Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"C2C12 cells"”.
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Mechanisms of mTOR Signal Transduction Regulating Cell Growth and Differentiation
… mTOR is essential for miR-1 expression in C2C12 cells and in vivo in regenerating myofibers. It regulates the transcription of miR-1 through its upstream enhancers, and this regulation is at least partially mediated by the myogenic transcription factor MyoD. To delineate the pathway …
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Transcriptional Regulation of Neurogenic Atrophy-Induced Gene Expression by Muscle Ring Finger-1 and Myogenic Regulatory Factors
… of these genes were developed, transfected into C2C12 cells with or without a MuRF1 expression plasmid and monitored for differences in reporter gene activity. The MuRF1 and MAFbx reporters each showed repressed activity in cells ectopically expressing MuRF1 compared to cells that did not …
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Some bioactivity of triterpenes from stem bark of protorhus longifolia and their derivatives
… The effect of the compounds on glucose uptake in C2C12 muscle cells and 3T3-L1 adipocytes, and triglyceride accumulation in 3T3-L1 adipocytes was also investigated. The anticoagulant and anti-inflammatory activity was investigated using tail bleeding time assay and cotton pellet-induced granuloma …
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DEVELOPMENT OF A TISSUE ENGINEERED SKELETAL MUSCLE REPAIR CONSTRUCT FEATURING BIOMIMETIC PHYSICAL, CHEMICAL, AND MECHANICAL CUES
… observed on innervated skeletal muscle in vivo. C2C12 cells cultured on a 2D fibrin biomaterial and treated with neural (Z+) agrin exhibited clusters of acetylcholine receptors (AChRs). When agrin was presented bound to the surface of a carrier microparticle, this response was spatially …
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AMP- Activated Protein Kinase (AMPK) Activation for the Treatment of Mitochondrial Disease
… and mitophagy. We hypothesized that treating cells harbouring low numbers of mtDNA with an AMPK activator (5-Aminoimidazole-4-carboxamide ribonucleoside; AICAR) would ameliorate the decrease in ETC activity and improve mtDNA copy number. We developed myoblasts (C2C12 cells) depleted of mtDNA …
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Assessment of chemical markers as surrogates for efficacy and safety of Rooibos extracts
… to measure 2-deoxy-[3H]-D-glucose absorption in C2C12 murine skeletal muscle and C3A human liver cells, exposed to 10 μg/mL of the extracts. The effect of the green rooibos extracts (GRE’s) and reference extracts ARC 2 and GRT on glucose uptake and lipid accumulation was also tested in 3T3-L1 …
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Roles of Growth Hormone, Insulin-Like Growth Factor I, and Sh3 and Cysteine Rich Domain 3 in Skeletal Muscle Growth
… In the first study, the myogenic precursor cells, satellite cells, were isolated from cattle and allowed to proliferate as myoblasts or induced to fuse into myotubes in culture. GH increased protein synthesis without affecting protein degradation in myotubes; GH had no effect on …
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Role of Fra-2/AP-1 in Myogenic Progenitor Cells
… dynamic expression profiles during myogenesis in C2C12 cells, the expression profile of AP-1 is different among proliferative myoblasts, differentiating myoblasts, differentiated myotubes and reserve cells, indicating context-dependent spatial and temporal roles. We found that the expression …
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Burn Serum Stimulates Mitochondrial Fission in C2C12 Myoblasts
… in vitro. METHODS: Cultured murine myoblasts, C2C12 cells, w ere exposed to 10% rat serum isolated either from 40% total body surface area (TBSA) scald burn rats or control rats. Cells were then labeled with MitoTracker Green dye and live cell images were recorded by confocal microscopy. The …
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Uptake of Small EVs by Skeletal Muscle Myoblasts In Vitro and Subsequent Myogenic Response to Cargo
… cargo such as proteins and nucleic acids between cells, contributing to tissue function and repair. Small EVs released by skeletal muscle influence myogenesis in a cargo-dependent manner, with muscle-specific microRNAs (myomiRs) playing a central role in regulating the balance between myoblast …
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G protein-coupled receptor regulation: The role of protein interactions and receptor trafficking
… to elevate the level of cAMP in mouse myotube C2C12 cells, in a GPER1-dependent manner, but it is still not clear whether estrogen is the true agonist for GPER1. Taken together, the present thesis demonstrates the importance of protein-protein interactions and trafficking for B1R, B2R, and …
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Mechanical influence of pressure, topography, and diffusion on cell physiology and function
… incubation chambers which allow for the study of cells under hydrostatic pressure up to 150 psi. HepG2 cells are shown to have an altered cell cycle for pressures of 14.5 psi (100 kPa). In the second part of this thesis, the effect of topography and cellular mechanics is explored. This field of …
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Role of the Sh3 and Cysteine-Rich Domain 3 (STAC3) Gene in Proliferation and Differentiation of Bovine Satellite Cells
… and differentiation of bovine satellite cells. We isolated satellite cells from skeletal muscle of adult cattle and transfected them with STAC3 small interfering RNA (siRNA) or scrambled siRNA. Cell proliferation assays revealed that STAC3 knockdown had no effect on the proliferation rate …
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Genome-wide identification of enhancers, transcription factors, and mechanisms that control skeletal muscle differentiation in cattle
… process whereby the mononuclear muscle precursor cells myoblasts express skeletal muscle-specific genes and fuse with each other to form multinucleated myotubes. Comparing gene expression profiles in C2C12 cells, a widely used model of myoblasts, before and 6 days after induced myogenic …
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The Cellular and Molecular Mechanisms of Prostaglandin E₂ in Myogenesis
… survival and proliferation of hematopoietic stem cells, suggesting that PGE₂ signaling could be essential for tissue development and regeneration. Previous results have also indicated that inhibition of PGE₂ synthesis through the cycoloxygenase-2 (COX-2) pathway at an early stage of muscle …
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Investigation of the use of extracellular vesicles for the treatment of congenital muscular dystrophy
… blood. The possible benefits of these EVs on C2C12 cells were analysed. We also investigated the generation of a cell line of muscle cells overexpressing fkrp for packaging into EVs. These cells were cultured in a co-culture system with non-transfected cells and it was found that these …
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The Role of Ring1 and Rnf2 in Skeletal Muscle Regeneration
… and regenerative conditions. Satellite cells, a population of quiescent adult stem cells, maintain the regenerative potential of muscle well into adulthood, but the ability of the cells to maintain this is gradually lost as organisms age. In response to cues following injuries, or other …
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Regulation of Glut-4 Expression in Skeletal Muscle cells: The Roles of Nuclear Respiratory Factor-1 and calcium/calmodulin dependent protein Kinase
… NRF-1 and CaMK II regulate GLUT4 expression in C2C12 myocytes. We engineered C2C12 cells that overexpressed NRF-1 in response to doxycycline (Dox) using a Tet-On gene expression system and assessed the effects of NRF-1 overexpression on: a) MEF2A, GLUT4 and δALAS proteins by western …
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Development of a novel liquid chromatography based tool to study post-translational modifications
… study was the O-GlcNAc modification. Using the C2C12 mouse myoblast cell line, it was determined that the optimal conditions for producing lysates containing increased yields of O-GlcNAc modified proteins was to treat differentiated C2C12 cells with 10nM insulin, 12g/L glucose and 2mM of the …
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