Global ETD Search
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Showing 1 to 6 of 6 for “"CAF-1"”.
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Investigation of The Roles of Asf1 and Caf-1-Mediated Chromatin Assembly In The Human Dna Damage Response
… via knockdown of the histone chaperones ASF1A, CAF-1 or a mutation that specifically prevents ASF1 binding to histones, hinders loading of Rad51 onto ssDNA during homologous recombination, as a consequence of reduced recruitment of the Rad51 loader MMS22L/TONSL to ssDNA, resulting in persistent …
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Expression, purification and characterisation of recombinant chromatin assembly factor 1
Chromatin Assembly Factor 1 (CAF-1) is the only known replication dependant histone chaperone, responsible for the deposition of the histone H3/H4 tetramer onto DNA. Found in all eukaryotes, CAF-1 consists of three subunits, p150, p60 and p48. Since its identification work on CAF-1 has mainly …
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Development of a TIRF Microscope for Single Molecule Binding Studies
… study the crucial interaction between PCNA and CAF-1 in replication-coupled nucleosome assembly to contribute to epigenetic inheritance. The microscope measures fluorescence following total internal reflection (TIR) to accomplish single molecule imaging (SMI). TIR provides a high signal-to-noise …
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Chromatin and transcriptional regulators act in a cascade to establish a bilateral asymmetry of the C. elegans nervous system
… DNA by the heterotrimeric protein complex CAF-1. We observed that loss of function of each of three genes encoding members of the C. elegans CAF-1 complex transformed MI into an e3D-like cell. We propose that CAF-1-mediated nucleosome formation is impaired by the presence of mutant HIS-9 …
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Characterizing the Role of SUMO in Transcriptional Repression in S. cerevisiae
… that Rlf2, a component of the histone chaperone CAF-1, is able to bind SUMO in S. cerevisiae. This raises the possibility that CAF-1 plays a role in SUMO-mediated transcriptional repression. Secondly, I used ATAC-Seq to demonstrate that SUMO system inhibition may increase chromatin openness, …
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The structural analysis of histone H3 lysine 56 acetylation and related histone chaperone complexes
… chaperone complex Chromatin Assembly Factor 1 (CAF-1) is responsible for depositing H3.1/H4 tetramers in a DNA synthesis dependent manner. During replication independent assembly H3.3/H4 is deposited by Histone Regulator (Hir) complex. In both instances nascent histone H3/H4 dimers are supplied …