Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"CAZymes"”.
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Functional metagenomic discovery and characterisation of CAZymes by microfluidic methods
… genes coding for carbohydrate-active enzymes (CAZymes) are very often organised in clusters and the corresponding enzymes act together in the concerted degradation of large carbohydrate substrates. Therefore, it would be more desirable to screen metagenomic libraries with larger inserts …
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Marine carbohydrate-active enzymes illuminate microbial ecology, evolution, and carbonate precipitation
Marine carbohydrate-active enzymes (CAZymes) in microbial mats build, modify, and degrade the matrix of exopolymeric substances (EPS) that structure these ancient ecosystems and control mineral formation via the alkalinity engine and organic surfaces involved in mineral nucleation and growth. …
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Design characterisation and in planta expression of a chimeric xylanase
… processes. Carbohydrate Active enZymes (CAZymes) may reduce recalcitrance, through heterologous expression directly in the lignocellulosic biomass. CAZymes from extremely thermophilic organisms are not normally active at the mesophilic temperatures, allowing for accumulation in the …
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The role of interfacial and 'entropic' enzymes in transitory starch degradation : a mathematical modeling approach
… perspective on carbohydrate-active enzymes (CAZymes) but is motivated by the organization of the downstream pathway of starch breakdown. This comprises Alpha-1,4-glucanotransferases (DPE1 and DPE2) and Alpha-glucan-phosphorylases (Pho or PHS) both in the stroma and in the cytosol. CAZymes …
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Modeling Nitrogen and Energy Metabolism in the Bovine
… of carbohydrate-active enzyme transcripts (CAZymes), fiber degradation, and short chain fatty acid (SCFA) concentrations. To achieve the first objective, a total of 229 studies (n = 938 treatments) including dairy and beef cattle data, published from 1972 through 2016, were collected from …
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Harnessing Deep Learning with Protein Language Models to Unveil Microbial Enzyme Function in Health and Disease
… the annotation of Carbohydrate Active Enzymes (CAZymes) in metagenomic datasets. I applied CAZyLingua to a paired mother/infant longitudinal dataset and revealed unannotated CAZymes linked to microbiome development during infancy. When applied to metagenomic datasets derived from patients …
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Genomic Evolution in the Oomycetes
… protein level. Comparative genomic analysis of CAZymes suggest that oomycete lifestyles may be linked to their CAZyme repertoires. Overall, the data presented in this thesis expands our knowledge of oomycete genome evolution.
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Advancing detection, genomics, and secretome analysis of oomycete pathogens Phytophthora capsici and Phytopythium vexans to explore mechanisms of pathogenesis and biocontrol solutions
… genomes, additionally SS21 displayed a reduce CAZymes, RxLR and CRN effectors, suggesting a niche adaptation to a specific Canadian agro-ecosystem. The final component of this thesis focuses on the development of an amplitude-based multiplex droplet digital PCR (ddPCR) assay. Designed for …
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Community Composition and Function of the Hindgut Microbiome in Seaweed-Eating Marine Fish
… endo-acting carbohydrate-active enzymes (CAZymes) associated with both classes suggest their involvement in initiating glycan depolymerization. Bacteroidia contributed the most to CAZyme-related gene expression in the hindgut, particularly in the biomass-rich hindgut chamber. The …
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Identification and characterisation of lignin degrading bacteria and enzymes fromlarval guts of the African Palm Weevil (Rhynchophorus phoenicis)
… bacterial metagenome aligned with genes encoding CAZymes and 249 of these belonged to the “Auxiliary Activities” class which harbours the suite of genes implicated to play different roles in lignin deconstruction. Three genes of putative lignin degraders were successfully amplified by PCR, one of …
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Polysaccharide utilization by the human colonic bacterium, bacteroides intestinalis DSM 17393
… arrays of carbohydrate-associated enzymes (CAZymes) responsible for degrading polysaccharides. Furthermore, these bacteria have highly organized gene clusters, known as polysaccharide utilization loci (PUL), encoding the enzymes required for the degradation and transport of the sugar …
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Bacteria Big and Small: Exploring the Gut Microbiota of Marine Herbivorous Fish and Student Learning About Cell Size
The gut microbiota of animals include a diverse array of bacteria of different shapes and sizes ranging from about 1 to 600 μm in length. These microbes perform important functions such as influencing the host circadian rhythm and breaking down dietary polysaccharides. The first chapter of this …