Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"CD63"”.
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Analysis Of The Structure And Function Of A Timp-1/cd63 Complex And Its Relationship To An Mt1-Mmp/cd63 Complex
… assay (PCA) of the interaction of TIMP-1 and CD63. CD63 is a tetraspanin, whose major functional properties are not well understood. We have previously identified CD63 as a non-MMP protein and a transmembrane (TM) protein with which TIMP-1 has direct interaction, resulting in intra-cellular …
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The role of tetraspanin pretein CD63 in humna immunodeficiency virus type 1 infection and replication
… Previous works demonstrated a role for CD63 in early HIV infection events in macrophages via inhibition by pretreatment of an anti-CD63 antibody, which did not inhibit HIV replication in peripheral blood lymphocytes or in two CD4+ cell lines tested. To confirm the requirement for CD63 in …
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Untersuchungen zur Expression der Oberflächenmarker CD63 und CD203c basophiler Granulozyten bei Bienen- und Wespengiftallergikern mit Hilfe des Basophilen Aktivierungstestes (BAT)
… der membranständigen Aktivierungsmarker CD63 und CD203c vor und nach Antigenexposition gemessen. CD63 befindet sich intragranulär gespeichert in ruhenden basophilen Granulozyten, Mastzellen, Makrophagen und Monozyten. In vitro konnte eine verstärkte Expression von CD63 v.a. …
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TIMP-1 Activates a Unique Cardiac Stem Cell Population, CD63+ve/C-KIT+ve, Thereby Enhancing Cardiac Differentiation, and Protects the Heart From Adverse Cardiac Remodeling Following Myocardial Infarction
… data validate the existence of a novel CD63+ve/c-kit+ve cells. When treated with TIMP-1, these cells showed significantly (p < 0.05) increased proliferation and differentiation into cardiac myocytes, vascular smooth muscle cells, and endothelial cells. Western blot analysis revealed …
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Autoreaktyvumo rodiklių diagnostinė reikšmė sergant lėtine dilgėline /
… of CU patient’s serum to evoke expression of CD63 and CD203c on donor basophils by using flow cytometry is showing some promise. The aim of our study was to evaluate the diagnostic significance of autoreactivity markers and their correlation in chronic urticaria patients. Sera from patients …
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Autoreaktyvumo rodiklių diagnostinė reikšmė sergant lėtine dilgėline /
… of CU patient’s serum to evoke expression of CD63 and CD203c on donor basophils by using flow cytometry is showing some promise. The aim of our study was to evaluate the diagnostic significance of autoreactivity markers and their correlation in chronic urticaria patients. Sera from patients …
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Novel findings on cellular trafficking and targeting for granule storage of neutrophil elastase, a multifunctional effector molecule of innate immunity
… was demonstrated between the tetraspanin CD63 and proNE upon coexpression in COS cells. Furthermore, depletion of CD63 in a promyelocytic cell line (achieved by RNA interference or a CD63 mutant) caused reduced processing of proNE into mature NE and reduced constitutive secretion of proNE. …
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A novel function of tissue inhibitor of metalloproteinase-1 as a chemoattract molecule for neural stem cells
… cells do not. After then, to investigate if CD63, one of TIMP-1 membrane receptors, mediate NSC migration by TIMP-1 on the cell surface, we established CD63-knockdown F3 cell lines using a vector-based small hairpin RNA (shRNA) strategy. Interestingly, CD63 knockdown blocked almost all NSC …
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Effector Cells and Mechanisms in Chronic Spontaneous Urticaria
… peripheral blood basophils (CCR3+CD123+ vs CCR3+CD63+ (p=0.0001), CD63+CD203c+ vs CCR3+CD123+ (p=0.0003), CD63+CD203c+ vs CCR3+CD63 (p=0.0001)). We then examined basophil variation using ImageStream® in healthy subjects. ImageStream® studies confirmed the difference in basophil percentages …
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Obesity-Induced Alterations in Sperm Quality, Testicular Morphology, and Extracellular Vesicle Localisation in Male Mice
… respectively. EV-specific biomarkers such as CD63, CD9 and ANXA1 were targeted. Frozen testes were utilised for EV extraction and further western blotting (WB) analyses. Spermatogenesis was investigated via WB for spermatogenesis-related proteins (IGF1, INSL6, and PRDX2). For EV extraction, …
