Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 424 for “"C-terminus"”.
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A study of the C-terminus in prestin by designing cleavable constructs
… proteins, which all have a large cytosolic terminus containing a conserved region known as the STAS domain (Sulfate Transporters and Anti-Sigma factor antagonist), whose function re- mains unknown. In this work, we engineered prestin by inserting three types of short peptides, flexible, …
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The Role of the C-terminus in Bacillus subtilis inorganic pyrophosphatase activity
… into the role of a signature sequence near the C-terminus of B. subtilis enzyme was commenced. Two truncated variants and a series of site specific mutants were produced and the effect of the mutations on enzyme activity, stability and multimerization was analysed. Overall, there is no evidence …
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Isolation of the Cytosolic C-Terminus Segment of Mammalian Kca Channel a Subunit
<p>The last 20 years of research into ion channels has unraveled unexpected features of the BK<sub>ca</sub> channel as a rheostast for cell activity in excitable tissues and homeostatically fine tuning many biological activation processes in the cell. Great attention has been dedicated to the …
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Die Rolle des C-Terminus von Env in der HIV-1-Infektion und -Replikation
… in die Rolle des ungewöhnlich langen C-Terminus von HIV-1-Env (Env-CT) in der Infektion und Replikation zu gewinnen. Es ist bisher nicht bekannt, aus welchen Gründen die Länge des Env-CTs in vivo streng konserviert ist und Virusmutanten mit trunkierten Env-CTs in vitro einen …
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C-terminus of Hepatitis B virus RNase H Domain is Important for HBV replication
… to 826 (800SRPLLRLPFQPTTGRTSLYAVSPSVPS826) in C -terminus of the RNase H domain are required to complete HBV replication. HBV P protein mutants in which single amino acid residue was substituted were examined for the rescue of HBV replication. Among these mutants tested, L806T mutant P protein …
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Der C-Terminus des antiapoptotischen Bcl-2 Familienmitgliedes A1 reguliert Stabilität und Funktionalität des Proteins
… instabilen chimären Protein. Somit scheint der C-Terminus von A1 sowohl notwendig als auch hinreichend für die kurze Halbwertszeit des Proteins zu sein. Die meisten kurzlebigen Proteine werden über den proteasomalen Signalweg degradiert. Dies scheint auch für A1 zu gelten, da seine Halbwertszeit …
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Alternative splicing and RNA editing of the Complexin C-terminus regulates neurotransmitter release in Drosophila
Chemical synaptic transmission is an essential and highly regulated step in neuronal communication. Changes in the strength of synaptic transmission mediate several forms of plasticity associated with learning and memory. Functional regulation of Complexin (Cpx) provides an entry point for altering …
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TREX1 C-Terminus Regulates Oligosaccharyltransfererase to Prevent the Accumulation of an Endogenous Bioactive Disaccharide Associated with Autoimmune Disorders
… difference invoked the hypothesis that TREX1 C-terminus engages in the interaction with ER-residing proteins and such interaction could contribute to the C-terminus associated diseases. Through investigating of this possibility, I identified that TREX1 interacts with the …
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Structural Characterization of the C-terminus of the Cochlear Motor Protein Prestin: Intrinsic Disorder Linked to Voltage Sensor Function
… unknown. Previous research has tied prestin’s C-terminus (residues 499-799) to the voltage-dependent events that occur in the membrane. The folding and biochemical properties of prestin’s C-terminus were characterized. This information was used to probe the function of the C-terminus.</p> <p> …
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Identification and characterization of human plasma prekallikrein-prolylcarboxypeptidase interaction sites
… corresponding to the last 10 amino acids of PK C-terminus inhibited PRCP1 activity. Three recombinant PKs (rPK), which are only distinct from each other at the extreme C-terminus, exhibited elevated susceptibility to PRCP1-induced activation. Our results demonstrate that the PK C-terminus …
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FtsZ Protofilament Curvature is the Opposite of Tubulin Rings
… the membrane tether, which exits from the C terminus of the globular FtsZ, would have to be on the outside of the curved pf. However, it is well established that tubulin rings have the C terminus on the inside of the ring. Could FtsZ and tubulin rings have the opposite curvature? In the …
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Expression, purification and characterization of the structural properties of recombinant Pysp1 and Pysp2 spidroins
… block repeats. Moreover, because the PySp C-terminus amino acid residues are distinct from MaSp C-terminus and the morphology of these glands is different, we hypothesized that PySp C-terminal domains form distinct secondary structures. The MaSp C-terminus has been shown to regulate the silk …
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Insights into the regulation of the CRL4D̳T̳L̳ ubiquitin ligase
… DTL contains multiple WD40 repeats in the N-terminus that are very highly conserved and a less conserved C-terminus that may have important regulatory function. We characterize DTL regulation during the cell cycle: DTL itself is degraded in an ubiquitin-dependent manner and degradation is …
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A Critical Role Of Cxcr2 Pdz Motif-Mediated Interactions In Endothelial Progenitor Cell Homing And Angiogenesis
… PSD-95/DlgA/ZO-1 (PDZ) motif at its carboxyl terminus. The PDZ motif has been reported to regulate cellular signaling and functions. Here we investigated the potential role of the PDZ motif in CXCR2-mediated EPC motility and angiogenesis. We have found that introducing exogenous CXCR2 …
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OspC in the pathogenesis of Borrelia burgdorferi
… interconnected by 6 loops, and an N- and C-terminus. Not much is known as to how the OspC structure relates to the function of the lipoprotein and/or possible regulation. The up-and down-regulation of OspC is achieved by the rpoS alternative sigma factor and by an unidentified repressor, …
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IAP antagonist Grim mediates cell death through divergent pathways
… an IBM-independent manner, involving Grim’s C-terminus for translational repression. We discovered that Grim is a target for caspase-mediated cleavage at Grim’s C-terminus (Asp132). Grim loses its only lysine (Lys 136) after cleavage, but acquires a longer half-life due to inability of DIAP1 to …
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Probing spatial and subunit-dependent signalling by the NMDA receptor
… Moreover, I tested the hypothesis that the Cterminus of GluN2A and GluN2B are essential in controlling the developmental switch of GluN2 subunits utilizing knock-in mice whereby the C-terminus of GluN2A is replaced with that of GluN2B. Surprisingly, the exchange of the C-terminus does not …
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Studies towards the in vivo inhibition of oligosaccharyl transferase
… by cells in culture. Those that contained the C-terminus ER retrieval sequence were concentrated in the ER, while the inhibitors without the ER retrieval sequence were found distributed throughout the cell. Nevertheless, inhibitors with an amide C-terminus were internalized with more ease that …
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Roles of the Chromatin Insulator Protein Cp190 in the Development of Drosophila melanogaster
… and homozygous for the Cp190 mutation (deleted C-terminus) were stained in order to view nuclear migration and to determine their developmental stage. To establish whether the homozygous mutants were able to develop into larvae, a GFP signal in heterozygous flies was viewed. Results showed that …
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Ring-Opening of Aziridine -2 -Carboxamides by Carbohydrate C1-O Nucleophiles. Stereoselective Preparation of O-Linked Glycopeptides
… (ABn) moiety was developed as a new C-terminus secondary amide protective group for carboxylic acids, which allows for oxidative C-terminus extension of amino acids following the carbohydrate-aziridine coupling reaction. A new protocol for deprotection of p-nitrobenzenesulfonamides with …
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