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Showing 1 to 15 of 15 for “"Buffy coat"”.
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Bone Marrow-Derived Buffy Coat Can Supplement the Bone Marrow Stimulation Technique for Articular Cartilage Repair
" Bone Marrow-Derived Buffy Coat Can Supplement the Bone Marrow Stimulation Technique for Articular Cartilage Repair Bone marrow stimulation (BMS) has been used as a popular operating technique to repair defect of knee articular cartilage. However, the regenerated tissue from BMS is known as a …
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Bakterielle Kontamination von HbF-Erythrozytenkonzentraten aus Plazentarestblut
… in die Komponenten Erythrozytenkonzentrat, Buffy coat und Plasma aufgetrennt. Diese drei Fraktionen wurden an drei Tagen (1, 3, 35) auf aerobe und anaerobe bakterielle Kontamination untersucht, wobei an Tag 1 zusätzlich auch noch das Vollblut überprüft wurde. Dafür wurden Kulturflaschen mit …
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Management of cartilage deficiency by applying extracellular matrix membrane on bone marrow stimulation technique and evaluating the quality of repaired cartilage
… feasibility of autologous bone marrow-derived buffy coat transplantation in repair of large full-thickness cartilage defects was investigated in this study. Rabbits were divided into four groups: the defect remained untreated as a negative control; performance of BMS only (BMS group); BMS …
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A study of FT-IR spectroscopy for the identification and classifcation of haematological malignancies
… classified 80.6% of 331 spectra and the buffy coat model correctly classified 99.5% of 1438 spectra. This demonstrated that the buffy coat (containing white blood cells) holds the key biochemical information for discrimination between the pathology of the blood samples. Partial least …
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Dried spot cards to analyse biologic fluids for diagnostic investigation of patients
… subsequent analysis. Methods: Samples (blood, buffy coat, serum, plasma and urine) were collected into various tubes and spotted onto filter paper cards. Concentrations of total cholesterol, triglyceride, phospholipids, glucose, lactate, and protein were measured in the original sample and …
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Associations of Single Nucleotide Polymorphisms in the Bovine Prolactin, Melatonin Receptor 1A, and Dopamine Receptor D2 Genes with Hair Coat Shedding Scores and Productivity Traits in Beef Cattle
… D2 (DRD2) gene has been associated with hair coat score (HCS) in cattle. Our objective was to determine associations between mutations in the PRL, MTNR1A, and DRD2 genes and cow-calf profitability traits. Genomic DNA was extracted from buffy coat samples of Angus-based crossbred cows (n = …
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Antibody-functionalized nanoporous surfaces enable high throughput specific cell capture
… cancer lines as low as 5 cells/mL spiked into buffy coat.
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Prevention of bacterial growth in platelet products via inclusion of iron chelators
… significantly inhibited S. epidermidis growth in buffy coat platelets in a dose dependent manner (p < 0.001). With these findings, the inclusion of iron chelators is a promising approach to preventing transfusion-transmitted bacterial infection and providing patients with a safer platelet product.
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Imaging atherosclerotic plaque inflammation with [18F]- fluorodeoxyglucose positron emission tomography
… using FDG-PET. Initially, THP-1 monocytes and buffy-coat macrophages were stimulated with cellular activators, and the effect on deoxyglucose uptake was observed. It was demonstrated that both types of cell accumulated deoxyglucose in proportion to their metabolic activity. Next, FDG uptake was …
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Characterisation of the Lipidome of Transfusible Platelet Components
… and sampled on day 1, 5, and 14 post-collection. Buffy coat derived platelet components were frozen (-80°C) with 5-6% DMSO, thawed and then stored at room temperature for 24 hours, and samples were taken before freezing, after thawing and after post-thaw storage. The platelet, microparticle and …
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Interaktion des Frühsommer-Meningoenzephalitis- Virus mit antigenpräsentierenden Zellen
… DC-Subtypen und T Zellen wurden dazu aus humanen Buffy-Coat isoliert, kultiviert und mit dem FSME-Virus infiziert. Anschließend wurde die Expression von Aktivierungsmarkern sowie die Produktion von proinflammatorischen Zytokinen und antiviral wirksamen IFN-α untersucht. Dabei konnte gezeigt …
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Real Time RT-PCR for Direct Detection of Viable Mycobacterium Avium Subspecies paratuberculosis in Chron's Disease Patients and Association of Map Infection with Downregulation in Interferon-Gamma Receptor (INFG1) Gene in Crohn's Disease Patients
… from 13 CD, 1 UC and 3 NIBD subjects), 24 buffy coat blood (originated from 7 CD, 2 UC, 11 NIBD and 4 healthy subjects) and 4 intestinal biopsies from 2 CD patients. Real time RT-PCR detected viable MAP in 11/17 (65%) of iii suspected cultures compared to 12/17 (70%) by nested PCR including …
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A comprehensive study of the impact of contrast medium on endothelial cells in vitro: validating the protective effects of n-acetylcysteine and assessing the importance of endothelin-1 and glutathione on contrast-induced nephropathy in patients with chronic kidney disease
… (CKD) undergoing angiography.<br/>Plasma and buffy coat cell samples were harvested from a previously conducted clinical study. This was a comprehensive 4-part trial aiming to assess the therapeutic potential of N-acetylcysteine (NAC) as an antioxidant therapy/prophylactic to lower the impact …
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Contagious ecthyma virus infection of sheep: virologic and immunologic investigations
… skin infection. Replication of CE virus in buffy coat cells in vitro suggested that the virus may replicate in macrophages and therefore parenteral administration of vaccines may be feasible. Occurrence of CE in vaccinated sheep raised questions about possible variation of antigenic types of …
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Evaluations of Diagnostic Tests for Undifferentiated Febrile Illness on the Thailand-Myanmar (Burma) Border
… acute plasma specimens for the IgM ICT and acute buffy coat specimens for the qPCRs. Sensitivities of the IgM ICT, 47kDa qPCR and 17kDa qPCR were 27.3% (95% CI 15.0-42.8), 22.7% (95% CI 11.5-37.8) and 31.5% (95% CI 19.5-45.6) respectively. Specificities of the IgM ICT, 47kDa qPCR and 17kDa qPCR …