Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 162 for “"C-terminal domain"”.
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Structural characterization of the C-terminal domain of human nucleophosmin, NPM1
… OUT STRUCTURAL STUDIES ON THE 53 RESIDUE C-TERMINAL DOMAIN OF HUMAN NPM1, WHICH COMPRISES AN AROMATIC RICH REGION, CONTAINING TWO TRYPTOPHAN RESIDUES. USING NMR, WE FOUND THAT THE C-TERMINAL DOMAIN OF NPM1 IS MADE UP OF 3 ALPHA-HELICES, EACH ENCOMPASSING 10-12 AMINO ACIDS. IT FORMS A …
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A Functional Analysis of the RNA Polymerase II Large Subunit C-Terminal Domain
Doktorarbeit zur Untersuchung der grossen Untereinheit des RNA polymerase II
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The conserved C-terminal domain of spider tubuliform spidroin 1 contributes to extensibility in synthetic fibers
… 1 fusion proteins that contain the conserved C-terminal domain exhibit increased extensibility and toughness when compared to the identical fibers spun from fusion proteins lacking the C-terminus. Mechanical analyses reveal that the properties of synthetic tubuliform silks can be modulated by …
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Expression and Purification of the C-Terminal Domain of Porcine Epidemic Diarrhea Virus (PEDV) S1 Protein
… into the host cell. The S protein is made of two domains, the S1 domain which allows for protein interactions between the virus and the host cell, and the S2 domain which allows for membrane fusion. Because of the S1's role in protein interaction, it is often the target of potential vaccines. …
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Structural principles governing kinase specificity towards RNA polymerase II C-terminal domain and their impact on transcription
Transcription, one of the major processes in the cell, is the conversion of genetic information (DNA) into a temporarily stored information (RNA). To orchestrate, selectively regulate and control this conversion, cells have evolved a variety of tools – the most interesting being the CTD of Pol II. …
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Structural and functional studies of the C-terminal domain of human apolipoprotein A-I: Limited proteolysis and deletion mutagenesis
Limited proteolysis was used to study the domain structure and to produce a large N-terminal fragment of human apolipoprotein A-I (apoA-I). Digestion of reconstituted high density lipoprotein (rHDL) prepared with apoA-I and dipalmitoyl phosphatidylcholine (DPPC) or palmitoyloleoyl PC (POPC) by …
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Structural and functional role of the extracellular loops and C-terminal domain of the voltage-gated sodium ion channel
… The α-subunit of Nav consists of four homologous domains (DI-DIV), each consisting of six transmembrane helices (S1-S6). Helices S5-S6 of each domain forms the lining of the central pore through which sodium ions (Na+) enters the cell upon channel activation. Helices S1-S4 of each domain form the …
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Characterization of the Association of mRNA Export Factor Yra1 with the C-terminal Domain of RNA Polymerase II in vivo and in vitro
<p>The unique C-terminal domain (CTD) of RNA polymerase II (RNAPII), composed of tandem heptad repeats of the consensus sequence YSPTSPS, is subject to differential phosphorylation throughout the transcription cycle. Several RNA processing factors have been shown to bind the appropriately …
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Role of the C-terminal domain of the <font face = "symbol">a</font> subunit of RNA polymerase in transcriptional activation of the <i>lux</i> operon during quorum sensing
… purified to date, an autoinducer-independent N-terminal truncated form of LuxR, LuxR<font face = "symbol">D</font>N, was used for <i>in vitro</i> studies. Single-round transcription assays were performed using reconstituted mutant RNAPs in the presence of LuxR<font face = "symbol">D</font>N, and …
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Characterization of Leucyl -Trna Synthetase From Escherichia Coli and Saccharomyces Cerevisiae in Aminoacylation, Amino Acid Editing, and Protein-Dependent RNA Splicing
… mutagenesis, we characterized the C-terminal domain of LeuRSs from different origins to understand its role. The results demonstrated that the native environment of the enzyme influenced the functional role of the unique C-terminal domain of both LeuRSs. These combined biochemical …
