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Showing 1 to 6 of 6 for “"Bub1"”.
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The Multifunctional Kinase Bub1 Acts as a Signaling Hub for the Spindle Checkpoint
… by preventing Cdc20 from activating APC/C. Bub1 is a highly conserved spindle checkpoint protein that plays multiple roles in checkpoint signaling. On the kinetochore, Bub1 recruits other important checkpoint proteins like BubR1, Mad1 and Cdc20. We found phosphorylation on Bub1 serine 459 is …
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Safeguard of Mitosis: The Spindle Checkpoint
… complexes budding uninhibited by benomyl 1-3 (Bub1-Bub3) and Bub1-related protein in complex with Bub3 (BubR1-Bub3) to kinetochores. My following work has demonstrated that Mps1 promotes the inhibition of APC/CCdc20 by MCC components in vitro through phosphorylating Bub1 and mitosis arrest …
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Molecular Mechanisms of Mitotic Checkpoint Complex Assembly onto Kinetochores
… to explore the structure and function of the Bub1- Mad1 complex, including how sequential phosphorylation of the Bub1 CD1 domain by Cdk1 and Mps1 promotes kinetochore targeting of the Mad1:C-Mad2 complex. Chapter 4 investigates how Mad1 C-terminal phosphorylation by Mps1 promotes juxtaposition …
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Identification and characterization of novel genes involved in DNA double strand break repair process in the yeast Saccharomyces cerevisiae
… process. Among these genes, PPH3/PSY2, HUR1, BUB1/BUB2, and SBP1 were selected for follow up experiments. Small-scale plasmid repair and chromosomal break repair assays were used to verify the involvement of these selected candidates in the efficiency of NHEJ. Deletion of any one of the above …
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Generating the Spindle Assembly Checkpoint Signal at the Kinetochore
… mitosis whereas the kinetochore localization of Bub1 and BubR1 is largely unaffected. Interestingly, the kinetochore localization of Mad1 in cells with a compromised Ndc80 function is restored upon microtubule depolymerization. Thus, hSpc25 is an essential kinetochore component that plays a …
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Generating the Spindle Assembly Checkpoint Signal at the Kinetochore
… mitosis whereas the kinetochore localization of Bub1 and BubR1 is largely unaffected. Interestingly, the kinetochore localization of Mad1 in cells with a compromised Ndc80 function is restored upon microtubule depolymerization. Thus, hSpc25 is an essential kinetochore component that plays a …