Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"C57BL/6 mouse"”.
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In silico prediction of C57BL/6 mouse T-cell epitopes: enhancing immunogenicity assessment with PREDBL6
… system for identifying T-cell epitopes in C57BL mice by integrating relevant contributing factors, such as the antigen processing steps before MHC binding and thermostability, with the MHC binding predictions. Utilizing deep learning methods, we first trained and rigorously validated the …
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Acute Effects of Whole-Body Proton Irradiation on the Immune System of the C57BL/6 Mouse
… from a 60cobalt (60Co) source. Adult, female C57BL/6 mice were exposed to a single dose (3 Gy, 0.4 Gy/min dose rate) of either proton WBI at the Bragg peak, proton WBI at the entry plateau, or Co WBI, and sacrifice intervals were at 1,4, 7, and 10 days post- WBI. Flow cytometry analysis of the …
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Synthetic and Natural Environmental Compounds as Potential Facilitators of Mptp-Induced Parkinsonism
… motor deficits similar to PD. MPTP used in the C57BL/6 mouse is a widely used animal model of PD. The pyrethroid permethrin (PM), and the organophosphate chlorpyrifos (CPF), can produce changes in dopaminergic nigrostriatal neurons, the primary target of PD and MPTP-induced neurotoxicity. Such …
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Transgenic complementation of rumpshaker with wild type proteolipid protein
… A single base change mutation in our spontaneous mouse model rumpshaker (Plpjp-rsh)(Ile186Thr) (Schneider et al., 1992) generates a misfolded protein resulting in dysmyelination and increased numbers of apoptotic oligodendrocytes. The phenotype varies from mild on the original C3H background to …
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The Effect of Primary Hepatocyte Expressing HBx on the Activation and Apoptosis of Antigen-specific CD8+ T Cell
… naive CD8^(+) T cells were cultured with C57BL/6 mouse primary hepatocytes infected with baculovirus expressing HBx. At the end of co-culture, cell proliferation, cytokine production and apoptosis of CD8^(+) T cells were analyzed. As the results, the cell proliferation of naive CD8^(+) T …
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Severe Acute Respiratory Syndrome Coronavirus-2 Vaccine Development: A Virus-Like Particle Vaccine Approach
… was tested utilizing a K18-hACE2 transgenic C57BL/6 mouse model. Mice were challenged with live SARS-CoV-2 three weeks after the final booster dose and the vaccine was evaluated for protective efficacy. Results of these studies showed epitope-specific humoral and cell-mediated immune …
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TARGETING CANCER METABOLISM WITH FASTING-MIMICKING DIET¿BASED LOW-TOXIC COMBINATION THERAPIES: OVERCOMING RESISTANCE THROUGH METABOLIC SYNERGY
… both immunodeficient (NSG) and immunocompetent (C57BL/6) mouse models, without inducing major toxicity. However, as previously demonstrated in our studies, cancer cells resist FMD through upregulation of starvation escape pathways. Here, mechanistically, RNA sequencing (RNA-seq) analysis revealed …
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The Role of the Complement System in Mousepox Infection
… regulatory protein. Ectromelia virus provides a mouse model for human smallpox infection where the virus and the host's immune response have also co-evolved. Using this model, we investigated the role of the complement system in a poxvirus infection. Ectromelia virus inoculated via multiple …
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Potential Mechanisms Explaining the Antitumor Effect of Total-Body Irradiation
… Gray of γ-rays) preceded EEC implantation in the C57BL/6 mouse model. Tumor volumes and mouse weights were measured throughout each protocol; and immunohistochemical analyses were performed on tumors excised from control and test mice to evaluate leukocyte infiltration. In later studies, mice were …
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Investigating the protective effects of intestinal GABA[subscript A] receptor activation on an animal model of Multiple Sclerosis
… control strain (pAC-L. lactis). EAE-induced C57BL/6 mice treated with P8 GAD-L. lactis (n=10) had significantly lower clinical scores than the sham control group (autoclaved media; n=10) and the L. lactis control group (pAC-L.lactis; n=10). Despite trying, we were unable to repeat this …
