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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 77 for “"CD45-"”.
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Combinatorial Regulation of Signal-Induced CD45 Exon Repression by hnRNP L and PSF
CD45 is a hematopoetic-specific tyrosine phoshatase. In resting T cells three variable exons are partially repressed, and following antigen challenge, these exons are more highly repressed. Previous work identified the ESS1 silencer element that functions to mediate exon 4 silencing under resting …
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Formation of an Exon-Defined A Complex Spliceosome Intermediate Results in CD45 Exon Repression
… while maintaining accurate splicing fidelity. CD45 is a trans-membrane tyrosine phosphatase that is expressed on the surface of hematopoetic cells. The CD45 gene contains three variable exons that are partially repressed in na?T cells and are preferentially skipped upon T cell activation. …
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Molecular Mechanisms of Alternative Splicing Regulation: An Investigation of the Spliceosome Repressed by Hnrnp L on Cd45 Exon 4
… of splice site choices. In our lab, we used CD45 as a model gene for studying alternative splicing and spliceosome assembly. The exonic silencer sequence (ESS1) within CD45 exon 4 is bound by hnRNP L to induce its skipping. HnRNP L represses spliceosome assembly at a step after the binding of …
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Bone Marrow Derived Progenitor Cells and Their Contributions to the Thymic Stroma
… that co-express the hematopoietic marker CD45 and the definitive thymic epithelial marker EpCAM, which is also highly enriched in stem cell markers such as, Sca1. CD45+ EpCAM+ cells are present in both the thymus and bone marrow (BM), suggesting that the CD45+EpCAM+ population may be …
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Describing the immune cell profile of ventricular cerebrospinal fluid (CSF) in paediatric central nervous system (CNS) infections
… populations of major peripheral immune cells (CD45+: lymphocytes, monocytes and granulocytes) and CNS-derived immune cells (microglia (CD45-TMEM119+) and astrocytes (CD45-ACSA+) were examined along with their respective sub-groups. The sample supernatants were batch analysed for inflammatory …
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Untersuchung humaner Stammzellen aus dem adulten Knochenmark und der fetalen Leber
… der in vivo Phänotyp von MSC bezüglich CD34 und CD45 analysiert. Knochenmarkzellen wurden durch FACS in die Subpopulationen CD34+, CD34-, CD45+CD34+, CD45high+CD34-, CD45med,low+CD34+ und CD45-CD34- aufgetrennt. Zellen aller Fraktionen wurden anschließend unter gleichen Bedingungen kultiviert und …
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Cellular Immune Changes in the Peripheral Blood of Subjects Chronically Infected with XMRV
… significant difference in total cellularity and CD45+ leukocytes between XMRV-infected CFS patients and XMRV negative controls, we found significant reductions in the percentage of CD45+ lymphocytes, CD45+ CD3- lymphocytes, CD45+ CD3- CD19- CD56+ NK cells and CD19+ B cells. Moreover, the NK …
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Arming adoptively transferred T-cells with drug-loaded nanoparticles for cancer immunotherapy
… regimens. On the other hand, we identified CD45 as a non-internalizing receptor on T-cells that could be used as an anchor to block internalization of cell surface-conjugated nanoparticles. Anti-CD45 decorated nanogels consisting of IL-15 superagonists remained on T-cells surfaces for over …
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Engineering cytokine immunotherapies via cell surface targeting
… targeting the ubiquitous leukocyte receptor CD45. First, we engineer and profile an αCD45-IL15 fusion that exhibits significantly diminished receptor-mediated internalization relative to its wild-type counterpart. This extended surface half-life augments downstream pSTAT5 induction and …
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Immunhistologische Charakterisierung experimenteller Lymphangiome der Maus
… experimentelle Lymphangiome induziert. Mittels CD45, einem immunhistologischen Marker für Leukozyten, konnte nachgeweisen werden, dass das Tumorgewebe in erster Linie aus einer massiven Ansammlung von Leukozyten bestand. Die Bezeichnung der durch FIA verursachten Läsion als experimentelles …
