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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"Bulked Segregant Analysis"”.
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Characterization of Resistance to Gray Leaf Spot [Pyricularia Grisea (Cooke) Sacc.] in Perennial Ryegrass and Tall Fescue
The tall fescue population was analyzed using bulked segregant analysis (BSA) with random amplified polymorphic DNA (RAPD) markers to identify DNA markers associated with resistance to gray leaf spot. Although polymorphic bands were identified between the resistant and susceptible bulks, these …
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Estudo de herança e de marcadores moleculares para teor de ácido Iinolênico em soja
… precisão, os individuos desejados na população segregante, em gerações precoces. A metodologia “Bulked Segregant Analysis” foi utilizada para identificar marcadores RAPD Iigados a genes que determinam teor de ácido Iinolênico. Os 1000 “primers” aleatórios testados não detectaram nenhum …
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Broadening the wheat gene pool for stem rust resistance through genomic-assisted introgressions from Aegilops tauschii
… of BC[subscript]2 mapping populations. Bulked segregant analysis of BC[subscript]2F[subscript]1 genotypes at 70 SSR loci across the D genome identified the chromosome locations of stem rust resistance genes and facilitated genetic mapping. Using this approach, TTKSK resistance from …
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Approach to the identification of sex-determining genes in the tilapia genome by genetic mapping and comparative positional cloning
… between sex chromosomes.</p><p>To facilitate QTL analysis of these traits, a genetic linkage map was constructed from hybrids between Oreochromis aureus and O. niloticus using CRIMAP. The linkage map consists of 25 linkage groups with about 500 genetic markers spanning 2345 cM. Using the …
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Physiological and genetic mechanism(s) of cold acclimation in Rhododendron
… with LFT differences among cold-acclimated F2 segregants. Moreover, its expression increased as LFT increased with both chronological age and developmental phase-change of wild and cultivated plants. The presence or absence of the 25-kDa dehydrin may serve as a genetic marker for 'super' CH in …
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Physiological Bases and a Novel Genetic Determinant of Water-Use Efficiency (WUE)
… WUE and/or biomass using a YK x SH F<sub>2</sub> bulked segregant analysis. Candidate genes were screened in <em>A. thaliana</em> mutants. <em>TAF10</em> encodes one of the thirteen TATA-box binding protein associated factor (TAF) constituents of the TFIID complex and a loss-of-function mutant …
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The agronomic and molecular characterisation of Rht8 in hexaploid wheat
… was further fine-mapped using an RNA-Seq enabled bulked segregant analysis method, as well as utilising SNP-platforms and emerging Triticeae genomic resources to identify molecular markers. Rht8 was reduced to a 1.015 cM genetic interval and syntenic intervals of 1.34 Mb on rice chromosome 4, 1.36 …
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Genetic and Molecular Analysis of the Rj(7) Gene in Soybean Using Pcr-Based Technologies
… hypocotyl as a phenotypic marker for allelism analysis, it was concluded that the NOD2-4 and NOD3-7 mutants carried the same mutant allele at the rj7 locus. There was not an independent mutant locus in NOD2-4 as speculated previously. Simple sequence repeat (SSR), random amplified polymorphism …
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RAPD markers for ascochyta resistance, phylogenetic studies and cultivar identification In lentil
… with high resolution were developed for lentil. Bulked segregant analysis was used to produce four bulks of DNA from resistant vs. susceptible F2 plants in each of these two populations which were then screened for RAPD markers using 400 random oligonucleotide primers. One RAPD marker, …
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Phytophthora nicotianae: Fungicide Sensitivity, Fitness, and Molecular Markers
… locus in repulsion phase was obtained by bulked segregant analysis and was converted to the SCAR marker. This marker is capable of differentiating mefenoxam resistant populations from sensitive populations included in this study.
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Molecular marker analysis of a segregating monoploid potato family
… from unreduced gametes. Simple sequence repeat analysis with eight polymorphic primer pairs was used successfully to identify the homozygous diploid plants that were added to the monoploids. In total 34 monoploid plants and 14 homozugous diploids were obtained. The degree of heterozygosity …
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A molecular genetic analysis of resistance to poleroviruses in sugar beet and oilseed rape
… the gene responsible for susceptibility by bulked segregant analysis and AFLP™. This identified a region of ca. 5Mbp region on A. thaliana chromosome 4. This region contains the Arabidopsis elongation initiation factor 4E (eIF4E) gene which has already been implicated in susceptibility to …
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The Evolution and Development of Nectar Spurs
… and the results of a recent transcriptomic analysis comparing *A. majus* to *L. vulgaris*. Candidate gene function was explored through RT-qPCR-based analysis of gene expression in the developing flowers of the long-spurred *L. becerrae* and its short-spurred relative *L. clementei*; through …
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Identification and mapping of a resistance gene to barley leaf rust(<I>Puccinia hordei</I> G. Otth)
… possesses the resistance gene R<I>ph</I>5. Bulked segregant analysis was used to identify polymorphic RFLP and SSR markers that were used for mapping in each population. Linkage analysis revealed that the R<I>ph</I>5 gene maps to barley chromosome 3 (3H) above the centromeric region in the …