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Showing 1 to 6 of 6 for “"CARS Microscopy"”.
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Imaging biological water permeability barriers using CARS microscopy
… Coherent anti-Stokes Raman scattering (CARS) microscopy, offers a means to visualize the water permeability barrier of biological structures at high resolution within intact systems. In this thesis, water CARS imaging was characterized for determining water permeability in both dynamic …
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Femtosecond CARS Microscopy to characterize lipid droplets in Engineered Adipose Tissue
… microscopic techniques, such as fluorescence microscopy, provides excellent selectivity of lipid-based structures inside adipose tissue cellular structures based on staining with compound dyes. However, fluorescence staining limits multiplex imaging, and requires time consuming steps in …
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Developing Single-Laser Sources for Multimodal Coherent Anti-Stokes Raman Scattering Microscopy
Coherent anti-Stokes Raman scattering (CARS) microscopy has developed rapidly and is opening the door to new types of experiments. This work describes the development of new laser sources for CARS microscopy and their use for different applications. It is specifically focused on multimodal …
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Nonlinear interferometric vibrational imaging for applications in the study and diagnosis of breast cancer
… thesis deals with the problem of developing a microscopy imaging technique with fast acquisition rates and label-free chemical selectivity for the study of tumor formation and its diagnosis in early stages of development. Spontaneous Raman scattering microscopy studies on human breast …
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Oh cryoprotectant, wherefore art thou cryoprotectant? Investigation of the permeation of common cryoprotectants into live rice callus cells by coherent Raman microscopy
… Vibrational microscopies, like Raman or infrared microscopy, allow the direct visualization of cryoprotectants interacting with living cells. By exciting vibrations unique to the bonds in a molecule, these techniques can effectively image nearly unadulterated molecules. Through deuteration, …
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Polarization Controlled Coherent Anti-Stokes Raman Scattering Microscopy for Determination of Structural Order of the Myelin Sheath
Coherent Anti-Stokes Raman Scattering (CARS) is a laser-scanning microscopy technique that generates a strong label-free signal in lipid. The myelin sheath surrounding nerves is up to 80% lipid, and therefore is an ideal candidate for CARS imaging. The long lipid chains forming the myelin wraps …