Global ETD Search
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Showing 1 to 15 of 15 for “"BubR1"”.
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Regulation of BubR1 by Ultraviolet Radiation: Implications in Skin Cancer
… that promote the development of skin cancer. BubR1 is an essential mitotic checkpoint protein that plays a critical role in regulating chromosome segregation and maintaining genomic stability. BubR1 dysregulation can lead to an increase in chromosomal instability and aneuploidy, a common …
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BUBR1 in Cardiac Biology: From Cardiogenesis to Adult Heart Function and Aging
… This dissertation investigates the role of BubR1, a mitotic checkpoint protein, in cardiac morphogenesis, cardiac aging, intercellular communication, and wound healing, uncovering novel regulatory pathways that contribute to both developmental and degenerative cardiac conditions. Our …
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Investigating the Relationship Between Calorie Restriction and Mitotic Protein BubR1 in the Prevention of CIN-Induced Tumorigenesis
… to aneuploidy that increases the risk of cancer. BubR1, an important mitotic protein, functions to prevent the onset of age-related phenotypes and diseases through its mitotic functions in the spindle assembly checkpoint response and stabilization of chromosome-microtubule interactions. BubR1 …
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Molecular Mechanisms of Mitotic Spindle Assembly and Accurate Chromosome Segregation
… the role of the auto-phosphorylation of BubR1, a mitotic checkpoint protein, in kinetochore-microtubule attachment and the mitotic checkpoint. Using a phospho-specific antibody against the auto-phosphorylation site identified by mass spectrometry, I demonstrate that kinetochore-associated …
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Generating the Spindle Assembly Checkpoint Signal at the Kinetochore
… complex. The roles of two kinases- BubR1 and Mps1, implicated in spindle assembly checkpoint were investigated. A checkpoint complex containing BubR1 and Bub3 has been purified from mitotic human cells. BubR1 directly interacts with Cdc20 and inhibits the activity of APC in …
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Generating the Spindle Assembly Checkpoint Signal at the Kinetochore
… complex. The roles of two kinases- BubR1 and Mps1, implicated in spindle assembly checkpoint were investigated. A checkpoint complex containing BubR1 and Bub3 has been purified from mitotic human cells. BubR1 directly interacts with Cdc20 and inhibits the activity of APC in …
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PP2A-B56 isoform specificity at the centromere and kinetochore
… PP2A-B56δ localize to the outer kinetochore (via BUBR1), whereas PP2A-B56α and PP2A-B56ε localize to the centromere (via Sgo2). The differential localization observed is due to a difference in affinity for the receptors: PP2A-B56γ has a reduced affinity for Sgo2 compared to PP2A-B56α and, vice …
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Investigating mitotic vulnerabilities that arise upon oncogenic cell transformation
… lethality screens, the mitotic proteins BuGZ and BubR1 were identified as essential for Glioblastoma Multiforme cancer cell survival but dispensable for healthy neural cell survival. We characterize the important chaperone-like role BuGZ plays in mitosis to examine its apparent dispensability in …
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The Multifunctional Kinase Bub1 Acts as a Signaling Hub for the Spindle Checkpoint
… other important checkpoint proteins like BubR1, Mad1 and Cdc20. We found phosphorylation on Bub1 serine 459 is essential for spindle checkpoint and for Bub1-Mad1 interaction. However, the majority of Mad1 still localize to the kinetochore in cells expressing Bub1-S459A mutant. These …
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Role of phosphatases in the end-on conversion process
… of the two outer-kinetochore phosphatases - BubR1- recruited PP2A-B56 and KNL1-recruited PP1 - in counteracting Aurora B kinase during the end-on conversion process. I found that BubR1-recruited PP2A-B56 plays an essential role in the process, but KNL1-recruited PP1 does not. I also …
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Safeguard of Mitosis: The Spindle Checkpoint
… checkpoint complex (MCC), which consists of BubR1-Bub3, Mad2, and Cdc20. The diffusible MCC inhibits the ubiquitin ligase activity of the anaphase-promoting complex or cyclosome bound to its co-activator Cdc20 (APC/C-Cdc20) to arrest cells in mitosis. Nevertheless, it remains unknown how the …
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The peptidyl-prolyl isomerase Pin1 is required for maintenance of the spindle assembly checkpoint
… of Cdc20 by the SAC components Mad2 and BubR1. The consequent inactivation of the APC/C causes a metaphase arrest and gives the cell time to correct the error. Given its great importance for chromosome segregation fidelity, it comes at no surprise that loss of the SAC causes cell death …
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Molecular Mechanisms of Mitotic Checkpoint Complex Assembly onto Kinetochores
… cyclin B and securin. The MCC is composed of BubR1, Cdc20 and Mad2, and while their assembly is an intrinsically very slow process, in cells it is catalytically activated. Recent work points towards hierarchical recruitments of SAC proteins onto the outer kinetochore by means of a …
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Mitotic slippage by ATP depletion and its mechanism
… spindle assembly checkpoint proteins including BubR1, Mad2, and MCAK. However, the localization and the kinase activity of Aurora B, which is widely recognized as a component of error correction of microtubule attachment and its role in maintenance of spindle assembly checkpoint, were reduced on …