Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"C2 Domain"”.
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Structural and Functional Studies of C2-Domain Proteins Involved In Neurotransmitter Release
… most of these regulatory proteins contain C2 domains, versatile protein modules with Ca2+-dependent and Ca2+-independent activities. However, the mechanism of regulation by these C2-domain proteins remains unclear. My research has focused on understanding the structural and functional …
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Ubiquitin Modulates Tollip's PtdIns(3)P Binding and Dissociates the Dimeric State of C-Terminal Cue Domain
… proteins are recognized by ubiquitin-binding domains, which are found in several effector proteins. In this study, we describe for the first time how ubiquitin controls the function of the Toll-interacting protein (Tollip), which is an effector protein in the innate immune signaling pathway …
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Structural characterization of synaptotagmin I
… The protein is composed of one trans-membrane domain, an unstructured linker followed by two C2A domains identified as C2A and C2B. Each C2 domain is composed of an 8 stranded β-sandwich joined by a 9 amino acid linker. The Ca+2 binding pocket is composed of three loops located at the apex …
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DISSECTING THE ROLE OF NEDD4 HECT E3 LIGASE AND ITS ISOFORMS IN CANCER
… the HECT family and is composed by a N-terminal C2 domain, four WW domains and the catalytic C-terminal HECT domain. Different studies evidenced a correlation between NEDD4 overexpression and tumor progression and in some cases its proto-oncogenic activity has been ascribed to the …
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Tobacco Phospholipase D β1: Molecular Cloning and Biochemical Characterization
… identity with PLDβ1 from tomato but lacks the C2 domain. PCR analysis confirmed insertion of the antisense fragment into the plants: three introns distinguished the endogenous gene from the transgene. PLD activity was assayed in leaf homogenates in PLDβ/g conditions. When phosphatidylcholine …
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Protonation and Membrane Protein Function
… (b) the Ca2+-activated membrane docking module (C2 domain) of human cytosolic phospholipase A2. Both of these biological systems were investigated using a multiscale approach, in which the results of the electrostatics calculations to determine protonation states are used to parameterize …
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Identification of host proteins targeted by the flax rust effector protein AvrL567
… revealed homology to known proteins and protein domains. Based on this analysis three interactors (iA1, iA2 and iA3) of AvrL567 were chosen for further investigation. iA1 is a plant protein that shows high sequence homology to the Arabidopsis thaliana cytokinin oxidase/dehydrogenase enzyme AtCKX7 …
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Identification of host proteins targeted by the flax rust effector protein AvrL567
… revealed homology to known proteins and protein domains. Based on this analysis three interactors (iA1, iA2 and iA3) of AvrL567 were chosen for further investigation. iA1 is a plant protein that shows high sequence homology to the Arabidopsis thaliana cytokinin oxidase/dehydrogenase enzyme AtCKX7 …
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Molecular and biochemical characterization of phospholipase D in cotton (Gossypium hirsutum L) seedlings.
… contained characteristic HKxxxxD catalytic domains, a PC-binding domain, a PIP2-binding domain and a C2 domain. In addition both cotton PLD β isoforms had a N-terminal "SPQY" rich domain which appeared to be unique to these PLDs. The three PLD δ isoforms, designated Ghpldδ1a, Ghpldδ1b and …
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The Group IVA Cytosolic Phospholipase A2/C1P Interaction and Its Role in Eicosanoid Synthesis and Inflammation
… which is located in the cationic β-groove of the C2 domain of cPLA2α. In this study we examined the effects of the genetic ablation of ceramide kinase (CERK) on eicosanoid synthesis, as CERK is the only known enzyme to produce C1P in mammalian systems. We utilized primary mouse fibroblasts (MEFs) …
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Biochemical and Genetic Studies on CC2D1A, a new NF-κB Activator and a Regulator of Synaptic Functions
CC2D1A is an evolutionarily conserved gene from worm to human. It belongs to a new protein family with four DM14 domains at the NH2 terminus and a C2 domain at the COOH terminus. The function of this protein family remains largely unknown. CC2D1A has been identified as a new NF-κB activator through …
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Regulation of PKCalpha and the role of PKC in neuroblastoma cell migration
… that autophosphorylation of two sites in the V5 domain of PKCalpha keeps the protein insensitive to diacylglycerol, presumably due to masking of the diacylglycerol-binding C1a domain. We have identified acidic amino acids in the V5 domain that, when mutated to alanines or lysines, renders …
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Analysis of Synaptotagmin-SNARE Complex Interactions by One-Dimensional NMR Spectroscopy
… and mediated primarily by the synaptotagmin C2B domain. Further, the polybasic region of C2B constitutes the primary binding site, while the two arginine residues at the bottom of the domain mediate additional interactions that lead to aggregation and precipitation. These results help clarify …
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Interfacial modulation of protein function explored through atomistic molecular dynamics simulation
… bilayers. Separately, simulations of C2 domain binding of synaptogamin (Syt) to the HMMM demonstrate clear differences in the binding properties between Syt isoforms, and explain the atomic origins of the observed differential kinetics between Syt-1 and Syt-7. Development of the HMMM …
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Measurement and Analysis of Calcium-Dependent Exocytosis in Giant Excised Membrane Patches
… affinity (KD ~71 uM) than expected for these C2-domain-containing proteins. I also developed a program for measuring and analyzing membrane capacitance. The program uses either sine waves or square waves to estimate cell parameters. Phase-sensitive detection is utilized in both cases. For …
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Regulation of Exocytosis by Syntaxin 4-Munc18c Complexes
… increase in Munc18c associating with the double C2 domain protein Doc2β. Disruption of Syn4-Munc18c upon tyrosine phosphorylation results in an increase in Syn4-SNARE complex formation and GSIS from beta cells. Similarly, tyrosine phosphorylation of Munc18c at Y219 and also Y521, disrupts its …
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Role of Ceramide-1-Phosphate as a Specific and Potent Activator of Group IVA Cytosolic Phospholipase A2 Alpha
… to the cationic β-groove (R57, K58, R59) of the C2 domain of cPLA2α. In this regard, mutants of this region of cPLA2α were generated ((R57A/K58A/R59A), (R57A/R59A), (K58A/R59A), (R57A/K58A), (R57A), (K58A), and (R59A)) and examined for C1P affinity by surface plasmon resonance (SPR). The triple, …
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Signalling mechanisms of the tyrosine phosphatase SHP2 upstream of Ras
… and mapped the interaction between the SHP2 PTP domain and the C-terminus SH3-GUK domains of DLG5 using transiently expressed DLG5 truncated constructs. The RASAL2 N-terminal C2 domain appears to be key in binding directly to the SHP2 PTP, according to interaction mapping in vitro. Both …
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Crosstalk Between Calcium Signaling and Lipid Metabolism at Endoplasmic Reticulum-Plasma Membrane Junctions
… synaptotagmin-like protein 1 (E-Syt1), a C2 domain-containing ER membrane protein. E-Syt1 detects the increase in cytosolic Ca2+ via its C2C domain and translocates from the bulk of ER to ER-PM junctions to enhance ER-to-PM connection. This in turn facilitates the recruitment of Nir2, an …
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Localisation and function of phosphoinositide-specific phospholipase C in Sacchromyces cerevisiae
… association is required for intact PH, X-Y and C2 domain – disruption in any of these domains would result in failure of such association. Hence, higher phosphorylated inositol phosphates seem to influence Plc1p’s localisation, and a physiological feedback cycle of regulation probably exists …
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