens zur Differenzierung entzündlicher Dermatosen mittels Immunphänotypisierung dendritischer Zellen der Epidermis
… So konnte die Psoriasis durch eine Ratio von CD64/CD11b auf IDEC sinnvoll abgegrenzt werden. Das Kontaktekzem war mittels einer Ratio aus E-Cadherin/CD86 auf Langerhans Zellen zu identifizieren. Die Gruppe der Patienten mit Kontaktekzem bei atopischer Diathese wurde durch die Ratio …