Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"CD34+ cells"”.
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Autologous Stem Cell Transplant: Factors Predicting the Yield of CD34+ Cells
… for the survival for many cancer patients. The CD34+ cell content of a collection of stem cells has appeared as the most reliable indicator of the quantity of desired cells in a peripheral blood stem cell harvest and is used as a surrogate measure of the sample quality. Factors predicting the …
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Expression and function of drug transporters in primitive CML cells
… and selectively induces apoptosis of BCR-ABL cells. However, despite excellent cytogenetic responses, only a minority of patients achieve complete molecular response (CMR). We have previously identified a population of quiescent (q) Ph+ SC found in chronic phase (CP) CML that are relatively …
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Characterization of Thy-1 expression on human hematopoietic cells
Hematopoietic stem cells have extensive proliferative capacity and the ability to produce daughter cells of all hematopoietic lineages. However, their characterization is complicated by their low frequency and the heterogeneous cellular composition of the tissues in which they are found. The …
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Mobilisation, Isolation and Coculture of Haematopoietic Stem Cells
… Autologous and allogeneic hematopoietic stem cells (HSCs) mobilized into the peripheral blood (PB) have been used as a preferred source of transplantable stem cells1-3. And umbilical cord blood (UCB) has been introduced as a more attractive HSC source for HSCT, because fetal stem cells in UCB …
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Isolation and characterization of primitive hematopoietic cells
Side-population (SP)cells are selected based on the rapid efflux of the fluorescent DNA-binding dye Hoechst 33342. Murine BM SP cells had long-term repopulating activity and proved negative for the antigen CD34. Such SP cells were detected in rats, pigs, monkeys and human cell sources. Within SP …
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The Roles of Krüppel-Like Factor 1 (KLF1) in the Human Fetal Erythroid Compartment.
… using KLF1 knockdown in in vitro-differentiated CD34+ human umbilical cord blood cells. -globin expression appears dependent on KLF1; it is increased with modest KLF1 knockdown but not in cells with low KLF1. KLF2 mRNA amounts are usually increased in KLF1 knockdown. KLF1 knockdown in CD34+ …
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Understanding Ontogeny and Optimizing Strategies to Promote Hematopoietic Engraftment Following In Utero Transplantation
… low levels of engraftment of hematopoietic stem cells (HSC) following IUHSCTx have precluded it from being curative for other diseases in which there is no selective advantage for the transplanted donor HSC. The goal of the projects and experiments presented in this dissertation was to build new …
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Proteasome inhibition in chronic myeloid leukaemia
… is antiproliferative and induces apoptosis in CD34+ cells taken at diagnosis from patients with CP CML cells, with an LD50 below concentrations achieved in vivo. We also demonstrate for the first time that CD34+38- CML cells, representing the TKI-insensitive primitive HSC, are similarly …
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Effects of bFGF (Basic Fibroblast growth factor) on the Haematopoietic Sequelae that follow transplantation
… in the clonogenic potential of stroma-adherent CD34⁺ progenitors. The bone marrow stroma is also affected, resulting in poor support for the growth of stroma-adherent multipotent progenitors that form blastic colonies (CFU-bl). To determine the possible mechanisms of marrow damage in patients …
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Preclinical evaluation of a novel telomerase inhibitor in malignant cells of the hematopoietic system
… with unlimited proliferation in most cancer cells. Recently, a non-nucleosidic small molecule inhibitor, BIBR1532, has been identified which is highly selective for the inhibition of telomerase resulting in telomere dysfunction and delayed growth arrest in tumor cells. Here we examined the …
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Novel insights into megakaryopoiesis, thrombopoiesis and acute coronary thrombosis: transcriptome profiling of the haematopoietic stem cell, megakaryocyte and platelet
… the transcriptome of human haematopoietic stem cells (HSCs), megakaryocytes and platelets to gain insights into steady state and accelerated thrombopoiesis that occurs in states of haemostatic demand and in thrombosis by applying these findings to the pathological setting of acute coronary …
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Inhibition of Mitochondrial Translation as a Therapeutic Strategy for Acute Myeloid Leukemia
… (TIG). Methods: Primary AML mononuclear cells were isolated by Ficoll centrifugation from peripheral blood. Flow cytometry dye; JC-1, Carboxy-H2DCFDA, Mitotracker GreenFM. Leukemia stem cell activity was assayed by human AML engraftment in NOD/SCID mice. Results: TIG induced cell death in …
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Development of a Human Immune System from Hematopoietic Stem Cells in a Human/Mouse Xenogeneic Model
T cells play a central role in the development of immune responses. Patients lacking T cells due to genetic defects such as DiGeorge or Nezelof's syndrome and individuals infected with the human immunodeficiency virus are highly susceptible to infections and cancers. The lack of adequate in vivo …
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Genetic and Epigenetic Interactions in in vivo and in vitro Reprogramming
… is acquisition of self-renewal. In hematopoietic cells, HOX genes are partially responsible for self-renewal, and HOX gene dysregulation commonly occurs in acute myeloid leukemia: AML). HOX dysregulation is seen in AML with translocations involving HOX genes themselves: e.g. NUP98-HOXA9) and with …
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Targeting the hematopoietic stem cell to correct osteopetrosis
… present in IMO affects the hematopoietic stem cells and hematopoiesis in general. IMO is a rare congenital disorder associated with an increased number of non-functional osteoclasts and the absence of bone resorption results in accumulation of sclerotic bone leading to abnormal bone marrow …
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The Impact of Clinically Relevant Culture on Human Haematopoietic Stem Cells: A Kinetic Analysis at Single Cell Resolution
… targeting of long-term haematopoietic stem cells (LT-HSCs), which are characterised by a predominantly quiescent status and sustained self-renewal capacity. Clinical ex vivo gene therapy protocols target the heterogenous mix of stem and pro-genitor cells encompassed in the CD34+ fraction …
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New approaches to improve the cord blood unit hematopoietic progenitor cell content
… of cord blood unit hematopoietic progenitors (CD34+ cells and colony-forming units); this is a novel finding. Mean platelet volume can be thought to represent general hematopoietic activity, as newly formed platelets have been reported to be large. Stress during delivery is hypothesized to lead …
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Separation and Further Characterization of Hematopoietic Cell Populations Based on Phenotypic and Biophysical Properties
Hematopoietic stem cells (HSC) are multipotent and possess self-renewal capacity. Thus, they can sustain a life-long hematopoiesis and are also capable of restoring the hematopoietic system when transplanted to a patient. In allogeneic hematopoietic stem cell transplantation (HSCT), a potent …
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Single-cell Protein-Transcriptome Atlas of Haematopoiesis across the Human Lifespan
… the continuous production of stem and progenitor cells, which divide and mature to produce a wide variety of specialised circulating blood cells. Proper regulation of lineage decisions is key to ensure the right balance between the generation of differentiated cells and the maintenance of the …
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Establishing models of and characterising the genomic context of neutrophil hypersegmentation
… highlight that neutrophils are multifaceted cells with an active transcriptional program. Further, this work reveals that neutrophil hypersegmentation is not a homogenous phenomenon, but rather a phenotype that occurs in response to multiple stimuli via distinct mechanisms. The thesis also …