Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Buccal Swab"”.
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Investigation into implementing a massively parallel sequencing workflow for forensic human identification in South Africa
… rates of analysing reference samples (crude buccal swab lysates) with the ForenSeqTM DNA Signature Prep kit. This entailed systematic adjustments to a direct PCR approach and the development of a lysate purification method. This optimised approach was subsequently used to conduct a population …
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Investigation into implementing a massively parallel sequencing workflow for forensic human identification in South Africa
… rates of analysing reference samples (crude buccal swab lysates) with the ForenSeqTM DNA Signature Prep kit. This entailed systematic adjustments to a direct PCR approach and the development of a lysate purification method. This optimised approach was subsequently used to conduct a population …
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Necrobiome Succession in Muscle Tissue as a Potential Indicator of Postmortem Interval
… to postmortem interval through the use of buccal swab and muscle tissue sampling from the arm (deltoid) and thigh (vastus lateralis). The use of muscle tissue samples is a novel means to study bacterial succession during human decomposition. Utilization of 16s rRNA sequencing provided …
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Ageing in dogs (canis familiaris) and its relationship to their environment
… correlated with the ones measured from oral swabs from the same individuals. As a correlation between samples from these two tissues were found we validated the use of buccal swab sampling to obtain dogs’ relative telomere length. We then investigated whether dogs’ relative telomere length …
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How MAOA, decision-making, and negative parenting impact aggression and conduct problems in children: a gene by environment interaction study
… of at-risk children. Measures include the buccal swab, the Iowa Gambling Task, the Teacher Report of Proactive and Reactive Aggression, the Behavior Assessment System for Children, and the Alabama Parenting Questionnaire. Analyses: Base models will test if MAOA variants, decision-making, …
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Exploring new methodologies to identify disease-associated variants in African populations through the integration of patient genotype data and clinical phenotypes derived from routine health data: A case study for Type 2 Diabetes Mellitus in patients in the Western Cape Province, South Africa
… informed consent process, DNA collection by buccal swab and DNA extraction from buccal swabs and peripheral blood samples. DNA samples were genotyped for approximately 2.2 million variants on the Infinium™ H3Africa Consortium Array V2. Genotyping quality control (QC) was done in Plink 1.9 and …
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Blood on FTA™ Paper: Does Punch Location Affect the Quality of a Forensic DNA Profile?
… to be retested. Current methods are to collect a buccal swab or blood sample, and store the DNA extracts under strict preservation conditions, i.e. cold storage, typically -20° C. With continually increasing number of samples submitted, a burden is placed on crime labs to store these DNA extracts. …
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Considering behaviour in genetic sampling – a case study with grey seals
… to cause minimal stress to the animals. Buccal swabs were tested in a group of grey seals in a rehabilitation centre. The swabs showed potential for being able to carry out individual genotype analysis to identify polymorphisms; a SNP in the grey seal DRD4 gene not previously reported in …