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Signalbindung und Membraninteraktion von heterotetrameren Adaptorprotein-Komplexen
… lysosomalen Proteine LAMP-1, LAMP-2A, LAMP-2B, CD63 und des am TGN-lokalisierten Proteins TGN38 mittels Oberflächenplasmonresonanz-Analyse (Biacore) in vitro untersucht. Alle getesteten Signale interagierten kaum mit AP4, aber gut mit AP3. Die Bindung an AP1 und AP2 war deutlich heterogener. Am …
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Comprehensive Proteomic Analysis and Characterization of Human Bone Marrow Mesenchymal Stem/Stromal Derived Extracellular Vesicles
… identified EVs expressing exosomal tetraspanins (CD63/81/9) and western blot analysis confirmed an enriched expression of MMP-2 and HSP90B1 in hBM-MSC-EVs. Quantitative proteomic analysis performed using Tandem Mass Tag labeling combined to LC-MS/MS identified 5108 proteins in parental hMSCs …
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The Tetraspanin CD9 Localizes to Platelet-Platelet Contacts and Regulates Thrombus Stability
… binding and platelet activation (as evidenced by CD63 and P-selectin expression) were elicited in the presence of threshold ADP levels. Both responses were dependent on presence of low-level agonist, as CD9 perturbation in the absence of agonist yielded no effect. </p> <p> Cytoskeletal isolation …
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Characteristic analysis of mobility inducers for neural stem cells
… CM-2 induced NSCs migration by interacting with CD63 and CM-2-CD63complex bound to integrin β1. To investigate the detailed molecular mechanism for NSCs migration by CM-2, in migration assay, we confirmed that integrin β1 and PI3K signaling pathways were implicated in the induction of NSCs …
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Contributions of type 2 IP3 receptors to intercellular adhesions and invasion of glioblastoma cells
… mRNA for TGFBR2 and decreases for Connexin43 and CD63. I then assessed six candidates ZDHCC9, Connexin43, CD63, N-cadherin, Cadherin11 and TGFBR2 identified by the unbiased analyses in functional analyses of invasion and intercellular adhesion. Using siRNA against each of these candidates, I …
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Identification of exosomal proteins in primary human bronchial tracheal epithelial cell HBTE and the H358, THP1 and MCF7 cell lines
… stages using Exo-Elisa based on exosomal marker CD63 and mass spectrometry (MS) respectively. LC-MS based proteomic approach has been used to compare the proteomic profile of exosomes from three cancer cells. Finally, comparative proteomic study and gene expression analysis were carried out …
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A novel principle for immunomodulator delivery with hematopoietic cells as vehicles Focus on soluble TNF-receptor gene expression, targeting to secretory lysosomes and regulated secretion
… cytosolic sorting signal (Y) taken from CD63. This signal directed sTNFR1-tm-Y from the trans-Golgi network (TGN) to secretory lysosomes, followed by generation of membrane-free soluble sTNFR1. The secretion of sTNFR1 could be triggered by a calcium-ionophore or by crosslinking of the IgE …
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TIMP-1 as a Modulator of Inflammatory Pain
… We found that peripheral knockdown of CD63, a receptor for TIMP-1, was sufficient to promote spontaneous inflammatory pain and increase expression of Nav1.7 mRNA in the DRG. These findings define a mechanism of TIMP-1-mediated attenuation of inflammatory hypersensitivity and suggest …
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Integrated Microfluidic System for Digital Detection of Extracellular Vesicles
… mixture of biotin-labeled anti-CD9 and anti-CD63 antibodies were used as detection antibodies to detect the captured EVs. Enzymatic amplification of the detection signal was achieved with the help of Streptavidin Beta Galactosidase (SßG) as a reporter enzyme and …
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Isolation und Charakterisierung extrazellulärer Vesikel aus humanen CD8+- und γδ-T-Lymphozyten
… Blot wurden in den EV-Proben die Tetraspanine CD63 und CD81 als EV-Marker nachgewiesen, ebenso das zytotoxische Effektorprotein Granulysin. Um die Proteinzusammensetzung der EV zu untersuchen erfolgte eine vergleichende Proteomanalyse mittels Tandem-Massenspektrometrie. Dabei konnten über 900 …
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