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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 119 for “"C2C12"”.
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Burn Serum Stimulates Mitochondrial Fission in C2C12 Myoblasts
… in vitro. METHODS: Cultured murine myoblasts, C2C12 cells, w ere exposed to 10% rat serum isolated either from 40% total body surface area (TBSA) scald burn rats or control rats. Cells were then labeled with MitoTracker Green dye and live cell images were recorded by confocal microscopy. The …
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Decellularized Apium graveolens Scaffold for Cell Culture and Guided Alignment of C2C12 Murine Myoblast
The development of in vitro meat from validated tissue engineering techniques has emerged as a more sustainable and ethical method of meat production. To date, the expansion of cellular agriculture is believed to be dependent on four pillars: animal free scaffolds, serum free media, bioreactors and …
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Over-expression of NRF-1 in C2C12 myotubes increases GLUT4 content via a transcriptional cascade involving MEF2A
… and b) alters MEF2A-MEF2D dimer formation in C2C12 myotubes.
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An investigation into the novel effects of melatonin treatment on differentiated C2C12 myotubes in the absence and presence of pathology
… an in vitro approach, where differentiated C2C12 myotubes were either unexposed to pathology, or exposed to excess fructose (FRUC) or excess uric acid (UA) and MEL (in different dosages and treatment regimens). For paper 1, Healthy differentiated C2C12 myotubes exposed to MEL. Differentiated …
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The Cellular and Molecular Mechanisms of Prostaglandin E₂ in Myogenesis
… regeneration. Murine skeletal muscle cell line C2C12 was used as a model to test this hypothesis. The first part of the project is concerned with the role of PGE₂ in myogenic differentiation. Activation of PGE₂ signaling promoted C2C12 myogenic differentiation, which resulted from the …
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Part I characterization of MyoR in C2C12 mouse fibroblasts. Part II isolation and characterization of a novel class II bHLH transcription factor from the black widow spider, latrodectus hesperus
<p>PART I</p> <p>The basic helix-loop-helix (bHLH) family of transcription factors are involved in a variety of developmental processes. MyoR is the mouse homologue of the human transcription factor ABF-1 . MyoR is classified as a class II basic-helix-loop-helix transcription factor. In order to …
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The Role of Neuropeptide Y Y1R in Skeletal Muscle Lipid Metabolism
… skeletal muscle lipid metabolism. Rat L6, mouse C2C12, and human primary myotubes were incubated in 14C palmitate labeled fatty acid oxidation medium containing 80ng/mL, 250ng/mL, and 500ng/mL of NPY and for a three hour period. Experiments were repeated with the addition of 17mg/mL diprotin A to …
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Assessment of chemical markers as surrogates for efficacy and safety of Rooibos extracts
… to measure 2-deoxy-[3H]-D-glucose absorption in C2C12 murine skeletal muscle and C3A human liver cells, exposed to 10 μg/mL of the extracts. The effect of the green rooibos extracts (GRE’s) and reference extracts ARC 2 and GRT on glucose uptake and lipid accumulation was also tested in 3T3-L1 …
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The Change in the Concentration Levels of Extracellular Vesicles and of Cytokines in the circulatory blood system in Response to different exercise protocols
… and vesicle sizes. A mouse muscle cell line (C2C12) model was used to explore the effect of exercise on EVs concentration levels. As a measure of changes in differentiation of muscle cells the expression level of Myogenin in EV-treated C2C12 cells by plasma samples was determined by qPCR. The …
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Nutraceutical modulation of lipotoxicity and the development of a tool to monitor palmitate induced sub-cellular dysfunction.
… need to be further understood. In this thesis, C2C12 myotubes & S. cerevisiae are treated with tunicamycin or the saturated fatty acid palmitate (Chapter 3), to create models of ER stress and lipotoxicity, respectively. These models are then used in subsequent chapters to study the nutraceutical …
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Regulation of Glut-4 Expression in Skeletal Muscle cells: The Roles of Nuclear Respiratory Factor-1 and calcium/calmodulin dependent protein Kinase
… NRF-1 and CaMK II regulate GLUT4 expression in C2C12 myocytes. We engineered C2C12 cells that overexpressed NRF-1 in response to doxycycline (Dox) using a Tet-On gene expression system and assessed the effects of NRF-1 overexpression on: a) MEF2A, GLUT4 and δALAS proteins by western …
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Some bioactivity of triterpenes from stem bark of protorhus longifolia and their derivatives
… The effect of the compounds on glucose uptake in C2C12 muscle cells and 3T3-L1 adipocytes, and triglyceride accumulation in 3T3-L1 adipocytes was also investigated. The anticoagulant and anti-inflammatory activity was investigated using tail bleeding time assay and cotton pellet-induced granuloma …
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An in vitro study of the effects of free fatty acids on insulin mediated glucose uptake and metabolism by myocytes and fibroblast derived adipocytes
… and adipocytes. Materials and methods Mouse C2C12 and rat L8 cells were differentiated into myocytes and myotubules by serum deprivation, while mouse 3T3-L1 fibroblasts were differentiated into adipocytes by culturing in 3-isobutyl-1-methylxanthine (IBMX), dexamenthasone and insulin. Myocytes …
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Metabolic Bioactivity of Athrixia phylicoides
… metabolism in differentiated, insulin-resistant C2C12 myocytes, C3A liver cells, 3T3-L1 adipocytes and in diabetic db/db mice. METHODS: Insulin resistance was induced in vitro using palmitic acid (750μM) for 16 hours followed by treatment with two concentrations (10 and 100 μg/ml) of the extract, …
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Empleo de andamios tridimensionales celularizados estimulados mecánica y eléctricamente para la creación de tejido musculo-esquelético
… dos líneas celulares de mioblastos L6 y C2C12. Los andamios fueron estimulados mecánica y eléctricamente. Tras esto, se midió la viabilidad, morfología del citoesqueleto y funcionalidad de las fibras que se crearon. Los resultados mostraron que los andamios colágeno y alginato (82.33 ± …
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Uptake of Small EVs by Skeletal Muscle Myoblasts In Vitro and Subsequent Myogenic Response to Cargo
… Small EVs were generated from proliferating C2C12 myoblasts cultured in EV-depleted media and isolated using differential ultracentrifugation. Nanoparticle tracking analysis confirmed that the majority of isolated particles were within the expected size range (mean diameters below 200 nm). …
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Determination of the in vitro antidiabetic potential of a polyherbal commercial tea
… The effect of each extract on the viability of C2C12 myotubes, Ea.hy926 endothelial cells and human lymphocytes (HL) was determined using sulforhodamine B (SRB). The in vitro hypoglycaemic activity was assessed against α-amylase and α-glucosidase activity using 3,5-dinitrosalicylic acid (DNSA) …
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