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Showing 1 to 20 of 35 for “"C3a"”.
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Reactions for rapid access to C3a oxygenated pyrroloindolines and its application towards a novel route to pestalazine A
… systems the most common functionality at the C3a position is perhaps the hydroxyl group. In this work oxoammonium salt 4-acetamido-2,2,6,6-tetramethyl-1-oxopiperidinium tetrafluoroborate (Bobbitt’s salt) rapidly reacts with tryptamine and tryptophan derivatives to provide the C3a oxygenated …
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Total synthesis of cyclotryptamine and diketopiperazine alkaloids and biosynthetic pathway investigation of unsymmetrical diketopiperazine dimers
… completely stereoselective formation of all C3a-C3a' and C3a-C7' carbon-carbon bonds and all the associated quaternary stereogenic centers. The synthesis of these complex diazenes was made possible through a new methodology for synthesis of aryl-alkyl diazenes using electronically attenuated …
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Total synthesis and anticancer evaluation of all known communesin alkaloids and related complex derivatives
… two advanced fragments to secure the congested C3a-C3a' linkage in three steps, followed by a highly efficient biogenetically inspired aminal reorganization to access the heptacyclic communesin core in only two additional steps. Enantioselective syntheses of the two fragments were developed, …
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The role of anaphylatoxins in asthma and airway remodelling
C3a and C5a anaphylatoxins are proinflammatory polypeptides released during complement activation. They exert their biological functions by interacting with the G protein-coupled receptors, C3aR and C5aR respectively. Activation of the complement system has been implicated in the pathogenesis of …
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Complement activation and effector pathways in ischaemia reperfusion injury and at the interface with adaptive immunity
… been shown to play a part in the genesis of IRI, C3a could be shown to have an important effect on innate cells that form a bridge with adaptive immunity. The aim of the work described in this thesis was therefore to investigate: (a) the role of the lectin pathway in IRI through the enzyme MASP-2; …
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The regulation of allergic airway disease by type V collagen-induced tolerance
… transcripts. Bronchoalveolar lavage levels of C3a¸ C5a and OVA-specific IgE were measured; and immunohistochemistry utilized to detect expression of complement regulatory proteins, expression, CD46/Crry and CD55, in lung tissue. Results: Compared to normal subjects, anti-col(V) antibodies were …
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A study of the protective effects of an aspalathin enriched green rooibos extract on experimentally induced hepatic steatosis
… NAFLD. Methods: The study used immortalized C3A liver cells and oleic acid to induce steatosis. Assessment of the effect of GRT on oleic acid induced steatosis was determined using different concentrations of aspalathin-enriched rooibos (GRT) extract. Cells not exposed to oleic acid were …
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Total Synthesis of Oligomeric Cyclotryptamine Alkaloids
… whole cyclotryptmines to iteratively introduce C3a−C7' quaternary linkages on an advanced heterodimeric intermediate with full stereochemical control at each quaternary linkage. We developed a strategy for iterative aryl-alkyl diazene synthesis using increasingly complex oligomeric hydrazide …
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Defining the pro-tumour impact of the evolving stromal microenvironment
… by production of C3 and its cleavage product C3a. Significantly, inhibition of the C3a/C3aR axis reduced the number of macrophages and decreased tumour volume. This reduction in tumour growth was accompanied by increased CD8 T-cell infiltration, implying that ‘immune’ CAFs may inhibit adaptive …
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Investigating the role of the complement system in the pathogenesis of sub-retinal fibrosis in neovascular age related macular degeneration
… has previously shown that complement component C3a is increased in the serum of patients with sub-retinal fibrosis. Chapter 5 presents the findings that complement component C3a can mediate MMT in bone marrow derived macrophages in vitro. We found that blocking C3a during the two-stage laser …
