Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 80 for “"CD14"”.
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The Role of the CD14 molecule in equine endotoxemia
Objectives - To evaluate the effects of equine sCD14 and monoclonal antibodies (mAbs) to equine CD14 on LPS-induced TNF° expression of equine peripheral blood mononuclear cells (PBMCs). To determine serum concentrations of soluble (sCD14) in a population of horses with gastrointestinal diseases or …
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Untersuchungen zur Assoziation genetischer Polymorphismen im Gen des Endotoxinrezeptors CD14 mit der transkriptionellen Aktivität
Assoziationen des SNPs rs2569190 im CD14-Endotoxinrezeptorgen mit dem Risiko für verschiedene Erkrankungen, die mit einer Entzündungskomponente einhergehen, und weitere in-vitro-Experimente mit transfizierten Zelllinien lassen vermuten, dass das T-Allel der polymorphen Position über eine erhöhte …
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Mapping the immunological receptors CD14 and macrophage receptor with collagenous structure (MARCO) and their innate recognition potential on trophoblast cells: relevance for human pregnancy
… and plays a valuable role in association with CD14. The interaction of CD14 and MARCO was explored with the use of confocal microscopy which showed physical associations, most likely contributing to their function at the feto-maternal interface. Trophoblast responses to LPS indicated a …
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Bedeutung des löslichen CD14-Rezeptors in Plasma und Urin als immunologischer Parameter nach Nierentransplantation und sein Verhältnis zu den löslichen Rezeptoren IL2R, CD4 und CD8
Der lösliche CD14 (sCD14) kann in erhöhten Konzentrationen im Plasma und Urin bei einer Aktivierung des Immunsystems gemessen werden. Für Nierentransplantierte ist die Vermeidung oder frühzeitige Detektion und Behandlung von Komplikationen von wichtiger prognostischer Bedeutung. Diese Arbeit …
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Monozyten-Populationen und ihre Zytokin-Produktion beim Erysipel
… unterschieden werden, die „klassischen“ CD14++CD16-DR+ Monozyten und die proinflammatorischen CD14+CD16+DR++ Zellen. Letztere machen nur ungefähr zehn Prozent aller Monozyten aus und sind bei zahlreichen Erkrankungen im zentralen Blutpool stärker vermehrt als die klassischen Monozyten. …
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Structural and ligand binding studies of toll-like receptor 4 (TLR4) associated proteins
… function of soluble or membrane anchored human CD14 is to bind and shuttle LPS to TLR4/MD-2, thereby enhancing host sensitivity of LPS. The mechanism by which CD14 binds and transfers LPS remains unknown, and our understanding of this process would be advanced by structural information. Thus, we …
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Ex Vivo Induction of Human Regulatory T Cells
… receptor stimulation of UCB T cells via a CD14+ monocyte population leads to a predominant induction of Tregs.</p><p>Further studies identified a CD14+ cell subset with increased cell surface expression of CD36 and the TGF-beta related latency associated peptide, LAP. We termed this unique …
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The role of Poly (ADP-ribose) polymerase in the regulation of innate recognition of immunological receptors expressed on human macrophages.