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Skeletal muscle pericyte response to acute and chronic electrical stimulation
… by multiplex flow cytometry in all samples. NG2+CD45-CD31- and CD146+CD45-CD31- pericytes were isolated following the acute study and gene expression was evaluated using high throughput qPCR. RESULTS: Acute electrical stimulation resulted in a non-significant trend for an increase in total …
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Effects of Diethylstilbestrol on Murine Early Embryonic Stem Cell Differentiation Using an Embryoid Body Culture System
… Hypothesis: Early ES cell expression of CD45 a marker common to lymphoid lineage hematopoietic stem cells and differentiation of lymphoid lineage precursors are affected by in-vitro exposure to DES. Methods: Murine ES cells were cultured using a variety of techniques: an OP9 co-culture …
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Lymph node and peri-lymph node stroma : phenotype and interaction with T-cells
… of intra-nodal and extra-nodal lymph node (CD45-) stromal cells through flow cytometry and qPCR provides a general phenotypic profile of the distinct populations. My research focused on the EpCAM+ epithelial cell population located in the fat pad surrounding the skin draining lymph nodes. …
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Characterization and Development of Strategies for Altering Protein Expression in JSL1 Cells
… its function. The T cell tyrosine phosphatase CD45 provides a valuable model for investigating the factors involved in regulating alternative splicing. The CD45 gene contains three variable exons whose splicing is regulated in response to T cell activation. Studies of this gene have revealed …
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Perivascular cell contribution to skeletal muscle repair following physiological injury
… mesenchymal stem cells (MSCs; Sca-1+CD45-) accelerate Pax7+ satellite cell expansion and fiber repair during the initial stages of eccentric exercise training in mice. MSCs are a heterogeneous population of perivascular stromal cells, including but not limited to, pericytes, …
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Modelling Embryonic Haematopoiesis Using In Vitro State-of-art Gastruloid Culture
… in flow cytometry assays (Flk-1, c-Kit, CD41 and CD45). Concomitant use of cytokines VEGF and FGF2 can facilitate the emergence of cells with the property of haemogenic endothelial cells, pro-HSCs and type 1 pre-HSCs from 96 to 144 hr. Applying Shh, Flt-3l, TPO, and SCF accordingly can promote the …
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Dietary regulation of myelopoiesis and stromal cell isolation
… stromal cell dissociation. Yield of bone marrow CD45-/Ter119-/CD31+/CD202b+ endothelial cells (ECs) and CD45-/Ter119-/CD44-/PDGFR+mesenchymal stromal cells (MSCs) were determined by flow cytometry. Liberase DL, Collagenase D, and Dispase II (all supplemented with DNase) enhanced EC and MSC …
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Molecular Mechanisms of Promyelocyte Maturation: Implications for Immune Dysfunction in Type II Diabetes
… that the removal of the tyrosine phosphatase CD45 enhances activation of protein kinase C delta (PKCdelta), and two, PKCdelta activity in conjunction with STAT6 regulate the expression of the co-stimulatory marker B7.2 (CD86) and three, the expression of CD86 and the production of cytokines in …
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Sex as a Biological Variable in Preclinical Animal Studies with an Infectious Disease Exemplar.
… revealed stable peripheral populations (CD45, CD4, CD8, CD56, CD123), with a hormone-dependent increase in CD45 expression associated with elevated estradiol levels. Vaginal microbiota composition remained stable across individuals, with increases in Mobiluncus spp. observed only during …
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Context-Dependent Function of the Splicing Factor hnRNP L
… of this mode of regulation, is the human CD45 gene. HnRNP L binds to a motif present in both CD45 variable exons 4 and 5 to affect their coordinate repression. Previously, it was shown that hnRNP L regulates exon 4 by stalling the U1 and U2 snRNPs in a non-permissive A-like exon-defined …
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