Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 181 for “"Budding Yeast"”.
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Visualization of the Budding Yeast Cell Cycle
The cell cycle of budding yeast is controlled by a complex chemically reacting network of a large group of species, including mRNAs and proteins. Many mathematical models have been proposed to unravel its molecular mechanism. However, it is hard for people with less training to visually interpret …
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Regulation of mitotic spindle biogenesis in budding yeast
… duplication (spindle pole body or SPB in yeast), separation of sister centrosomes and microtubule dynamics. Activation of Cdc28 (Cdk1) by tyrosine-19 dephosphorylation is essential for centrosome separation, the first step in bipolar spindle formation. In this study, we have investigated …
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Cell cycle regulation during gametogenesis in budding yeast
… kinase Cdc5, which activates mitotic exit in budding yeast, has gained additional roles during meiosis 1. I show that CDC5 is required for the removal of cohesin from chromosome arms in meiosis I, which is a prerequisite for meiosis I segregation. Despite the central role of CDC5 in regulating …
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Mathematical Modeling of the Budding Yeast Cell Cycle
The cell cycle of the budding yeast, Saccharomyces cerevisiae, is regulated by a complex network of chemical reactions controlling the activity of the cyclin-dependent kinases (CDKs), a family of protein kinases that drive the major events of the cell cycle. A previous mathematical model by Chen et …
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Mitotic Exit Control in Budding Yeast: Regulators and Dynamics
<p>In budding yeast, the phosphatase Cdc14 is released from nucleolus to promote mitotic exit (ME). Cdc14 release and ME is controlled by mitotic cyclin-Cdk oscillation, the FEAR network including a non-proteolytic function of separase (Esp1), and the Mitotic Exit Network (MEN) indirectly activated …
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Engineering a synthetic p53-Mdm2 network in budding yeast
… a synthetic p53 network has been built in budding yeast with the aim of studying the network in isolation while being embedded in living cells. p53 and most proteins in the network are absent from the budding yeast genome. This diminishes the likelihood of interferences on the engineered …
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Cell Polarity Establishment in the Budding Yeast Saccharomyces Cerevisiae
… GTPase Cdc42. In response to cell cycle cues the budding yeast <italic>Saccharomyces cerevisiae</italic> polarizes Cdc42p to a discrete site on the cell periphery. GTP-Cdc42p recruits a number of effectors that aid in the organization of a polarized actin cytoskeleton. The polarized actin …
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Molecular analysis of kinetochore-microtubule attachment in budding yeast
… that assemble on centromeric DNA sequences. In budding yeast, the kinetochores consist of approximately fifty proteins that are organized into a large multi-subunit complex. Multiple kinetochore proteins are thought to work together in establishing, sensing and maintaining the microtubule …
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Discovery and biochemical characterization of RNA interference in budding yeast
… to this widespread conservation of RNAi is the budding yeast Saccharomyces cerevisiae, which lacks homologs of Dicer and Argonaute. Indeed, RNAi had been presumed lost in all budding yeasts. Motivated by the presence of Argonaute homologs in some budding-yeast species, we examined whether these …
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Dissecting the spatial structure of overlapping transcription in budding yeast
… of high-resolution transcription data in the budding yeast Saccharomyces cerevisiae. We begin by describing a computational system for storing and retrieving spatially-mapped genomic data. This system forms the infrastructure for a novel algorithmic approach to detect and recover instances of …
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Mitochondrial Inheritance and Function in the Lifespan Control of Budding Yeast
… mitochondria to the bud tip and its effect on yeast lifespan determination are presented. Mitochondria at the bud tip are associated with cortical ER (cER) sheets underlying the plasma membrane. Mmr1p, a member of the DSL1 family of tethering proteins, mediates anchorage of mitochondria at the …
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Actin Cable Function and Regulation in the Budding Yeast, Saccharomyces cerevisiae
… actin cable dynamics and assembly in the budding yeast, S. cerevisiae. First, I examined the role of type II myosin and a tropomyosin isoform in retrograde actin flow (Chapter II). In yeast and other cell types, actin undergoes retrograde or centripetal movement from the cell cortex …
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Assembly and autoinhibitory mechanisms of the budding yeast kinetochore KMN complex
… KMN complex assembly and autoinhibition in the budding yeast S. cerevisiae. Using cryo-EM and biochemistry, I found that two uncharacterised -helices in C-terminal regions of the Mtw1 and Nnf1 subunits within Mtw1c contact distinct binding sites in Knl1c to mediate the interaction between Mtw1c …
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Mechanisms for maintaining genomic integrity during chromosome segregation in budding yeast
… regulate the maintenance and segregation of the budding yeast ribosomal DNA array, a highly repetitive and transcriptionally active locus. Here I show that Lrs4 and Csml bind the RENT complex at the non-transcribed space region 1 within the rDNA array and via cohesin or condensin inhibit unequal …
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Using biophysical analysis to study the regulation of the budding yeast kinetochore
Kinetochores are large macromolecular machines that serve to physically segregate the genomic material of a cell during mitosis. To achieve this, the kinetochore must make strong attachments to dynamic microtubules under force for long periods of time, but also be able to correct erroneous …
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Understanding The Mechanism of Genomic Instability During Replicative Aging In Budding Yeast
… model organism Saccharomyces cerevisiae, or budding yeast using the innovative “mother enrichment program” as the method to isolate unparalleled numbers of aged yeast cells to investigate the molecular changes associated with aging. My goal is to determine the possible causes of loss of …
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Molecular and functional characterization of tubulin isotypes in budding yeast (Saccharomyces cerevisiae)
… with a range of genome-editing techniques. Yeast is an advantageous model for tubulin isotype study because, unlike higher eukaryotes, it uses just one tubulin (TUB2) and twotubulin isotypes (TUB1 and TUB3), without PTMs, to build MTs for distinct tasks. In this dissertation, I …
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Regulation of Cell Polarity in the Budding Yeast <i>Saccharomyces cerevisiae</i>
Die Polarisierung der Saccharomyces cerevisiae-Zellen erfolgt zu verschiedenen Zeitpunkten während der Zellteilung. Zu Beginn der Zellteilung werden spezifische Orte für die Sprossung ausgewählt. Die Wahl der Sprossungsorte folgt definierten räumlichen Mustern und unterliegt der Kontrolle von …
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