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Showing 1 to 5 of 5 for “"Buffer Exchange"”.
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A passive microfluidic device for continuous buffer exchange
… the cells into a low-conductivity fluid buffer. The use of a low conductivity buffer also aids in minimizing the effects of Joule heating, which can cause cell death and ineffective cell trapping. The common method to prepare the sample relies on centrifugation of sensitive cells, a …
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Optimization of Downstream Purification of Lucentis
… series of chromatography columns including anion exchange, cation exchange, and hydrophobic interaction chromatography (TOSOH Bioscience) resins followed by a 30 kDa tangential flow filtration (TFF) cassette (Pall Corp). Alternative case 1 consisted of a multimodal resin (hydrophobic and cation …
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Enhancing Micro Free Flow Electrophoresis: Detection, Application, And Fabrication
… exploring new device designs for rapid on-line buffer exchange, and developing a novel fabrication technique for hot embossing µFFE devices in cyclic olefin copolymer (COC) with the goal of expanding µFFE’s use in analytical workflows. A new laser alignment for LIF was explored, focusing the …
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Designing Magnetically Responsive Ultrafiltration Membranes
… main purposes: concentration, separation, and buffer exchange. UF membrane processes in product streams undergo frequent use and like all membrane processes experience a gradual decline in performance due to fouling phenomena both irreversible and reversible. Ultimately, performance declines to …
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Consistent Fabrication of Ultrasmall PLGA Nanoparticles and their Potential Biomedical Applications
… assembly into virus-like particles (VLPs) via buffer exchange. The size of the VLPs was monitored with and without PLGA nanoparticles present in solution during the refolding process and TEM images were collected to confirm encapsulation.