Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 33 for “"CD spectroscopy"”.
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Structural Investigations of Nematode Anhydrobiotic and Rab11-FIP Proteins by Far-UV Circular Dichroism and Mid-IR Fourier Transform Infra-Red Spectroscopy
… hemoglobin, lysozyme and a-chymotrypsin by CD spectroscopy resulted in excellent agreement of estimated fractional composition with that observed by x-ray crystallography. CD spectroscopy was found to be very accurate for estimating a-helix and random coil content, but considerably less so …
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Probing peptide binding to the second PDZ domain of hPTP1E using FT-IR and MS
… three peptides was investigated using FT-IR and CD spectroscopy. The T m of free PDZ in D 2 O was found to be 47 ± 1C̕ when monitoring the loss of Ý-sheets and the appearance of aggregation by FT-IR. Combinations of PDZ with KDDEVYYV, ENEQVCAV and ENEQVSAV revealed T m values of 48 ± 1, 49 ± 1 …
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CHARACTERIZATION OF THE RNA BINDING MODE OF A PLATINUM-ACRIDINE AGENT
… were also analyzed by circular dichroism (CD) spectroscopy.
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Synthesis and applications of oligonucleotide-bound alginate hydrogels
… investigates the use of circular dichroism (CD) spectroscopy for label-free detection of DNA and RNA hybridization in oligonucleotide-bound alginate hydrogels, with applications in biosensing. Incorporating 2’-fluoro modifications significantly enhanced sensitivity, with 2’-F probes …
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Studies on Anthracene Tagged Oligonucleotides
… UV melting point studies, fluorescence and CD spectroscopy in order to understand the effect of varying base pairs and introducing mismatches. Certain duplexes were then studied in greater depth using transient absorption spectroscopy, to probe the formation of electron transfer …
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Branching into RNAi: Synthesis, Characterization and Biology of Branch and Hyperbranch siRNAs
… hyperbranch motifs were then studied by CD spectroscopy and thermal denaturation experiments. CD spectroscopy was used to characterize the requisite A-type double-stranded RNA helix for RNAi application; whereas thermal denaturation experiments were used to validate siRNA hybrid …
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Structure-Function Studies of a Truncated Ice-Nucleation Protein
… P141-A220 and the other encoding G1021-E1196. CD spectroscopy of this recombinant protein indicated it had β-rich secondary structure that was increased by detergent. The construct shaped ice into a hexagonal bipyramid at high concentrations, but evaluation of antifreeze activity was …
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Identification of Critical Residues in Spliceosomal Protein Dib1
… mutational analysis, protein purification, and CD spectroscopy were performed.
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Copper-catalyzed arylation of 1,2-amino alcohols. Synthesis of N-terminal, peptide helix initiators, and characterization of highly helical, capped polyalanine peptides
… NH to ND exchange in D₂0, observed by NMR; and CD analysis. CD spectra of these helical peptides were used to directly verify an equation used for peptide structural analysis. The helix-initiating N-terminal cap (Hel) used to create the highly helical polyalanines is a synthetically modified …
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Side chain interactions at the dimer interface of Vibrio alkaline phosphatase determine its reactivity, stability and desthiobiotin inhibition
… and three of them were used for this study. CD spectroscopy monitors a change in the enzymes circular dichroism due to changes in its secondary structure. Fluorescence spectroscopy detects a change in the environment of Trp residues that cause a shift in λmax when the protein is denatured. …
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The Influence of the Cellular Environment on Z-DNA Formation
… B-Z transition. We utilized circular dichroism (CD) spectroscopy to monitor the B-Z transition in a divalent ion background in the presence and absence of a model osmolyte, PEG 200. Our results thus far suggest that PEG 200 greatly enhances the formation of Z-DNA in the presence of Mg2+ as …
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Porphyrin-DNA as scaffold for nanoarchitecture and nanotechnology
… of techniques such as UV?vis and fluorescence spectroscopy, UV?vis and fluorescence melting studies, circular dichroism spectroscopy and EPR (electron paramagnetic resonance) spectroscopy showed electronic interaction between porphyrins stacked on DNA. Metallation with zinc or copper of …
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Stability of Nucleic Acid Secondary Structures and Their Contribution to Gene Expression
… UVabsorption and Circular Dichroism (CD) spectroscopy and Differential Scanning Calorimetry (DSC). The combinatorial analysis indicates that an energetically favorable stem-loop structure forms in all solution conditions examined. The stem-loop structure is stabilized at very high salt …
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Expression, purification and characterization of the structural properties of recombinant Pysp1 and Pysp2 spidroins
… of the purified PySp C-terminal domains using cd spectroscopy and atomic force microscopy. After expression and purification of the PySp C-tennini proteins, our studies support that this domain displays a predominantly ~-sheet structure, distinctive from the NMR-determined ahelical nature of …
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RNAi Nanotechnology: A Platform for siRNA Screening and Cancer Gene Therapy
… by native PAGE, thermal denaturation and CD spectroscopy. The data supported the stable 3CS self-assembly on a thirty nucleotide (30nt) RNA template and with complementary 15nt and 23 nt RNA sequences in Tris buffer. These conditions were shown to favor the self-assembly of higher-order …
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Cytotoxic Activity of Non-Specific Lipid Transfer Protein (nsLTP1) and Proteomic Evaluation of Heparin-Binding Proteins from Ajwain (Trachyspermum ammi) Seeds
… EGFR, and VEGF genes expression using RT-PCR. CD spectroscopy analyzed the nsLTP1 conformational changes after thermal treatment for structure stability determination. The RP-HPLC was used to analyze the nsLTP1 degradation rate in human serum at different time intervals incubated at 37°C. …
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Re-evaluation of analytical chemistry techniques in studying DNA structures
… calorimetry (DSC), ultra violet visible (UV-Vis) spectroscopy, circular dichroism (CD) spectroscopy, and high-performance liquid chromatography (HPLC). 1) d(CG)9 and a mixed sequence in the B- and Z-DNA conformation was examined by CD and UV-Vis at a concentration of 1mM using a home-made cuvette …
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Hexagonally-Packed Helical Oligo(m-Phenylene Ethynylene) Nanotubules via Structural Modification and Guest Templation
… in solution by UV-vis, fluorescence, and 1H NMR spectroscopy, as well as in the solid state by wide- and small-angle X-ray powder diffraction (WARD and SAXD). Terpene guest binding was also examined using circular dichroism (CD) spectroscopy. The addition of endo-phenoxy methylene tert-butyl …
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Synthesis, Characterization and Device Studies of Conjugated Foldamers
… studied to shed light on their folding process. CD spectroscopy was used to confirm the presence of helical folding present in the polymers. The device properties of INDT-based polymers were found to be 0.026 and 0.18% for polymers 20 and 22, respectively. In the last chapter, we have synthesized …
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The Structural Characterization and Bioactivity Assessment of Nonspecific Lipid Transfer Protein 1 (nsLTP1) from Caraway (Carum Carvi) Seeds
… of nsLTP1 was examined by circular dichroism (CD) spectroscopy.</p> <p><strong>Results</strong>: Caraway nsLTP1 is composed of 91 residues and weighs 9652 Da. The three-dimensional structure of caraway nsLTP1 sequence was constructed based on searching known structures in the PDB. We chose …
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