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Developmental Exposure to 2,3,7,8-Tetrachlorodibenzo-p-dioxin: Induced and Exacerbated Autoimmunity in Adulthood
… high affinity aryl hydrocarbon receptor (AhR) C57BL/6 mice resulted in an immune complex-mediated autoimmune disease during the adult stage. Further using a similar TCDD exposure protocol, autoimmune-prone low affinity AhR SNF1 mice exhibited acceleration and exacerbation of lupus-like …
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Host response to Rift Valley fever virus infection
… In the first part of my project, primary mouse bone marrow derived (BMD) macrophages were infected with MP-12 (attenuated vaccine strain), rMP-12 Clone13 type virus (a recombinant virus resembling a naturally occurring attenuated strain), and ZH501 (pathogenic wild type strain). The …
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Immunotoxicity of Pesticide Mixtures and the Role of Oxidative Stress
… of multiple pesticide exposure were evaluated. C57BL/6 mouse thymocytes were exposed to lindane, malathion, and permethrin, either separately or in mixtures of two pesticides, in concentrations ranging from 37.5 uM to 1mM. These exposures caused both apoptotic and necrotic cell death in …
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GENERATION OF SYNTHETIC ANTIBODIES AS POTENTIAL THERAPEUTICS TO BLOCK IL3–MEDIATED INNATE IMATINIB RESISTANCE IN CHRONIC MEYLOID LEUKEMIA
… antibodies showed a prolonged survival of C57BL/6 mouse models that received antibody–treated CML cells. Encouraged by anti–leukemic activity of anti–IL3Rα and anti–IL3Rβ antibodies on CML cells, we used our novel antibodies to engineer anti–IL3Rα–IL3Rα and anti–IL3Rβ–IL3Rβ bivalent and …
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Ionizing Radiation and Endostatin Gene Therapy Against Lung Tumor
… <p>pXLG-mEndo, the unique plasmid containing a mouse endostatin gene under the control of a cytomegalovirus (CMV) promoter was constructed in our laboratory. The functionality of pXLG-mEndo was tested in vitro using both human umbilical vein endothelial cell (HUVEC) and Lewis lung carcinoma …
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Changes in the Murine Nigrostriatal Pathway Following Pyrethroid and Organophosphate Insecticide Exposure: An Immunohistochemical Study
… protein (DAT), within the striatum of the C57BL/6 mouse, using DAT antibodies, following low (0.8, 1.5 & 3.0 mg/kg) and high (200 mg/kg) doses of PE, respectively. Possible nigrostriatal terminal degeneration was examined using antibodies to tyrosine hydroxylase (TH), the rate-limiting …
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Analysis of nerve terminal dysfunction in autoimmune neuropathy
… dependent, glial and/or neuronal injury at mouse neuromuscular junctions (NMJs). Three antibodies (EG1, LB1 and R24) were identified as producing selective terminal Schwann cell (TSC) injury whilst sparing neuronal membranes at NMJs in BALB/c and C57BL/6 mouse strains in a dose dependent …
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The Neurotoxicity of Insecticides to Striatal Dopaminergic Pathway
… potential relationship to Parkinsonism in the C57BL/6 mouse model, using biochemical and molecular biology methods. In the first objective, I investigated the neurotoxicity in striatal dopaminergic pathways following co-application of permethrin (PM), chlorpyrifos (CPF) and …
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Treatment of murine tumors with recombinant Myxoma viruses
… viral treatment for cancer in immunocompetent mouse models of cancer. In addition, a recombinant MYXV that expresses the murine gene for interleukin 15 (IL-15) was also used, in order to determine if increased expression of IL-15 within the tumor bed could improve MYXV treatment by recruiting …
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ROLE OF IL-10 PRODUCING EOMES+ TR1-LIKE CELLS IN MELANOMA
… antibodies. In this study, we employed the C57Bl/6 mouse model of B16-OVA melanoma to investigate the molecular mechanisms by which EOMES+ Tr1-like cells modulate anti-tumor T cell responses in vivo. Our findings reveal that EOMES+ Tr1 cells producing IL-10 are significantly enriched in …
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