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Showing 1 to 20 of 26 for “"C. Beijerinckii"”.
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Genetic Characterization and Manipulation of Solvent -Producing Clostridia
The sol operons from Clostridium beijerinckii NCIMB 8052 and C. beijerinckii BA101 were cloned and sequenced to determine if differences at the sequence level were responsible for the increased butanol production exhibited by C. beijerinckii BA101 relative to the wild type C. beijerinckii NCMIB …
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Examination of Glucose Transport in Clostridium Beijerinckii NCIMB 8052 and the Solvent Hyper-Producing Mutant BA101
Glucose uptake and accumulation by Clostridium beijerinckii BA101, a butanol hyper-producing mutant was examined during various stages of growth. Glucose uptake in C. beijerinckii BA101 was repressed 20% by 2-deoxyglucose (2-DG) and 25% by mannose, while glucose uptake in C. beijerinckii 8052 was …
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A systems biology approach to understanding and improving butanol production in clostridium beijerinckii
… fuel additive or replacement. Clostridium beijerinckii is an attractive microorganism for butanol production because it (1) naturally produces the highest recorded butanol concentrations as a byproduct of fermentation; and (2) can co-ferment pentose and hexose sugars (the primary products …
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Molecular Characterization and Utilization of the CAK1 Filamentous Viruslike Particle Derived From Clostridium Beijerinckii NCIMB 6444
… Thermoanaerobacterium polysaccharolyticum in C. beijerinckii NCIMB 8052 and the sol operon of C. acetobutylicum DSM 792 in C. beijerinckii SA-2 when using pYL102E indicates that pYL102E can be used as an E. coli-C beijerinckii shuttle vector. This observation indicates the utility of CAK1-derived …
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Nitrogen Metabolism and Solvent Production in Clostridium Beijerinckii Nrrl B593
… Clostridium acetobutylicum and Clostridium beijerinckii, are nitrogen-fixing organisms and both nitrogen-fixation and alcohol production (n-butanol, isopropanol and ethanol) are reductant-dependent processes, the effect of nitrogen fixation on the onset and progression of solvent production …
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Development of a reporter system for the study of gene expression for solvent production in Clostridium beijerinckii NRRL B592 and Clostridium acetobutylicum ATCC 824
… potential reporter gene was found in Clostridium beijerinckii NRRL B593, which contains an adh gene encoding a primary-secondary alcohol dehydrogenase and this adh gene is not present in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NRRL B592. The adh gene was cloned into the E. …
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The Cloning of a Putative Regulatory Gene and the sol Region from Clostridium beijerinckii
… From an isopropanol-producing strain Clostridium beijerinckii NRRL B593, a gene which encodes a putative NtrC-like regulatory protein was cloned and sequenced. The gene codes for a polypeptide of 632 amino acids and has been designated the stc gene. Expression of the stc gene was confirmed by …
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The characterization of Clostridium beijerinckii NRRL B592 cells transformed with plasmids containing the butanol-production genes under the control of constitutive promoters
Clostridium beijerinckii is a spore-forming, obligate anaerobe that is capable of producing butanol, acetone and isopropanol. These industrial chemicals are traditionally known as solvents. The regulation of solventogenic fermentation is linked to the onset of sporulation, so that by the time the …
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Nitrogen metabolism and butanol production by South African clostridium beijerinckii and clostridium saccharobutylicum strains
… A collection of 19 solventogenic Clostridium beijerinckii and 11 Clostridium saccharobutylicum strains isolated from the National Chemical Products (NCP) ABE fermentation plant in Germiston, South Africa, were classed according to species by a quick species-specific colony PCR and by …
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Transcriptomic analyses of Clostridium beijerinckii NCIMB 8052 during transition from acidogenesis to solventogenesis and under butyrate supplemented conditions
… as an industrial chemical feedstock. Clostridium beijerinckii is among the prominent species for biobutanol production as it demonstrates a broad substrate range for growth and solvent production. Although the transcriptome structure and transcriptional profiling are essential for understanding …
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Production of Biobutanol from inulin-rich biomass and industrial food processing wastes
… for butanol production. The ability of C. beijerinckii NCIMB 8052 and C. acetobutylicum ATCC 824 to ferment food processing wastes was tested in batch-fermentation mode. C. acetobutylicum ATCC 824 gave the highest ABE yields on the media with milk dust powder (10.25 g/L), inedible dough …
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Enhanced biohydrogen production and substrate utilization by co-culture fermentation with reduced extracellular electron shuttles
… substrate utilization. A co-culture system of C. beijerinckii and G. metallireducens with extracellular electron shuttles was developed and evaluated for improved biohydrogen production. To enhance biohydrogen production, Clostridium beijerinckii was co-cultured with Geobacter metallireducens in …
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Production of 1-butanol and butanol isomers by metabolically engineered Clostridium beijerinckii and Saccharomyces cerevisiae
… by solventogenic clostridia such as Clostridium beijerinckii. Although the ABE fermentation is considered as a promising process for butanol production, current drawbacks in the ABE fermentation include low titer, yield, and productivity of solvent production that needs to be improved to achieve …
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Studies on the sucrose utilization system of Clostridium beijerinckii NCIMB 8052
… of the sucrose utilization system of Clostridium beijerinckii NCIMB 8052. The C. beijerinckii sucrose utilization operon, scrARBK, was cloned in three stages. In the first stage a C. beijerinckii genomic library was screened in Escherichia coli for clones able to utilize sucrose. Using this …
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Studies on the regulation of nitrogen metabolism in clostridium acetobutylicum NCP 262 and clostridium beijerinckii NCIMB 8052
… to find an alternative to heterologous host. C. beijerinckii NCIMB 8052, which is closely related to C. acetobutylicum NCP 262, was evaluated as a model organism for the study of nitrogen metabolism in the latter. It was found that organic nitrogen in the form of cas amino acids was preferred …
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Enhancing energy recovery from industrial wastewater using microbial fuel cells
… of Shewanella oneidensis and Clostridium beijerinckii, investigated here for the first time, gave a power production of 87 mW m-2 compared to 48 mW m-2 for S. oneidensis alone or 60 mW m-2 for C. beijerinckii alone. Substrate degradation was also improved significantly from 20% (S. …
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Metabolic network reconstruction and genome-scale model of butanol-producing strain clostridium beijerinckii ncimb 8052
… and potential fuel additive or replacement. C. beijerinckii is an attractive microorganism for strain design to improve butanol production because it (i) naturally produces the highest recorded butanol concentrations as a byproduct of fermentation; and (ii) can co-ferment pentose and hexose …
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Genetic Manipulation and Characterization of Solvent-Producing Clostridia
… growth media. When used in combination with C. beijerinckii, BA101, this newly developed medium holds promise to increase the cost-effectiveness of the ABE fermentation. (Abstract shortened by UMI.).
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Investigation of carbon catabolite repression in Clostridium beijerinckii NCIMB 8052
… ccpA mutant strain. The sucrose operon from C. beijerinckii NCIMB 8052, scrARBK, has been characterised at the physiological and genetic levels with the ScrR repressor found to negatively auto-regulate the operon.
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Genetic and Physiological Characterization of Clostridium Beijerinckii BA101 and NCIMB 8052
… also able to stabilize solvent production by C. beijerinckii BA101, a hypersolvent producing mutant derived from NCIMB 8052. Moreover, BA101 demonstrated a greater increase in solvent production than NCIMB 8052 when acetate was added into MP2 medium. During the exponential growth phase, the …
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