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Strengths and Weaknesses of Hybrid TPR Technology for Obtaining Structural and Mechanistic Insights into TPR Proteins
… repeated pack into a superhelical structure. TPR domains are frequently found mediating protein-protein interactions, often through a central groove. One protein complex bearing numerous TPR repeats is the Anaphase Promoting Complex (APC). The anaphase-promoting complex (APC) is a multi-subunit …
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Study of the Gain-of-Function Mutant p53 and PARP1 in Triple-Negative Breast Cancer
… MDA-MB-468, we examined the role of the R273H C-terminal basic domain, in the mtp53-PARP-MCM axis. We hypothesized that the C-terminal domain in mutant p53 R273H facilitates chromatin recruitment of PARP1. This work compared CRISPR-Cas9-edited MDA-MB-468 cells with truncations in the C-terminal …
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Development of Mono-de-N-acetylated β-(1→6)-N-acetyl-D-glucosamine Substrates for Characterization of PgaB Hydrolase Activity
… dPNAG in Gram-negative bacteria. PgaB is a two-domain periplasmic protein that processes PNAG prior to its export to the extracellular space. ΔpgaB strains have an impaired biofilm-forming phenotype, making PgaB a potential therapeutic target. The N-terminal domain of PgaB is a family 4 …
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Molecular alterations of AMPA receptors and their effects on hippocampus dependent tasks
I investigated the role of C-terminal domain modifications in GluR-A mediated behavioral function using several hippocampus dependent behavioral tasks, ranging from neophobia to spatial reference memory, in a variety of genetically modified mice. Studies using GluR-A knock-out (GluR-A-/-) mice …
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Structural and mechanistic investigation towards the regulation of M.tb isocitrate lyase
… acetyl-CoA and propionyl-CoA) to its C-terminal domain. As acyl-CoA is produced when fatty acids are used as a carbon source, this work showed that ICL2 is a gatekeeper in regulating carbon flow towards the glyoxylate shunt. Furthermore, by capitalising on the recently-solved M. tb ICL2 …
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Modification and Translocation of the Biofilm Exopolysaccharide Poly-β(1,6)-N-acetyl-D-glucosamine
… epidermidis, respectively. E. coli PgaB is a two-domain outer membrane lipoprotein that continually associates with PNAG throughout periplasmic translocation. Studies described herein suggest that PgaB binds and deacetylates PNAG along the cleft between the N- and C-terminal domains in a …
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The enzymatic function of the CCR4 protein
… specifically interacted with both N- and C-terminal domains of the NOT1 protein, suggesting that it lies adjacent to NOT1. Genetic analysis indicated that CAF40 and CAF130 share few phenotypes with the rest of the CCR4-NOT complex genes, consistent with the physical separation of CAF40 and …
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New molecular and cellular aspects of mutant calreticulin in Myeloproliferative Neoplasms
… CALR mutations affect the nature of the C-terminal domain of this protein, its sub-cellular compartmentalisation and its Ca2+ buffering activity during megakaryocyte hyperplasia. Additionally, this study establishes a new cellular model to investigate megakaryocyte differentiation in …
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Solution Structure of the Apocalmodulin/neuro-P Complex
… (Neurop ) derived from the calmodulin-binding domain of the neural-specific protein neuromodulin has been studied by nuclear magnetic resonance (NMR). This complex is in fast exchange with its components on the NMR timescale, and the dissociation constant for the complex is in the micromolar …
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VALIDATION OF CANDIDATE GENES IN RESPONSE TO VERSICAN MANIPULATION IN DEVELOPING SYNOVIAL JOINTS
… It has four functional-modules: the N-terminal domain, the C-terminal domain, and GAG-[alpha], and GAG-[beta] chondroitin sulfate attachment regions. The N-terminal domain is also known as the G1 domain and the C-terminal domain is also known as the G3 domain (Kimata et al., 1986 and …
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