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Showing 1 to 20 of 21 for “"C. glutamicum"”.
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Metabolic engineering of C. glutamicum for amino acid production improvement
… in the bacterial strain Corynebacterium glutamicum that is employed for the fermentative production of various amino acids such as lysine. The main goal of the research presented here was the application of metabolic and genetic engineering tools in order to investigate the role of the …
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The genetics of mycolic acids biosynthesis in Corynebacterium glutamicum
Corynebacterium glutamicum and Brevibacterium are of great importance to the fermentation industry. They have potential for application in foreign protein expression. This research has been focused mainly on strain improvement and development of technique to overcome the barriers that effect …
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L-arginine overproduction in Corynebacterium glutamicum ATCC 13032
Corynebacterium glutamicum is widely used for the commercial production of a variety of amino acids, including L-lysine, L-glutamate and L-threonine. With the exception of Larginine, the biosynthesis and regulation of most of these compounds in this bacterium are relatively well characterised in …
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Channel-forming proteins in the cell wall of amino acid-producing Corynebacteria
Corynebacterium glutamicum is together with C. callunae and C. efficiens a member of the diverse group of mycolic-acid containing actinomycetes, the mycolata. These bacteria are potent producer of glutamate, lysine and other amino acids on industrial scale. The cell walls of most actinomycetes …
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Biochemical and molecular characterisation of cell wall glycosyltransferases in Mycobacterium tuberculosis
… to the non-pathogenic bacterium Corynebacterium glutamicum. The availability of their genome sequences has enabled the utilisation of C. glutamicum as a model for the identification and study of essential mycobacterial genes involved in the synthesis of cell wall components such as lipomannan …
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Molecular and biochemical characterisation of novel glycosyltransferases in Mycobacterium tuberculosis
… and the closely related species Corynebacterium glutamicum, with features common to the GT-C superfamily of glycosyltransferases. A novel arabinofuranosyltransferase, AftC, was deleted from both M. smegmatis and C. glutamicum and shown to be an internal branching α(1→3) …
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Nitrogen metabolism in Corynebacterium glutamicum ATCC 13032
Corynebacterium glutamicum is extensively used for the commercial production of a host of amino acids including lysine, glutamate, and threonine. Consequently, much research has been directed at analyzing nitrogen metabolism in this bacterium. In particular, our research focused on investigating …
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Glycogen metabolism in Corynebacterium glutamicum ATCC 13032
Corynebacterium glutamicum is a Gram-positive facultative aerobe particularly known for its industrial application in the synthesis of amino acids, such as L-glutamate and Llysine. The central metabolic pathways of this organism has been an area of much research by many groups. Linked to glycolysis …
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Development and Application of a Microfluidic Cultivation Platform for the Analysis and Control of Industrially Relevant Synthetic Microbial Co-Cultures
… of an L-lysine producing Corynebacterium glutamicum strain and an L-lysine auxotrophic C. glutamicum variant in spatially separated but nanochannel-connected cultivation chambers at the single-cell level. L-lysine secreted by the L-lysine producer could be taken up by the L-lysine …
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Untersuchung des Argininmetabolismus und dessen Regulation in Corynebacterium glutamicum ATCC 13032
… wirtschaftlichem Interesse. In Corynebacterium glutamicum, einem industriell bedeutsamen Produzenten von Aminosäuren, gibt es ein Argininoperon, welches aus den Genen argCJBDFRGH besteht und dessen Transkription ausgehend von einem Promotor stromaufwärts von argC durch ein leaderless Transkript …
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A streamlined Workflow for Transposon Mutagenesis in Corynebacterium glutamicum Based on Third-Generation Sequencing and its Application for Advanced Genetic Screens
… and insertion sequence(IS)-free *Corynebacterium glutamicum* platform strain CR101 for an efficient integration system, a transposon mutagenesis system based on the endogenous IS*Cg1* transposase and a streamlined workflow for transposon mutant library preparation and sequencing using Oxford …
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Development and application of a microfluidic single-cell cultivation device to simulate dynamic environmental conditions for Corynebacterium glutamicum
… relevant bacterium *Corynebacterium glutamicum* was used to investigate the influence of different fluctuating environmental conditions on its growth and morphology. An initial study of nutrient oscillations with intervals between 10 seconds and 1 hour showed significant drops in the …
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Microbial single-cell growth kinetics and heterogeneity in nutrient-limiting environments
… lysine-producing *Corynebacterium glutamicum Lys4ΔptsF*(pVWEX1-crimson) and a fructose-utilizing lysine auxotrophic *Escherichia coli Lys1*(pVWEX1-gfpUV) strain. Here, a growth kinetic has been developed that allows for growth behavior and performance prediction depending on initial …
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Design, manufacture, and application of DNA microarrays to study gene expression phenotypes of lysine-producing Corynebacterium glutamicum
Corynebacterium glutamicum partial genome DNA microarrays were constructed that were capable of assaying the transcriptional profile of the genes of pathways involved in central carbon metabolism and lysine biosynthesis. It was found that to ensure arrays of high quality, protocols applying the …
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Phosphoenolpyruvat-Carboxylasen in Solanum tuberosum : Charakterisierung und Expression
… (PEPC)-gene from Corynebacterium glutamicum, plants were produced in which the PEPC activity was detected to be 6 times higher than in the parental plants. Similarly, F1-descendants were obtained in which the phosphoenolpyruvate synthase gene from E.coli, as well as the C. …
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Chemical Probes and Strategies to Study Mycobacterial Cell Envelope Assembly
… enrichment of glycolipids across Corynebacterium glutamicum, Mycobacterium smegmatis, and Mtb. This strategy provides a platform to study glycolipid dynamics in wild-type cells, a task previously hindered by the lack of selective labeling tools. In Chapter 4, we further interrogate endogenous …
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Characterization of Bacterial Transcriptional Regulatory Networks through Genome-Wide in vitro Run-Off Transcription RNA-Seq (ROSE)
… conducted on the genomic DNA of Corynebacterium glutamicum ATCC 13032 using purified C. glutamicum RNAP core enzyme, which was reconstituted with both σA and σB. A comparison between the RNAP core enzyme and RNAP-σA revealed that only well-conserved promoter motifs were present in the RNAP-σA …
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A new method to quantify the CO 2 sensitivity of micro-organisms in shaken bioreactors and scale up to stirred tank fermentors
… and lysine product in the fermentation of C. glutamicum DM 1730 was found. Furthermore, the experimental results on Arxula adeninivorans LS3 and Hansenula polymorpha (WT ATCC 34438 and RB11-FMD-GFP) indicated that the CO2 had no effect on these microorganisms. Pseudomonas fluorescens DSM 50090 …
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Untersuchungen zur L-Serin-Bildung mit Corynebacterium glutamicum
… position in the metabolism of corynebacterium glutamicum. This is especially because of the reaction catalyzed by the serine hydroxymethyltransferase (SHMT) in which serine is degraded to glycine and a C1 unit. It was an aim of this work to analyze the formation of the pharmaceutical important …
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Modeling based process development of fed-batch bioprocesses: L-valine production by Corynebacterium glutamicum
… L-valine production by a genetically modified C. glutamicum. With four experiments, a model was developed and selected. Two different model discriminating criteria were used for the design of these experiments. The selected model was used for calculation of optimal feed trajectories for …
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