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Production and Characterization of Monoclonal Antibodies against a Recombinant Nfa1 Protein Cloned from Pathogenic Naegleria fowleri
… RESULTS: Eight McAbs (A1F, A3C, A6D, A10F, C3A, C6F, C7F, B6B) against the rNfa1 protein were obtained. Eight McAbs isotypes were all IgG2b. The McAbs had high affinity against the recombinant His-tag fusion Nfa1 protein, which was resulted by indirect ELISA. They recognize 17 kDa protein …
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Novel Endogenous Antimicrobial Peptides
… capacity were discovered for the anaphylatoxin C3a, generated during activation of the complement system, and the inactive derivative of C3a (C3adesArg), as well as shorter synthetic peptides from the molecule. Novel antimicrobial activity was also shown for the heparin binding and cell-binding …
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THE ABSENCE OF C3AR AND C5AR SIGNAL TRANSDUCTION PROMOTES T REGULATORY CELL DIFFERENTIATION AND REGULATES IMMUNOLOGIC TOLERANCE
Here, we demonstrate that when C3aR and C5aR signals are not transduced into CD4+ T cells, PI-3K¿-AKT-mTOR signaling ceases, PKA activation increases, auto-inductive TGF-ß1 signaling initiates, and CD4+ T cells become Foxp3+ T regulatory cells (iTregs). Endogenous TGF-ß1 suppresses C3aR and C5aR …
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Changes in tear film biochemistry in end of day ocular discomfort with and without contact lenses
… protein, proline rich protein 4, resolvin D1 and C3a are reported for the first time. Concentrations of lysozyme and lactoferrin, secretory phospholipase A2, bradykinin, prostaglandins, cysteinyl leukotrienes, resolvin D1, phosphatidylcholines, lysophosphatidylcholines, sphingomyelins, …
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An in vitro and in vivo hepatotoxicity study of an aspalathin- rich green rooibos extract
… using both an in vitro human liver cell line (C3A) model, as well as a sub-chronic Sprague- Dawley rat model. Cultured C3A cells were treated with different concentrations of GRT ranging from untreated control, 0.1, 1, 10, 100 and 1000 μg/mL and incubated for 1 hour, 24 hours or 48 hours. …
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Metabolic Bioactivity of Athrixia phylicoides
… insulin-resistant C2C12 myocytes, C3A liver cells, 3T3-L1 adipocytes and in diabetic db/db mice. METHODS: Insulin resistance was induced in vitro using palmitic acid (750μM) for 16 hours followed by treatment with two concentrations (10 and 100 μg/ml) of the extract, in the presence …
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Assessment of chemical markers as surrogates for efficacy and safety of Rooibos extracts
… absorption in C2C12 murine skeletal muscle and C3A human liver cells, exposed to 10 μg/mL of the extracts. The effect of the green rooibos extracts (GRE’s) and reference extracts ARC 2 and GRT on glucose uptake and lipid accumulation was also tested in 3T3-L1 adipocytes. GRE’s were also tested …
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STRUCTURAL, FUNCTIONAL AND EVOLUTIONARY STUDIES OF ANTIMICROBIAL PEPTIDES
… that antimicrobial domains of the anaphylatoxin C3a are structurally and evolutionary conserved. Moreover antimicrobial activity is not governed by a single factor, but instead by a combination of net charge, amphipathicity and helicity. By utilizing a low number of amino acid substitutions at …
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VGF e stress ossidativo: alterazioni cellulari e modulazione nella Sclerosi Laterale Amiotrofica
… TLQP-21 on cerebellar granules. Recently, the C3a and gC1q have been identified as TLQP-21 receptors. Using the mouse motoneuronal cell line NSC-34 and the primary human fibroblast cell colture, we studied the expression and modulation of certain VGF peptides (VGFp) in ALS and oxidative stress …
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Trichloroacetimidates as Alkylating Reagents and Their Application in the Synthesis of Pyrroloindoline Natural Products and Synthesis of Small Molecule Inhibitors of Src Homology 2 Domain- Containing Inositol Phosphatase (SHIP)
… which mainly differ in the substitution at the C3a position. To provide rapid access to these natural products a diversity-oriented strategy was established via displacement of C3a-trichloroacetimidate pyrroloindoline. Carbon, oxygen, sulfur and nitrogen nucleophiles were all shown to undergo …
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