… system uses a variety of receptors including CD14, TLR4, TLR2 and SR (MARCO). These receptors are expressed on most human monocytes and macrophages. CD14 recognizes and bind lipopolysaccharide (LPS), the main causative agents of sepsis and endotoxic shock. Signaling from CD14-LPS and TLR4/MD-2 …
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Monozytäre Expression von CD 14 ("Endotoxin-Rezeptor") und proinflammatorische Subpopulationen antigenpräsentierender Leukozyten
… cell sorter (FACS))gemessene Expression sog. mCD14-Rezeptoren und der CD14+CD16++-Rezeptoren auf peripheren Blut-Monozyten mit serologischen Parametern wie C-reaktives Protein und Leukozytenzahl. Die Frage, ob die beiden Oberflächenantigene CD14 und CD16 sowie die Koexpression beider Antigene …
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Unravelling the anti-inflammatory mechanisms of dietary fatty acids
… expression of TLR4 and its associated molecule CD14 in response to LPS, but had no effect on LPS binding to TLR4. The exact mechanism of the effects of PUFA on CD14 was elucidated by examining lipid raft 'microdomains', the location where the receptor complex clusters upon activation. We found …
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The recognition of lipopolysaccharides present in Gram-negative bacteria by receptors of the immune system
… the cluster of differentiation receptor 14 (CD14). CD14 functions with the co-receptor known as Toll-like receptor 4 (TLR-4), which in the presence of an accessory receptor known as myeloid differentiation 2 (MD-2) triggers immunity against gram-negative bacteria. Experiments were designed to …
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Probiotic Modulation of Mucosal Immune Responses in an In Vitro Co-Culture Model
… resemble the M2 subset and fail to express CD14, a co-receptor for LPS signalling. Therefore, probiotic modulation of LPS-induced NF-kB activity and cytokine expression was investigated using a THP-1 monocyte-derived reporter cell line, model of CD14 hi / lo M1 and M2 macrophages. Secondly, …
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Intestinal Inflammatory Responses to the Indigenous Microbiota
… alone. The ability of TNF-alpha-to upregulate CD14 and Toll-like receptor (TLR) expression and thereby enhance IL-8 mRNA expression in response to L. plantarum 299v was investigated. Baseline expression of CD14 mRNA and cell-surface CD14 was not altered by TNF-alpha. HT-29 cells did not express …
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The role of HLA-G in bone marrow transplantation
… of graft-versus-host disease. We identified a CD14+ HLA-Gpos population with an HLA-DRlow phenotype and decreased in vitro immunostimulatory capacity circulating in peripheral blood of healthy individuals. Naturally occurring CD14+HLA-Gpos cells suppressed T cell responses and acted …
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An in-vitro investigation to determine the neuroinflammatory response of CNS cells to oral bacteria and their virulence factors.
… antibodies to LPS, and its specific receptor CD14, and gingipains. The Bioplex magnetic bead array analysis from Bio-Rad was performed to measure cytokine release by the human cell lines (IMR32 and SVGp12). In addition a second ELISA assay specifically for TNF- release was also employed with …
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Phenotypic characterization of stress Leukocytosis
… that of the three major monocyte populations; CD14++CD16, C14++CD16+ and CD14+CD16+, the proinflammatory‟ CD14+CD16+ monocytes showed the largest mobilization response during stress and isoproterenol infusion. Thus, the selective mobilization of cells with a high effector ability applies to …
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Relative level of glucocorticoid and progesterone receptor: implications for immune and reproductive functions in women
… CD3+, CD4+, and CD8+ T lymphocytes, as well as CD14+ monocytes, express higher levels of GR than PR. Models and methodologies: PR-negative COS1 cells overexpressing exogenous GR or PR proteins were used to assess cross-reactivity of primary antibodies by western blot analysis, immunofluorescence …
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Expression and Function of Toll-Like Receptor 2 and 4 in Human Melanocytes
… of TLR2, TLR4 and their adaptor proteins, CD14 and myeloid differentiation factor-88 (MyD88) was shown by RT-PCR. Protein expression and localization of TLR2, TLR4 and CD14 were detected by western blot, flow cytometry and immunocytochemistry. Lipopolysaccharide (LPS) was used as microbial …
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Proteomic analysis of TRIF interactome and functional characterisation of novel TRIF interacting proteins
… cytokine/chemokine secretion in the U373-CD14 cell line. This study also demonstrated that all of the three DVLs isoforms (DVL1, DVL2 and DVL3) interacted constitutively with TRIF when overexpressed in HEK293-TLR4. However, immunoprecipitation of endogenous TRIF in U373-CD14 cell line …
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DESIGNING BIOMIMETIC NANOPARTICLES TO TACKLE INTRACELLULAR PATHOGENS
… of human monocytes (THP-1 cells and primary CD14+ monocytes). These NGs were designed to retain key membrane features while excluding nuclear and cytoplasmic content, with the goal of targeting intracellular Mab more effectively. We adopted a stepwise experimental strategy using orthogonal …
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